Evaluation of different liquid chromatography–electrospray mass spectrometry systems for the analysis of heterocyclic amines

Barceló-Barrachina, E.; Moyano, Encarnación; Puignou, L.; Galcerán, M.T.

doi:10.1016/j.chroma.2003.10.030

Cited by 48 publications

(40 citation statements)

References 38 publications

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“…Moreover, according to the signal 3Â the peak-topeak noise, the calculated detection limits ranged between 11 nM (harmalol) and 90 nM (harmaline). These detection limits are considerably better than those reported in the literature using HPLC with UV [9], fluorimetric [8] and chemiluminescent [8] detection, and similar to those obtained using MS detection [6]. Moreover, the ranges of linearity obtained using amperometric detection at CNT-CGE are much wider than those reported in the literature.…”

Section: Lc-ec Detection At the Cnt-gcesupporting

confidence: 67%

“…The HPLC separation and determination of the b-carboline alkaloids were carried out using an isochratic elution and a C 18 reversed-phase column [1,6,7]. The mobile phase employed consisted of methanol/acetonitrile À 0.05 mol L À1 HPO 2À 4 solution of pH 9.0 [9,26].…”

Section: Lc-ec Detection At the Cnt-gcementioning

confidence: 99%

“…However, chemical derivatization to produce volatile b-carbolines is needed [4]. Moreover, HPLC combined with MS [5,6], fluorimetric [1,7,8], UV [9] or chemiluminescent [8] detection has been also used for trace determination of underivatized analytes. In spite of their electroactivity, there is a lack of electrochemical methods for the detection and quantification of these compounds.…”

Section: Introductionmentioning

confidence: 99%

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Voltammetric Characterization and Amperometric Detection of β‐Carboline Alkaloids at Carbon Nanotube‐Modified Electrodes

et al. 2007

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The voltammetric behavior of b-carboline alkaloids at carbon nanotubes-modified glassy carbon electrodes, as well as their quantification by amperometric monitoring of the oxidation responses, are reported. Cyclic voltammograms of harmane, harmine, harmaline and harmalol at the CNT-GCE showed enhanced voltammetric responses with respect to those obtained at a bare GCE. The effect of pH on the oxidation i p and E p values obtained by differential pulse voltammetry was discussed, as well as their mechanistic aspects. Hydrodynamic voltammogram of these compounds led us to select a potential value of þ 900 mV for their amperometric detection. Under these conditions, no fouling of the modified electrode surface is evidenced. The HPLC separation and determination of b-carboline alkaloids were carried out using a mobile phase consisting ofsolution of pH 9.0. Detection limits ranging between 28 nM (harmalol) and 88 nM (harmaline) were achieved. The usefulness of the chromatographic method with amperometric detection at the CNT-GCE was demonstrated by analyzing soy seeds samples spiked with harmaline, harmane and harmine at a 0.25 mg/g level.

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Section: Lc-ec Detection At the Cnt-gcesupporting

confidence: 67%

Section: Lc-ec Detection At the Cnt-gcementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Voltammetric Characterization and Amperometric Detection of β‐Carboline Alkaloids at Carbon Nanotube‐Modified Electrodes

et al. 2007

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“…The gradient elution program and the MS conditions were as previously described [30]. Normalized collision energies (NCE), time schedule and MS/MS parameters are given in Table 1.…”

Section: Analytical Conditionsmentioning

confidence: 99%

Quantification of heterocyclic amines from thermally processed meats selected from a small‐scale population‐based study

Busquets

Mitjans

Puignou

et al. 2008

Molecular Nutrition Food Res

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Heterocyclic amines (HAs) are potent mutagens that form at high temperatures in cooked, protein-rich food. Due to their frequent intake, these compounds are considered a risk factor for human cancer. Cooking conditions and eating habits strongly influence the level of HA exposure. Thus, it is difficult to assess the intake of HAs in a large population. Food-frequency questionnaires (FFQs), designed to provide data on parameters that affect HA formation, were used to survey a small population (459 persons) from Barcelona (NE Spain). Subsequently, the most-consumed food items named were cooked according to the preferences of the population surveyed and analyzed for HAs using SPE and LC-MS/MS. In the population studied, the estimated intake via consumption of 13 meat dishes was 285.6 ng of mutagenic HAs per capita and day. PhIP (2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine) was the HA to which the population was most exposed, mainly from fried chicken and griddled beef. When the co-mutagens norharman and harman are included, the mean daily intake of HAs rises to 475.6 ng per capita and day. A novel putative DMIP regioisomer was detected in the cooked meats, which was analyzed in the present study by multistage MS

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“…For all tested compounds and instrumental setups, linear response was observed from the LOQ up to 1 ppm, implying a linear range two orders of magnitude larger for the triple quadrupole instrument, because of its lower LOD. The method was applied for HAA determination in lyophilized beef extract 82,199 . A similar study was carried out by HPLC with multiple ion detection-APCI-MS, which showed high sensitivity on measuring the intensities of the [M + H] + ions.…”

Section: B Liquid Chromatography 1 Mass Spectrometric Detectionmentioning

confidence: 99%

Analytical Aspects of Aromatic Amines

Zabicky

2009

Patai's Chemistry of Functional Groups

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Evaluation of different liquid chromatography–electrospray mass spectrometry systems for the analysis of heterocyclic amines

Cited by 48 publications

References 38 publications

Voltammetric Characterization and Amperometric Detection of β‐Carboline Alkaloids at Carbon Nanotube‐Modified Electrodes

Voltammetric Characterization and Amperometric Detection of β‐Carboline Alkaloids at Carbon Nanotube‐Modified Electrodes

Quantification of heterocyclic amines from thermally processed meats selected from a small‐scale population‐based study

Analytical Aspects of Aromatic Amines

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