Evaluation of different packings for high-performance liquid chromatographic analysis of alkyl lysophospholipids

Comparative study of A HPLC–MS assay versus an UHPLC–MS/MS for anti-tumoral alkyl lysophospholipid edelfosine determination in both biological samples and in lipid nanoparticulate systems

“…High performance thin layer chromatography [10] was also evaluated, resulting in the detection of edelfosine to a limit of quantitation (LOQ) of about 26 ng of injected sample (0.3 ng with 0.1 mL of sample), inadequate to perform a pharmacokinetic analysis. High performance liquid chromatography combined with light scattering detection was investigated as an alternative technique for the quantitation of edelfosine [11]. The drawback of this method is that it can only be used for studies of relatively high-concentration edelfosine samples, such as pharmaceutical samples.…”