Evaluation of different extraction procedures for the quantification of seven cannabinoids in cannabis-based edibles by the use of LC-MS

Christodoulou, Marios C.; Christou, Atalanti; Stavrou, Ioannis J.; Kapnissi‐Christodoulou, Constantina P.

doi:10.1016/j.jfca.2022.104915

Cited by 12 publications

(15 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ultrasound-assisted extraction (UAE) is a technique that has been employed to extract cannabinoids from beverage and hemp tea samples . UAE utilizes ultrasonic waves to create rapid pressure changes in the solvent, which facilitate the breakdown of cell walls in the sample and enhance the release of cannabinoids.…”

Section: Cannabinoid Sample Preparation and Extraction Techniquementioning

confidence: 99%

“…UAE utilizes ultrasonic waves to create rapid pressure changes in the solvent, which facilitate the breakdown of cell walls in the sample and enhance the release of cannabinoids. Research by Christodoulou suggests that the most optimal UAE conditions involve using an ultrasonic bath operating at a frequency of 50–60 Hz and a maximum input power of up to 115 W . Implementing pulsed UAE (PUAE), characterized by a 30 min pulsing period followed by a 10 min rest interval, has been identified as the superior extraction approach.…”

Section: Cannabinoid Sample Preparation and Extraction Techniquementioning

confidence: 99%

“…Research has shown that ultrasonic-assisted extraction (UAE) yields higher extraction recoveries for solid cannabis samples. The Soxhlet extraction method is also used for extracting cannabinoids from solid matrices such as chocolate, hemp seeds, and hemp tea. ,, The efficiency of Soxhlet extraction relies on a longer extraction period with multiple cycles to increase the concentration of extractable cannabinoids.…”

Section: Cannabinoid Sample Preparation and Extraction Techniquementioning

confidence: 99%

“…Another methods comparison test was conducted by Christodoulou et al in 2023 regarding the efficiency of cannabinoid extraction by UAE and SPE . Their findings indicated that the UAE generally demonstrated a higher recovery rate for cannabinoids compared with SPE.…”

Section: Applications Of Extraction and Analytical Techniques To Edib...mentioning

confidence: 99%

See 3 more Smart Citations

Recent Advanced Methods for Extracting and Analyzing Cannabinoids from Cannabis-Infused Edibles and Detecting Hemp-Derived Contaminants in Food (2013–2023): A Comprehensive Review

Yang,

Sun

2024

J. Agric. Food Chem.

View full text Add to dashboard Cite

Section: Cannabinoid Sample Preparation and Extraction Techniquementioning

confidence: 99%

Section: Cannabinoid Sample Preparation and Extraction Techniquementioning

confidence: 99%

Section: Cannabinoid Sample Preparation and Extraction Techniquementioning

confidence: 99%

Section: Applications Of Extraction and Analytical Techniques To Edib...mentioning

confidence: 99%

See 2 more Smart Citations

Recent Advanced Methods for Extracting and Analyzing Cannabinoids from Cannabis-Infused Edibles and Detecting Hemp-Derived Contaminants in Food (2013–2023): A Comprehensive Review

Yang,

Sun

2024

J. Agric. Food Chem.

View full text Add to dashboard Cite

“…IR obtains characteristic substituents through the vibration of different chemical bonds. LC/GC obtains the basic planar structure of the compound by comparing the retention time of the chromatography with the standard, and MS can obtain the structure by multi‐level fragment ion peak analysis (Christodoulou et al, 2023). All the above methods can achieve high‐throughput and high‐sensitivity screening.…”

Section: Identification Of Plane Structurementioning

confidence: 99%

Recent advances on the structure identification of components in food

Zhao

Tian

2022

eFood

View full text Add to dashboard Cite

With the rapid development of food industry, people pay more and more attention to the health benefits of food‐active molecules. The active function of food molecules depends on the chemical structure. So how to determine the chemical composition of food becomes very important. Based on this, this paper mainly expounds the structural analysis methods and specific processes of food chemical components, as well as the difficulties encountered in the determination of structure, and summarizes the latest research trends of food structural analysis. The purpose is to let more scholars understand the structure analysis process of the chemical substance basis of food, and provide a reference for the healthy development of food.

show abstract

Optimization of microwave‐assisted extraction for quantification of cannabinoids in hemp tea by liquid chromatography‐mass spectrometry

Christodoulou,

Gonzalez‐Serrano,

Christou

et al. 2024

Separation Science Plus

Self Cite

View full text Add to dashboard Cite

Microwave‐assisted extraction (MAE) of cannabinoids from hemp tea was optimized, for the first, using response surface methodology. The effect of temperature (50, 65, and 80°C), irradiation time (4, 7, and 10 min), and solvent‐to‐solid ratio (20, 30, and 40 mL of methanol/g of hemp tea) on cannabinoid extractability were investigated. The concentrations of five cannabinoids, namely Δ9‐tetrahydrocannabinol (Δ9‐THC), cannabidiol (CBD), cannabigerol (CBG), cannabichromene (CBC), and cannabinol (CBN), were selected as response variables. For the quantitative analysis, a liquid chromatography‐mass spectrometry method was developed and validated. The proposed analytical approach demonstrated satisfactory performance characteristics in terms of linearity (R2 ≥ 0.9998), precision (intra‐day: 1.99%–5.97% relative standard deviation [%RSD], inter‐day: 1.95%–6.08%RSD), sensitivity (limit of detection: 1.35–2.36 ng/g, limit of quantification: 4.05–7.08 ng/g) and carry‐over effect (signals ≤ 5.03%), with all cannabinoids eluting within 6 min. For comparison purposes, soxhlet extraction, ultrasound‐assisted extraction (UAE), and conventional‐stirring extraction were additionally performed. MAE proved to be a more effective technique for the extraction of CBD and CBN, while UAE managed to extract Δ9‐THC, CBG, and CBC at higher concentration levels.

show abstract

Evaluation of different extraction procedures for the quantification of seven cannabinoids in cannabis-based edibles by the use of LC-MS

Cited by 12 publications

References 32 publications

Recent Advanced Methods for Extracting and Analyzing Cannabinoids from Cannabis-Infused Edibles and Detecting Hemp-Derived Contaminants in Food (2013–2023): A Comprehensive Review

Recent Advanced Methods for Extracting and Analyzing Cannabinoids from Cannabis-Infused Edibles and Detecting Hemp-Derived Contaminants in Food (2013–2023): A Comprehensive Review

Recent advances on the structure identification of components in food

Optimization of microwave‐assisted extraction for quantification of cannabinoids in hemp tea by liquid chromatography‐mass spectrometry

Contact Info

Product

Resources

About