Evaluation of different phenotypic methods for the detection of methicillin resistant Staphylococcus aureus and antimicrobial susceptibility pattern of MRSA

Sharma, Sheetal; Srivastava, Preeti; Kulshrestha, Anjali; Abbas, A. M. A.

doi:10.18203/2394-6040.ijcmph20173832

Cited by 11 publications

(11 citation statements)

References 3 publications

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“…92.31% of MRSA isolates were found sensitive to clindamycin which corresponds to the findings of Arjyal et al (2020) [22] who reported 100% sensitivity of MRSA isolates towards clindamycin. In line with present observation Selvabai et al (2017) [20] , Sharma et al (2017) [28] , Li et al (2017) [27] , Ahmad et al (2015) [19] , Al-Zaidi and Al-Sulami (2013) [15] and Kumar et al, (2011) [33] reported fairly similar rate of sesnistivity towards clindamycin. Whereas higher rate of resistance towards clindamycin was reported by Krishnan et al (2019) [21] , Sohail and Latif (2018) [34] and Goyal et al (2013) [24] .…”

Section: Antibiogram Of Mrsasupporting

confidence: 86%

“…Existance of vancomycin resistant MRSA during present study was well supported by Shah et al (2019) [18] , Nasution et al (2018) [17] and Al-Zaidi and Al-Sulami (2013) [15] . In contrast, Mahmood et al (2010) [23] , Selvabai et al (2017) [20] , Sonth et al (2015) [26] , Li et al (2017) [27] , Sharma et al (2017) [28] and Krishnan et al (2019) [21] reported 100% sensitivity of MRSA to vancomycin. Vancomycin, a glycopeptide antibiotic usually remains a drug of choice for treatment of MRSA infections.…”

Section: Antibiogram Of Mrsamentioning

confidence: 99%

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Antibiogram of methicillin resistant Staphylococcus aureus (MRSA) of animal origin from Chhattisgarh

Sannat¹,

Hirpurkar²,

Hazari³

et al. 2021

Pharma Innovation

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Present investigation was made to study antibiogram profile of Methicillin Resistant Staphylococcus aureus (MRSA) of animal origin. MRSA isolates were obtained from bovine mastitis and wound infection of animals using cultural isolation and were confirmed by S. aureus specific thermonuclease (nuc) and MRSA specific mecA gene in PCR assay. A total of 13 MRSA isolates were obtained and subjected to antibiotic sensitivity test using 10 numbers of selected antibiotics by disc diffusion test. MRSA isolates exhibited variable degree of sensitivity towards various antibiotics. MRSA isolates were detected 100% senisitive to linezolid and imipenem followed by clindamycin (92.31%), tetracycline (76.92%), vancomycin and gentamycin (53.85%). Isolates showed higher rate of resistance towards amoxicillin (84.62%) and penicillin (76.92%). All the isolates were found resistant to cefoxitin and methicillin. It can be concluded from present study that isolates harbouring mecA/ MRSA gene have tendency to exhibit resistant towards multidrug and treatment option can be explored by studying antibiogram of MRSA of particular region.

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Section: Antibiogram Of Mrsasupporting

confidence: 86%

Section: Antibiogram Of Mrsamentioning

confidence: 99%

Antibiogram of methicillin resistant Staphylococcus aureus (MRSA) of animal origin from Chhattisgarh

Sannat¹,

Hirpurkar²,

Hazari³

et al. 2021

Pharma Innovation

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“…Similarly, susceptibility testing using cefoxitin, a stronger inducer of the mecA regulatory system than oxacillin that is used to detect methicillin resistance 42 , failed to accurately identify OS-MRSA (Table 1). These observations suggest that a combination of oxacillin and cefoxitin susceptibility tests, as recommended elsewhere 43 , or detection of mecA will be more reliable for the identification of MRSA. Despite being phenotypically susceptible to oxacillin, β-lactam resistance can easily be induced in OS-MRSA 20,21,44 .…”

Section: Discussionmentioning

confidence: 64%

Identification and characterization of mutations responsible for the β-lactam resistance in oxacillin-susceptible mecA-positive Staphylococcus aureus

