Capillary GC analysis of alkyl lysophospholipids after derivatization with trimethylsilylbromide

Coene, J.; Herdewijn, Piet; Eeckhout, E. Van den; Bossche, W. Van den; Sandra, Pat

doi:10.1002/jhrc.1240141015

Cited by 6 publications

(7 citation statements)

References 4 publications

(2 reference statements)

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“…Instrumentation. Samples derivatized with trimethylsilylbromide were injected into a Hewlett-Packard Series II gas chromatograph (8)(9)(10). A Beckman LS 3801 (CA, USA) scintillation counter was used for the thymidine incorporation and sialyltransferase activity measurements.…”

Section: Methodsmentioning

confidence: 99%

Edelfosine protects precultured heart fragments against the invasion of malignant cells through altered sialylation

Slambrouck

Steelant²,

Lopez³

et al. 2007

Oncol Rep

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1-O-octadecyl-2-O-methylglycero-3-phosphocholine (ET-18-OMe)-treated precultured heart fragments (PHF) are resistant to the invasion of malignant cells. Previous studies have demonstrated that this effect is due to the alterations of the N-linked glycoproteins in PHF after 48-h ET-18-OMe treatment. Moreover, the observed effect was still present seven days after ET-18-OMe was omitted. The present study reveals that ~13.4% of the administered ET-18-OMe was taken up by PHF and about 75% of the initial uptake was still present after ET-18-OMe was removed. In addition, we found significant changes in the sialic acid content and sialyltransferase activities in both conditions. Overall, these results clearly demonstrate that the uptake and retention of ET-18-OMe are responsible for the resistance to the invasion of malignant cells due to the altered sialylation of the cell surface glycoproteins in PHF.

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Section: Methodsmentioning

confidence: 99%

Edelfosine protects precultured heart fragments against the invasion of malignant cells through altered sialylation

Slambrouck

Steelant²,

Lopez³

et al. 2007

Oncol Rep

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“…The drawback of this method is that it can only be used for studies of relatively high-concentration edelfosine samples, such as pharmaceutical samples. The possibility of using capillary gas chromatography for the analysis of edelfosine was also evaluated [12,13]. This technique appears to be very attractive because of its intrinsically high selectivity and sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Comparative study of A HPLC–MS assay versus an UHPLC–MS/MS for anti-tumoral alkyl lysophospholipid edelfosine determination in both biological samples and in lipid nanoparticulate systems

Mendoza

Campanero

Mollinedo

et al. 2009

Journal of Chromatography B

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“…The retention and elution characteristics of both compounds were studied on silica, poly(ethylene glycol)-coated silica, reversed phase materials and polymeric resins. This method can only be used for studies of relatively highconcentration edelfosine samples [19]. The possibility of using capillary gas chromatography for the analysis of edelfosine was also investigated [20,21].…”

Section: Introductionmentioning

confidence: 99%

Quantitative determination of the antitumor alkyl ether phospholipid edelfosine by reversed-phase liquid chromatography–electrospray mass spectrometry: application to cell uptake studies and characterization of drug delivery systems

Blanco-Prieto

Campanero

Mollinedo

2004

Journal of Chromatography B

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Edelfosine is a synthetic alkyl ether phospholipid that represents a promising class of antitumor agents. However, analytical methods to measure these type compounds are scarce. The lack of a reliable methodology to quantify edelfosine is a major problem in ongoing and scheduled preclinical and clinical trials with this drug. We evaluated the applicability of high-performance liquid chromatography-mass spectrometry to determine edelfosine in biological samples and polymeric delivery systems. Sample pre-treatment involved polymer precipitation or cell lysis with methanol. HPLC separation was performed on an Alltima RP18 narrow-bore column and edelfosine quantification was done by electrospray ionization mass spectrometry (ESI-MS) using positive ion mode and selected ion monitoring. Assays were linear in the tested range of 0.3-10 µg/ml. The limit of quantification was 0.3 ng/sample in both matrices, namely biological samples and polymeric delivery systems. The interassay precision ranging from 0.79 to 1.49%, with relative errors of -6.7% and 12.8%. Mean extraction recovery was 95.6%.HPLC-ESI-MS is a reliable system for edelfosine analysis and quantification in samples from different sources, combining advantages of full automation (rapidity, ease of use, no need of extensive extraction procedures) with high analytical performance and throughput.

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Capillary GC analysis of alkyl lysophospholipids after derivatization with trimethylsilylbromide

Cited by 6 publications

References 4 publications

Edelfosine protects precultured heart fragments against the invasion of malignant cells through altered sialylation

Edelfosine protects precultured heart fragments against the invasion of malignant cells through altered sialylation

Comparative study of A HPLC–MS assay versus an UHPLC–MS/MS for anti-tumoral alkyl lysophospholipid edelfosine determination in both biological samples and in lipid nanoparticulate systems

Quantitative determination of the antitumor alkyl ether phospholipid edelfosine by reversed-phase liquid chromatography–electrospray mass spectrometry: application to cell uptake studies and characterization of drug delivery systems

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