“…High performance thin layer chromatography [10] was also evaluated, resulting in the detection of edelfosine to a limit of quantitation (LOQ) of about 26 ng of injected sample (0.3 ng with 0.1 mL of sample), inadequate to perform a pharmacokinetic analysis. High performance liquid chromatography combined with light scattering detection was investigated as an alternative technique for the quantitation of edelfosine [11]. The drawback of this method is that it can only be used for studies of relatively high-concentration edelfosine samples, such as pharmaceutical samples.…”