Evaluation of different chemical methods for cros-linking collagen gel, films and sponges

Rault, I.; Frei, V.; Herbage, D.; Abdul-Malak, Nabil; Huc, A.

doi:10.1007/bf00119733

Cited by 198 publications

(150 citation statements)

References 21 publications

(25 reference statements)

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“…Over the years, a number of cross-linking approaches (chemical, physical and biological) have been investigated (30); however, at present, there is not a commonly accepted ideal cross-linking treatment for collagen-derived bio-prosthesis. It has been recently reported that chemical stabilization through bifunctional agents (as epoxy compounds) leads to scaffolds that are characterized by thermal, structural, physical, and mechanical properties similar to those of native tissues (31).…”

Section: Discussionmentioning

confidence: 99%

Decellularized bovine reinforced vessels for small-diameter tissue-engineered vascular grafts

Grandi

Baiguera²,

Martorina³

et al. 2011

Int J Mol Med

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Abstract. The aim of the present study was to investigate the influence of a decellularization protocol on the structure and the mechanical behavior of small-diameter (<6 mm) tibial calf arteries and veins. Calf vessels were decellularized by a detergent-enzymatic method (DEM), partially hydrolyzed with trypsin and subsequently cross-linked using poly(ethylene glycol) diglycidyl ether. Our results showed that i) the DEM can be considered a simple and valuable procedure for the preparation of complete acellular arteries and veins able to preserve a high degree of collagen and elastic fibers, and ii) poly(ethylene glycol) diglycidyl ether cross-linking treatment provides appropriate mechanical reinforcement of blood vessels. Histologically, the decellularized vessels were obtained employing the detergent-enzymatic procedure and their native extracellular matrix histoarchitecture and components remained well preserved. Moreover, the decellularization protocol can be considered an effective method to remove HLA class I antigen expression from small-diameter tibial calf arteries and veins. Cytocompatibility of decellularized cross-linked vessels was evaluated by endothelial and smooth muscle cell seeding on luminal and adventitial vessel surfaces, respectively.

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Section: Discussionmentioning

confidence: 99%

Decellularized bovine reinforced vessels for small-diameter tissue-engineered vascular grafts

Grandi

Baiguera²,

Martorina³

et al. 2011

Int J Mol Med

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“…The greater amount of glutamic acid and aspartic acid in BF collagen could explain the different physical-chemical properties of the HACSMs composed of higher BF collagen ratios, for example reduction in pore size and swelling ratio, enhancement in tensile strength, resistance to collagenase, and thermal stability. The EDC cross-linking system is frequently used in the porous collagen materials [5,9,11,14,15], which has shown better biocompatibility than glutaraldehyde (GA) [9]. In principle, the EDC cross-linking takes place by the reaction between carboxyl groups of glutamic acid and aspartic acid and generated stable amide bonds [9,11,[13][14][15]24,25].…”

Section: Discussionmentioning

confidence: 99%

Studies of Novel Hyaluronic Acid-collagen Sponge Materials Composed of Two Different Species of Type I Collagen

Lin

Liu

2006

J Biomater Appl

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ABSTRACT:The authors have developed novel hyaluronic acid (HA)-collagen sponge materials (HACSMs) composed of various ratios of bird feet (BF) and pig skin (PS) collagen that are fabricated employing a combination of freezing, lyophilizing, and 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) crosslinking methods. Morphology, swelling ratio, resistance to collagenase, thermal stability, tensile strength, and free amine index are determined to evaluate the physical-chemical properties of various HACSMs. Different BF: PS ratios directly vary with the physical-chemical properties of HACSMs and control their biodegradability for multiple uses. Resistance to collagenase, thermal stability, and tensile strength of HACSMs increases as the ratio of BF collagen increases. On the contrary, the higher swelling ratio, free amine index, and pore size occur in materials composed of higher ratios of PS collagen. A linear relationship between the decreased ratio of PS collagen and the increase in tensile strength and biostability are observed. The materials of B4P1HA (BF : PS : HA ¼ 4 : 1 : 0.2) exhibit the highest value of tensile strength, but no significant difference exists between B4P1HA and B5P0HA (BF : PS : HA ¼ 5 : 0 : 0.2). These phenomena should be closely related to the BF collagen which contains a higher amount of carboxyl groups of glutamic or aspartic acid residues and forms more amine bonds under EDC cross-linking *Author to whom correspondence should be addressed. E-mail: nitrite.tw@gmail.com when compared to PS collagen. However, these results suggest that the B4P1HA and B5P0HA materials should be produced according to highest bio-stability and mechanical strength and, furthermore they may be suitable for artificial skin or drug delivery applications. JOURNAL OF BIOMATERIALS APPLICATIONS

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“…The TNP-Gly to be modified, on the other hand, carried amino groups. Therefore, EDC/NHS was chosen to serve as the activator for the coupling reaction [30][31][32][33]. As shown in Figure 4, consulting the test results of different EDC/NHS ratios, the ratio of EDC: NHS = 4:1 was chosen for further experiments.…”

Section: Chosen Of Chip Activatormentioning

confidence: 99%

Modification and characterization of an aptamer-based surface plasmon resonance sensor chip

et al. 2017

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Abstract. Recently, aptamer-based surface plasmon resonance (SPR) sensors have become increasingly popular due to their high specificity, high sensitivity, real-time detection capabilities, and label-free features. The core component of an aptamer-based SPR sensor is a chip. This paper presents the modification steps and the characterization results of a sensor chip for the construction of a 2, 4, 6-trinitrotoluene-targeted, aptamer-based, SPR sensor. After cleaning the aptamer-based SPR sensor chip, polyethylene glycol (PEG) with functional thiol groups at one end was added to the chip surface by Au-S covalent bonds to form a selfassembled film. Then, the carboxyl groups at the other end of PEG and the carboxyl groups of trinitrophenyl-glycine (TNP-Gly) were activated and connected via ethylenediamine (EDA). This effectively completed the chip's modification. During the modification process, relevant experimental conditions were optimized. The chip's surface elements, as well as their chemical states, were characterized by X-ray photoelectron spectroscopy (XPS). The results, outlined in the following study, demonstrate that this modification of an aptamer-based SPR sensor chip adhered to normative expectations. Thus, the modification process proposed here establishes an important foundation for subsequent study of TNT detection.

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Evaluation of different chemical methods for cros-linking collagen gel, films and sponges

Cited by 198 publications

References 21 publications

Decellularized bovine reinforced vessels for small-diameter tissue-engineered vascular grafts

Decellularized bovine reinforced vessels for small-diameter tissue-engineered vascular grafts

Studies of Novel Hyaluronic Acid-collagen Sponge Materials Composed of Two Different Species of Type I Collagen

Modification and characterization of an aptamer-based surface plasmon resonance sensor chip

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