Evaluation of differentially expressed microRNAs in vitrified oocytes by next generation sequencing

Li, Jun; Yang, Xuemei; Liu, Fang; Song, Yaman; Liu, Yuanke

doi:10.1016/j.biocel.2019.05.006

Cited by 13 publications

(8 citation statements)

References 33 publications

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“…We select the Rfam database to annotate the small RNA sequences obtained by sequencing, and find and remove the possible rRNA, snoRNA, snRNA, tRNA, and other non-miRNA sequences as far as possible; (4) miRNA identification: after effective data were obtained, the precursor and genome were compared for miRNA identification; the t -test test was used in this analysis. For the analysis of samples with biological duplicates, the threshold of P ≤ 0.05 was used to screen differentially expressed genes ( Han et al, 2019 ; Jeon et al, 2019 ; Li et al, 2019 ); (5) identification of differentially expressed miRNAs; and (6) prediction of differential miRNA target genes. The data presented in the study are deposited in the GEO respository ( https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE182429 ).…”

Section: Methodsmentioning

confidence: 99%

Dysregulation of Principal Circulating miRNAs in Non-human Primates Following Ischemic Stroke

et al. 2021

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Despite the recent interest in plasma microRNA (miRNA) biomarkers in acute ischemic stroke patients, there is limited knowledge about the miRNAs directly related to stroke itself due to the multiple complications in patients, which has hindered the research progress of biomarkers and therapeutic targets of ischemic stroke. Therefore, in this study, we compared the differentially expressed miRNA profiles in the plasma of three rhesus monkeys pre- and post-cerebral ischemia. After cerebral ischemia, Rfam sequence category revealed increased ribosomic RNA (rRNA) and decreased transfer RNAs (tRNAs) in plasma. Of the 2049 miRNAs detected after cerebral ischemia, 36 were upregulated, and 76 were downregulated (fold change ≥2.0, P < 0.05). For example, mml-miR-191-5p, miR-421, miR-409-5p, and let-7g-5p were found to be significantly overexpressed, whereas mml-miR-128a-5p_R − 2, miR-431_R − 1, and let-7g-3p_1ss22CT were significantly downregulated. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses revealed that these differentially expressed miRNAs were implicated in the regulation of ubiquitin-mediated proteolysis and signaling pathways in cancer, glioma, chronic myeloid leukemia, and chemokine signaling. miRNA clustering analysis showed that mml-let-7g-5p and let-7g-3p_1ss22CT, which share three target genes [RB1-inducible coiled-coil 1 (RB1CC1), G-protein subunit γ 5 (GNG5), and chemokine (C-X-C motif) receptor 4 (CXCR4)], belong to one cluster, were altered in opposite directions following ischemia. These data suggest that circulating mml-let-7g may serve as a therapeutic target for ischemic stroke.

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Section: Methodsmentioning

confidence: 99%

Dysregulation of Principal Circulating miRNAs in Non-human Primates Following Ischemic Stroke

et al. 2021

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“…Furthermore, no significant differences were seen in cleavage timing or predictive morphokinetic time intervals between embryos developed from fresh and vitrified oocytes evaluated under time-lapse monitoring [156]. Another point of epigenetic issue, noncoding RNA (miRNA) expression in fresh/vitrified human oocytes was reported in 2019 [157]. At least 22 miRNAs differed between fresh and vitrified oocytes, e.g., miR-134-5p, miR-210-5p, miR-21-3p and miR-465c-5p, which target the PTEN gene (cell apoptosis regulation through oxidative stress pathway) [157].…”

Section: Humansmentioning

confidence: 99%

“…Another point of epigenetic issue, noncoding RNA (miRNA) expression in fresh/vitrified human oocytes was reported in 2019 [157]. At least 22 miRNAs differed between fresh and vitrified oocytes, e.g., miR-134-5p, miR-210-5p, miR-21-3p and miR-465c-5p, which target the PTEN gene (cell apoptosis regulation through oxidative stress pathway) [157]. However, a small sample size was used in these novel genome reports, and correlation between gene expression and other oocyte quality parameters were rarely determined [155].…”

Section: Humansmentioning

confidence: 99%

Oocyte Cryopreservation in Domestic Animals and Humans: Principles, Techniques and Updated Outcomes

Tharasanit

Thuwanut

2021

Animals

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Oocyte cryopreservation plays important roles in basic research and the application of models for genetic preservation and in clinical situations. This technology provides long-term storage of gametes for genetic banking and subsequent use with other assisted reproductive technologies. Until recently, oocytes have remained the most difficult cell type to freeze, as the oocytes per se are large with limited surface area to cytoplasm ratio. They are also highly sensitive to damage during cryopreservation, and therefore the success rate of oocyte cryopreservation is generally poor when compared to noncryopreserved oocytes. Although advancement in oocyte cryopreservation has progressed rapidly for decades, the improvement of cryosurvival and clinical outcomes is still required. This review focuses on the principles, techniques, outcomes and prospects of oocyte cryopreservation in domestic animals and humans.

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“…A recent study conducted in mice reported the comparison of miRNA transcriptome in fresh and vitrified oocytes [120]. Twenty-two miRNAs were differentially expressed between the two groups, and most of the target genes regulated by these miRNAs were identified as "metabolic pathway" regulators.…”

Section: Micrornasmentioning

confidence: 99%

“…Among them, miR-134-5p, miR-210-5p, and miR-21-3p were significantly upregulated, whereas miR-465c-5p was downregulated. The expression of potential target PTEN, regulating cell apoptosis through oxidative stress, was reduced [120].…”

Section: Micrornasmentioning

confidence: 99%

What impact does oocyte vitrification have on epigenetics and gene expression?

Barberet¹,

Barry²,

Choux³

et al. 2020

Clin Epigenet

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Children conceived by assisted reproductive technologies (ART) have a moderate risk for a number of adverse events and conditions. The question whether this additional risk is associated with specific procedures used in ART or whether it is related to the intrinsic biological factors associated with infertility remains unresolved. One of the main hypotheses is that laboratory procedures could have an effect on the epigenome of gametes and embryos. This suspicion is linked to the fact that ART procedures occur precisely during the period when there are major changes in the organization of the epigenome. Oocyte freezing protocols are generally considered safe; however, some evidence suggests that vitrification may be associated with modifications of the epigenetic marks. In this manuscript, after describing the main changes that occur during epigenetic reprogramming, we will provide current information regarding the impact of oocyte vitrification on epigenetic regulation and the consequences on gene expression, both in animals and humans. Overall, the literature suggests that epigenetic and transcriptomic profiles are sensitive to the stress induced by oocyte vitrification, and it also underlines the need to improve our knowledge in this field.

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Evaluation of differentially expressed microRNAs in vitrified oocytes by next generation sequencing

Cited by 13 publications

References 33 publications

Dysregulation of Principal Circulating miRNAs in Non-human Primates Following Ischemic Stroke

Dysregulation of Principal Circulating miRNAs in Non-human Primates Following Ischemic Stroke

Oocyte Cryopreservation in Domestic Animals and Humans: Principles, Techniques and Updated Outcomes

What impact does oocyte vitrification have on epigenetics and gene expression?

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