Dysregulation of Principal Circulating miRNAs in Non-human Primates Following Ischemic Stroke

Chen, Jian; Zhao, Haiping; Huang, Yuyou; Li, Yuqian; Fan, Junfen; Wang, Rongliang; Han, Ziping; Yang, Zhenhong; Wu, Longfei; Wu, Di; Luo, Yumin; Ji, Xunming

doi:10.3389/fnins.2021.738576

Cited by 3 publications

(2 citation statements)

References 35 publications

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“…Let-7g-3p overexpression induces cell cycle block and delays the progression of EMT [51,52], while its decreases promote the production of pro-inflammatory cytokines [53]. Let-7d has been reported to be involved in the initiation and progression of IPF, negatively regulating the fibroblast-forming effect to inhibit pulmonary fibrosis [54].…”

Section: Discussionmentioning

confidence: 99%

Bioinformatics Analysis of the Cross-talk Between Idiopathic Pulmonary Fibrosis and Hashimoto's Thyroiditis on the Hub Genes and Key Pathways

Ding

Kong

et al. 2022

Preprint

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Background: Idiopathic pulmonary fibrosis (IPF) is a disease of unknown cause, and there is increasing evidence that Hashimoto's thyroiditis (HT) is closely related to IPF. However, the mechanism of their interaction relationship remains unclear. The purpose of this study was to explore the common genetic features and underlying molecular and immune mechanisms between HT and IPF. Methods: Microarray data of HT and IPF were obtained from the Gene Expression Omnibus (GEO) database. The R software was used to identify the differential genes between two datasets and take the common differential genes (DEGs). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses of common DEGs present in HT and IPF using R software. The protein-protein interaction (PPI) network of their DEGs was constructed Utilizing the Search Tool for Retrieval of Interacting Genes/Proteins (STRING) database, identified and visualized hub genes by the Cytoscape software. To further investigate the diagnostic value of the hub genes, the receptor operating characteristic (ROC) curves were constructed utilizing the ‘pROC’ package. Subsequently, ROC analysis in two independent GEO datasets further validates the diagnostic value of these six hub genes. A miRNA-gene interactions network, TF-gene interactions network, and TF-miRNA coregulatory network were constructed by the NetworkAnalyst using hub genes and visualized by the Cytoscape for further analysis. In addition, R software was also applied to analyze immunological infiltration. Results: A total of 572 common DEGs were identified from the two datasets for subsequent analysis, which were primarily enriched in immune and inflammatory-related pathways. The plug-in ‘cytoHubba’ of the Cytoscape software was utilized to recognize six shared hub genes after validation, including CYBB, CD68, CD8A, CCL2, CCL5, and PTPRC. We predicted the miRNAs related to hub genes, including miR-106b-5p, miR-155-5p, miR-98-5p, let-7g-3p, and potential TFs (SPI1, NFKB1, NFKB2) through the interaction network. In addition, immune cell infiltration analysis revealed a significant infiltration of central memory CD4+ T cells infiltration in both IPF and HT samples. Conclusions: Bioinformatics studies reveal the common pathogenesis and immunological features between Hashimoto's thyroiditis and idiopathic pulmonary fibrosis. These common pathways and hub genes may serve as molecular biomarkers for diagnosable as well as prognostic assessment, providing new targets and ideas for further mechanistic studies. Additionally, the immune system plays a crucial role in the initiation and progression of both diseases. Keywords: idiopathic pulmonary fibrosis, Hashimoto's Thyroiditis, hub gene, immune process, bioinformatics

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Section: Discussionmentioning

confidence: 99%

Bioinformatics Analysis of the Cross-talk Between Idiopathic Pulmonary Fibrosis and Hashimoto's Thyroiditis on the Hub Genes and Key Pathways

Ding

Kong

et al. 2022

Preprint

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“…Lower miR-191-5p values were prospectively associated with an enhanced risk of reinfarction ( 18 ). Conversely, elevated circulating miR-191-5p levels were found in an animal model of ischemic stroke ( 19 ) and in children with nephrotic syndrome ( 20 ).…”

mentioning

confidence: 99%

Serum MicroRNA-191-5p Levels in Vascular Complications of Type 1 Diabetes: The EURODIAB Prospective Complications Study

