Aim: The aim of this study was to evaluate the effect of desensitizer, temporary cements, dentin cleaning methods on shear bond strength (SBS) of self-etch and self-adhesive resin cements to dentin. Materials and Methods: Four hundred eighty buccal or lingual/palatine coronal portions of human molar teeth were mounted in the acrylic blocks. Teeth surfaces were prepared until the dentin was exposed. Specimens were divided into four groups. One group separated as control and three groups of teeth were treated with a desensitizing agent containing calcium-phosphate desensitizer (Teethmate Desensitizer, TMD), resin-based self-reinforcing monomer (Shield Force Plus, SFP) and 2-hydroxyethyl methacrylate and glutaraldehyde (Gluma Desensitizer). Two different temporary cement containing calcium hydroxide (Dycall) and zinc oxide based (Rely X Temp NE) temporary cement were applied to the dentin surfaces. Temporary cement was removed with one of three cleansing techniques: 2% chlorhexidine digluconate and glass particles containing agent (Consepsis Scrub), cleansing bur (OptiClean), and ıntra/extracoronal rotary brush (Starbrush). All groups were further divided into two cementation subgroups (Panavia V5 and Panavia SA). The SBS (MPa) was measured using a universal testing machine at 0.5 mm/min crosshead speed. The data were analyzed statistically with a four-way analysis of variance and Tukey’s Honest Significant Difference test ( α = 0.05). Results: Although SFP showed higher SBS, there was no statistically significant difference between other groups ( P > .05). There were no significant differences between temporary cements ( P > .05). OptiClean rotary bur showed higher mean SBS values in all groups. The dual-polymerized Panavia V5 showed the highest SBS values in all groups ( P <.05). Conclusion: Cleaning of dentin surface from temporary cement remnants with a silicon bur and dual-cure resin cement showed the highest bond strength values.