Evaluation of different analytical strategies for the quantification of sulfur-containing biomolecules by HPLC-ICP-MS: Application to the characterisation of 34S-labelled yeast

Determination of low‐level (sub‐microgram) sulfur concentrations by isotope dilution multi‐collector inductively couple plasma mass spectrometry using a 33S spike and internal normalization for mass bias correction

“…Martínez‐Sierra et al . were the first to employ a 33 S‐enriched spike for isotope dilution; however, they used the sample‐standard bracketing approach for the correction of mass bias.…”

“…Martínez-Sierra et al [22,23] were the first to employ a 33 S-enriched spike for isotope dilution; however, they used the samplestandard bracketing approach for the correction of mass bias. In this paper, we present results for a 33 S-enriched spike Isotope Dilution Multi-Collector Inductively Couple Plasma Mass Spectrometry (ID-MC-ICP-MS) method that uses internal normalization for the correction of mass bias.…”

In vivo synthesized 34S enriched amino acid standards for species specific isotope dilution of proteins

“…7 The most evident direct targeting approach would involve quantication of intact proteins by sulfur using size exclusion chromatography and ICP-MS detection. 8,9 As a prerequisite, the amino acid sequence and hence the number of sulfur containing amino acids has to be known. Size exclusion chromatography is perfectly suited to separate inorganic and low molecular weight organic impurities from proteins.…”

Quantification of pharmaceutical peptides in human plasma by LC-ICP-MS sulfur detection

“…23 The technique and the equations used in calculations have been presented in a tutorial review by Rodriguez-Gonzáles. 24 Sulfur post column IDA has been applied in several studies regarding protein quantication by ICP-MS. 22,25,26 The number of quantitative studies of peptides or proteins in complex biological matrices using ICP-MS detection of sulfur is very limited. Konz et al developed a method for quantication of the peptide hepcidin in human urine by post column IDA 27 but, the majority of studies does not report on using IDA for quantication.…”