Bellini

Guarrera

Matullo

et al. 2023

The Journal of Clinical Endocrinology &Amp; Metabolism

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Context MicroRNA-191-5p regulates key cellular processes involved in the pathogenesis of diabetic complications such as angiogenesis, extracellular matrix deposition, and inflammation. However, no data on circulating microRNA-191-5p in the chronic complications of diabetes are currently available. Objective To assess whether serum levels of miR-191-5p were associated with micro- and macrovascular disease in a large cohort of subjects with type 1 diabetes (DM1) from the EURODIAB Prospective Complication Study (PCS). Design and Setting Levels of microRNA-191-5p were measured by quantitative PCR (qPCR) in 420 DM1 subjects recruited as part of the cross-sectional analysis of the EURODIAB PCS. Cases (n = 277) were subjects with nephropathy and/or retinopathy and/or cardiovascular disease (CVD). Controls (n = 143) were patients without complications. Logistic regression analysis was performed to evaluate the potential independent association of microRNA-191-5p levels with chronic complications of diabetes. Results Levels of microRNA-191-5p were significantly reduced (P < 0.001) in cases compared to controls even after adjustment for age, sex, and diabetes duration. Logistic regression analysis revealed that microRNA-191-5p was negatively associated with a 58% reduced odd ratio (OR) of chronic diabetes complications, specifically CVD, micro-macroalbuminuria, and retinopathy [OR: 0.42 95% CI (0.23-0.77)], independently of age, sex, physical activity, educational levels, diabetes duration, glycated haemoglobin (HbA1c), total insulin dose, hypertension, smoking, total cholesterol, albumin excretion rate (AER), estimated glomerular filtration rate (eGFR), serum Vascular Cell Adhesion Molecule-1 (sVCAM-1), and tumour necrosis factor-α (TNF-α). Analyses performed separately for each complication demonstrated a significant independent association with albuminuria [OR: 0.36 95% CI (0.18-0.75)], and CVD [OR: 0.34 95% CI (0.16-0.70)]. Conclusions In DM1 subjects, miR-191-5p is inversely associated with vascular chronic complications of diabetes.

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circRNA from APP Gene Changes in Alzheimer’s Disease Human Brain

Urdánoz‐Casado

Gordoa

Robles

et al. 2023

IJMS

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Alzheimer’s disease (AD) is the most common cause of age-related dementia. Amyloid precursor protein (APP) is the precursor of Aβ peptides, and its role in AD has been widely investigated. Recently, it has been reported that a circular RNA (circRNA) originated from APP gene can serve as a template for Aβ synthesis, postulating it as an alternative pathway for the Aβ biogenesis. Moreover, circRNAs play important roles in brain development and in neurological diseases. Therefore, our aim was to study the expression of a circAPP (hsa_circ_0007556) and its linear cognate in AD human entorhinal cortex, a brain region most vulnerable to AD pathology. First, we confirmed the presence of circAPP (hsa_circ_0007556) in human entorhinal cortex samples using RT-PCR and Sanger sequencing of PCR products. Next, a 0.49-fold decrease in circAPP (hsa_circ_0007556) levels was observed in entorhinal cortex of AD cases compared to controls (p-value < 0.05) by qPCR. In contrast, APP mRNA expression did not show changes in the entorhinal cortex between AD cases and controls (Fold-change = 1.06; p-value = 0.81). A negative correlation was found between Aβ deposits and circAPP (hsa_circ_0007556) and APP expression levels (Rho Spearman = −0.56, p-value < 0.001 and Rho Spearman = −0.44, p-values < 0.001, respectively). Finally, by using bioinformatics tools, 17 miRNAs were predicted to bind circAPP (hsa_circ_0007556), and the functional analysis predicted that they were involved in some pathways, such as the Wnt-signaling pathway (p = 3.32 × 10−6). Long-term potentiation (p = 2.86 × 10−5), among others, is known to be altered in AD. To sum up, we show that circAPP (hsa_circ_0007556) is deregulated in the entorhinal cortex of AD patients. These results add to the notion that circAPP (hsa_circ_0007556) could be playing a role in the pathogenesis of AD disease.

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Dysregulation of Principal Circulating miRNAs in Non-human Primates Following Ischemic Stroke

Cited by 3 publications

References 35 publications

Bioinformatics Analysis of the Cross-talk Between Idiopathic Pulmonary Fibrosis and Hashimoto's Thyroiditis on the Hub Genes and Key Pathways

Bioinformatics Analysis of the Cross-talk Between Idiopathic Pulmonary Fibrosis and Hashimoto's Thyroiditis on the Hub Genes and Key Pathways

Serum MicroRNA-191-5p Levels in Vascular Complications of Type 1 Diabetes: The EURODIAB Prospective Complications Study

circRNA from APP Gene Changes in Alzheimer’s Disease Human Brain

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