Evaluation of diagnostic methods for Candida albicans translocation in a mouse model: seminested polymerase chain reaction, blood culture, and serological assays

Uno, Kenji; Sugiura, Shigeki; Konishi, Mitsuru; Yasuda, Yasuki; Mikasa, Keiichi; Kita, Eisuke

doi:10.1007/s10156-007-0536-5

Cited by 5 publications

(2 citation statements)

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“…Therefore, better diagnostics are required for the detection of systemic Candida infection. Both mouse models of systemic C. albicans infection have been used to evaluate novel diagnostics before a clinical trial (Nichterlein et al, 2003;Uno et al, 2007). Evaluation of new diagnostics in a host where systemic infection can be reliably induced demonstrated that serological tests for Candida mannan and b-glucan were more sensitive than nested PCR and blood culture for the prediction of systemic infection in the mouse (Uno et al, 2007).…”

Section: The Role Of Mouse Infection Models In the Development Of Newmentioning

confidence: 99%

“…Both mouse models of systemic C. albicans infection have been used to evaluate novel diagnostics before a clinical trial (Nichterlein et al, 2003;Uno et al, 2007). Evaluation of new diagnostics in a host where systemic infection can be reliably induced demonstrated that serological tests for Candida mannan and b-glucan were more sensitive than nested PCR and blood culture for the prediction of systemic infection in the mouse (Uno et al, 2007). These tests have been further developed for clinical use, for example Platelia s Candida mannan antigen sandwich enzyme-linked immunosorbent assay (Bio-rad Laboratories) and Fungitell s assay (Associates of Cape Cod Inc.).…”

Section: The Role Of Mouse Infection Models In the Development Of Newmentioning

confidence: 99%

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The contribution of mouse models to our understanding of systemic candidiasis

Szabó

MacCallum

2011

FEMS Microbiology Letters

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Some Candida species are common commensals, which can become opportunistic pathogens in susceptible hosts. In severely ill patients, Candida species, particularly Candida albicans, can cause life-threatening systemic infections. These infections are difficult to diagnose, as symptoms are similar to those of systemic bacterial infections. These difficulties can lead to delays in initiation in antifungal therapy, which contributes to the high mortality rates (> 40%) associated with these infections. In order to investigate systemic Candida infection, mouse models have been developed that mimic human disease, the most common being the intravenous infection model and the gastrointestinal colonization and dissemination model. This review discusses the two models and the contributions that they have made to our understanding of fungal virulence, host response to infection and the development of novel antifungal therapies and diagnostics.

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Section: The Role Of Mouse Infection Models In the Development Of Newmentioning

confidence: 99%