Evaluation of different temporary immersion systems (BIT®, BIG, and RITA®) in the micropropagation of Vanilla planifolia Jacks

Ramírez-Mosqueda, Marco A.; Iglesias-Andreu, Lourdes Georgina

doi:10.1007/s11627-015-9735-4

Cited by 53 publications

(17 citation statements)

References 26 publications

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“…The large-scale cultivation of differentiated plant cultures using a liquid culture in 'Temporary Immersion Systems' (TIS), under controlled environmental conditions, recently received a rapid increase in interest worldwide (Mehrotra et al 2007;Carvalho et al 2019). Over time, different TIS bioreactors have been proposed (Georgiev et al 2014;Ramírez-Mosqueda, Iglesias-Andreu 2016), among which ones based on a single container (e.g., RITA ® , Plantform ™ ), or on double containers (e.g., twin flasks, SETIS ™ ) can be found. These bioreactors allow the plant cultures to have temporary access to the liquid medium, with other advantages regarding the nutrient absorption from the entire surface of the explant and the dilution of the phenols that can determine the oxidation and browning of the tissues (Lambardi 2012;Capuana et al 2018).…”

mentioning

confidence: 99%

Evaluation of ElecTIS bioreactor for the micropropagation of Malus sylvestris (L.) Mill., an important autochthonous species of Albania

Sota¹,

Benelli̇²,

Ҫuko³

et al. 2021

Hort. Sci. (Prague)

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Grapevine Pinot gris virus (GPGV) has been identified in many grape growing countries of the world since 2012. The aim of this work was to investigate the presence of GPGV on some accessions collected from a germplasm collection, in addition to the propagation material and clonal selection samples. During 2019–2020, a total of 199 samples have been analysed by a double antibody sandwich – enzyme-linked immunosorbent assay (DAS-ELISA) for the presence of GPGV, Grapevine fanleaf virus (GFLV), Grapevine leafroll-associated virus-1+3 (GLRaV-1+3) and Grapevine fleck virus (GFkV). Among them, 107 samples (53.76%) showed a GPGV-infection, associated with or without symptoms on the leaves (deformations, chlorosis, mosaic, wrinkles) or stunting plants. The distribution of infected varieties showed a high rate of infection in old varieties (37.38%), followed by clones (32.71%), rootstocks (11.21%), clonal selections (9.35%) and new varieties (9.35%). The tests revealed the association of GPGV with GFkV (5 cases) and GLRaV-1+3 (2 cases). GPGV should be included in the rules of grapevine certification schemes for the production of virus-free mother plants.

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mentioning

confidence: 99%

Evaluation of ElecTIS bioreactor for the micropropagation of Malus sylvestris (L.) Mill., an important autochthonous species of Albania

Sota¹,

Benelli̇²,

Ҫuko³

et al. 2021

Hort. Sci. (Prague)

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“…The authors noted that, although the PlantForm has a larger space to allow for material enlargement rather than RITA, it is necessary to carefully set the flooding conditions with the PlantForm system to obtain good quality plants suitable for the acclimatization phase in greenhouse; flooding every 8 h guaranteed a better behavior and quality without hyperhydricity. A smaller number of explant per shoots (11.8 and 8.47) was obtained by Ramírez-Mosqueda and Iglesias-Andreu [95] and Bayraktar [97], respectively, always using the RITA for the micropropagation of stevia. Furthermore, three TIS bioreactors, SETIS, RITA and BIT, were compared for the propagation of two stevia cultivars and their performance in the field was evaluated (Rosales et al, 2018).…”

Section: Types Of Tis Bioreactors and Biomass Productionmentioning

confidence: 85%

“…Pistacia lentiscus, Stevia rebaudiana, and Vanilla plantifolia showed a significantly improved multiplication rate when grown in TIS compared to conventional semi-solid culture system [88,95,101]. In particular, shoots of Vanilla plantifolia produced the maximum multiplication rate (14.27 shoots per explant) when using TIS, with an immersion frequency of 2 min every 4 h, i.e., 2.5 times higher than the multiplication rate on semi-solid medium (5.80 shoots for explant).…”

Section: Culture System and Biomass Productionmentioning

confidence: 99%

Temporary Immersion System for Production of Biomass and Bioactive Compounds from Medicinal Plants

et al. 2021

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The cultivation of medicinal plants and the production of bioactive compounds derived from them are of fundamental importance and interest, not only at the pharmacological level but also in nutraceutical and cosmetic industries and in functional foods, as well as plant protection in agriculture. In order to respond adequately to the increased demands of the global market from a quantitative and qualitative point of view and to guarantee environmental sustainability of the productions, it is necessary to resort to innovation tools, such as tissue culture in vitro technology. Nowadays, it is well known that the cultivation through the Temporary Immersion System (TIS) in a bioreactor has considerable advantages both for the in vitro mass production of the plants and for the production of secondary metabolites. The present review focuses on the application of TIS during the last two decades to produce biomass and bioactive compounds from medicinal plants. Indeed, almost one hundred papers are discussed, and they particularly focus on the effects of the culture system, vessel design and equipment, immersion time and frequency, and substrate composition for 88 medicinal species in TIS bioreactor culture.

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“…Advances in in vitro culture techniques such as TIS have recently been developed to improve mass propagation efficiency that was difficult to accomplish using conventional propagation systems such as SSS or CIS [17]. TIS mass propagation success has previously been reported for various plant species [18,19]-including orchids [11,20,21]. Prior reports revealed that different culture systems resulted in diverse growth and morphogenesis of cultured plants [11,14,22].…”

Section: Discussionmentioning

confidence: 99%

In Vitro Propagation of Rheophytic Orchid, Epipactis flava Seidenf.—A Comparison of Semi-Solid, Continuous Immersion and Temporary Immersion Systems

Kunakhonnuruk

Inthima

Kongbangkerd

2019

Biology

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Epipactis flava Seidenf. is an endangered Thai rheophytic orchid that has recently shown a rapid decrease in its natural habitat, prompting an urgent need for conservation using ex situ reintroduction methods. Temporary immersion system (TIS) has been successfully applied for large-scale propagation in various plants species. Propagation efficiency of E. flava using TIS was investigated and compared with conventional semi-solid system (SSS) and liquid continuous immersion system (CIS). The highest percentage of new shoot and shoot bud formation was obtained from TIS, followed by CIS and SSS, respectively. Growth parameters as indicated by number of new shoots, shoot buds, shoot height and leaves per explant were significantly higher using TIS than with SSS and CIS. Moreover, the maximum number of new shoots and shoot buds per replication were reliably obtained from TIS higher than SSS and CIS. After acclimatization, the highest survival percentage of plantlets was observed in TIS (76.7%), with 60% surviving after eight weeks of transplantation in artificial stream. TIS was determined as the most suitable culture system for in vitro mass propagation of E. flava compared to CIS and SSS.

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Evaluation of different temporary immersion systems (BIT®, BIG, and RITA®) in the micropropagation of Vanilla planifolia Jacks

Cited by 53 publications

References 26 publications

Evaluation of ElecTIS bioreactor for the micropropagation of Malus sylvestris (L.) Mill., an important autochthonous species of Albania

Evaluation of ElecTIS bioreactor for the micropropagation of Malus sylvestris (L.) Mill., an important autochthonous species of Albania

Temporary Immersion System for Production of Biomass and Bioactive Compounds from Medicinal Plants

In Vitro Propagation of Rheophytic Orchid, Epipactis flava Seidenf.—A Comparison of Semi-Solid, Continuous Immersion and Temporary Immersion Systems

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