Boonsiri

Watanabe

Tan

et al. 2020

Sci Rep

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Staphylococcus aureus strains that are susceptible to the β-lactam antibiotic oxacillin despite carrying mecA (OS-MRSA) cause serious clinical problems globally because of their ability to easily acquire β-lactam resistance. Understanding the genetic mechanism(s) of acquisition of the resistance is therefore crucial for infection control management. For this purpose, a whole-genome sequencing-based analysis was performed using 43 clinical OS-MRSA strains and 100 mutants with reduced susceptibility to oxacillin (MICs 1.0–256 µg/mL) generated from 26 representative OS-MRSA strains. Genome comparison between the mutants and their respective parent strains identified a total of 141 mutations in 46 genes and 8 intergenic regions. Among them, the mutations are frequently found in genes related to RNA polymerase (rpoBC), purine biosynthesis (guaA, prs, hprT), (p)ppGpp synthesis (relSau), glycolysis (pykA, fbaA, fruB), protein quality control (clpXP, ftsH), and tRNA synthase (lysS, gltX), whereas no mutations existed in mec and bla operons. Whole-genome transcriptional profile of the resistant mutants demonstrated that expression of genes associated with purine biosynthesis, protein quality control, and tRNA synthesis were significantly inhibited similar to the massive transcription downregulation seen in S. aureus during the stringent response, while the levels of mecA expression and PBP2a production were varied. We conclude that a combination effect of mecA upregulation and stringent-like response may play an important role in acquisition of β-lactam resistance in OS-MRSA.

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“…The MIC lesser than or equal to 2 microgram was considered sensitive and and greater than or equal to 4 microgram was considered resistant. 17…”

Section: E-strip Methodsmentioning

confidence: 99%

Phenotypic and Genotypic Detection of Methicillin Resistant Staphylococcus aureus in Health Care Workers and Its Containment in a Tertiary Care Hospital, in South India

Rahman¹,

Kashid²

2021

J Pure Appl Microbiol

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MRSA causes nosocomial and community based infections. It is associated with significant mortality and morbidity. Resistance in MRSA is encoded by mecA gene. Anterior nares are the ecological niche of Staphylococcus aureus. HCWs who are colonized with MRSA, act as agents of cross contamination of hospital and community acquired MRSA. Treating MRSA infections is a therapeutic challenge as it is resistant to beta lactam group of drugs. Therefore, there is a need for rapid and accurate detection of MRSA carriage in HCWs and to understand its antibiotic susceptibility pattern.The objective of the present study is to estimate the occurrence of MRSA in HCWs, using phenotypic and genotypic methods. A prospective study for six months was conducted after obtaining Institutional Ethical Committee clearance. Anterior nasal swabs of those HCWs who gave informed consent were taken processed for culture and sensitivity as per standard protocol. To detect MIC for oxacillin, E-strip method was used. mecA gene detection was done by PCR. A total of 300 HCWs were sampled.14.66% (44/300) of the isolates were identified as Staphylococcus aureus, of which 10 isolates were detected as MRSA. The overall isolation rate of MRSA is 3.33 %(10/300). MRSA carriage was high amongst nurses (5/59, 8.47%), followed by doctors (4/105, 3.80%).Antibiotic sensitivity pattern showed that highest resistance was to penicillin (75%) followed by amoxiclav (70.45 %).9 MRSA isolates were detected as mecA gene positive by PCR. MRSA carriers were decontaminated successfully with 2% mupirocin ointment and 2% chlorhexidine shampoo. This study reiterates the need for rapid and accurate identification of HCWs who have nasal colonization with MRSA, for reinforcing hospital infection control measures and decontamination protocol. This will help prevent the spread of MRSA in our community.

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Evaluation of different phenotypic methods for the detection of methicillin resistant Staphylococcus aureus and antimicrobial susceptibility pattern of MRSA

Cited by 11 publications

References 3 publications

Antibiogram of methicillin resistant Staphylococcus aureus (MRSA) of animal origin from Chhattisgarh

Antibiogram of methicillin resistant Staphylococcus aureus (MRSA) of animal origin from Chhattisgarh

Identification and characterization of mutations responsible for the β-lactam resistance in oxacillin-susceptible mecA-positive Staphylococcus aureus

Phenotypic and Genotypic Detection of Methicillin Resistant Staphylococcus aureus in Health Care Workers and Its Containment in a Tertiary Care Hospital, in South India

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