Utilidad de los marcadores biológicos en el diagnóstico de la candidiasis invasora

Pontón, José

doi:10.1016/s1130-1406(09)70003-8

Cited by 19 publications

(8 citation statements)

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“…Invasive candidiasis diagnosis is difficult because signs and symptoms are unspecific, as well as due to the fact that these opportunistic yeasts dwell mainly in mucosae in humans 11 . Clinical laboratory conventional procedures to diagnose invasive candidiasis include morphological and biochemical studies, such as smear, culture, histopathology, serology and, sometimes, the use of specific fluorescent antibodies 1,3,[12][13][14] .…”

Section: Introductionmentioning

confidence: 99%

“…However, sensitivity and specificity of these methods are low. In neutropenic patients, yeasts are detected by culture or histopathology approximately 120-140 days after the detection of this predisposing factor, which results in late specific therapy 11 . For some authors, culture is the reference method for invasive candidiasis diagnosis 15 .…”

Section: Introductionmentioning

confidence: 99%

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Molecular detection of Candida species from hospitalized patients’ specimens

Camacho-Cardoso

Martínez-Rivera

Manzano-Gayosso

et al. 2023

GMM

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Objective: To identify the most frequent Candida species in specimens from patients hospitalized in different medical centers of Mexico City, with suspected fungal infection. Methods: Specimens were grown on Sabouraud dextrose agar at 28°C for 72 h. In addition, DNA was extracted. Isolates were grown on CHROMagar Candida™, at 37°C for 48 h. The molecular identification was performed by polymerase chain reaction (PCR) using primers specific for four species. Results: Eighty one specimens were processed and included: bronchial lavage, pleural, cerebrospinal, peritoneal, ascites and bile fluids; blood, sputum, bone marrow, oro-tracheal cannula and ganglion. By culture, 30 samples (37%) were positive, and by PCR, 41 (50.6%). By PCR, the frequency of species was: Candida albicans 82.9%, Candida tropicalis 31.7%, Candida glabrata 24.4%, and Candida parapsilosis 4.9%. In 34.1% of specimens a species mixture was detected suggesting a co-infection: Two species in five specimens (C. albicans-C tropicalis and C. albicans-C glabrata), and three species in three specimens (C. albicans-C. glabrata-C. tropicalis). Conclusions: The PCR is an useful tool for detection the most common Candida species causing infection in hospitalized patients, it avoids the requirement of culture weather we start from clinical specimen and it favors the early diagnosis of invasive candidiasis.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular detection of Candida species from hospitalized patients’ specimens

Camacho-Cardoso

Martínez-Rivera

Manzano-Gayosso

et al. 2023

GMM

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“…Se solicitaron especímenes biológicos a diferentes centros hospitalarios de la ciudad de México, provenientes de pacientes hospitalizados, con uno o más factores de oportunismo, sospecha clínica de micosis invasiva o diagnóstico no determinado asociado a fiebre de origen desconocido. Se recibieron y procesaron 81 muestras del Laboratorio Central correspondiente: 11 Todas las muestras clínicas fueron cultivadas en agar dextrosa Sabouraud (ADS) sin antibióticos, incubadas a 28 °C por 72 horas. Se registró el número de unidades formadoras de colonias (UFC).…”

Section: Métodosunclassified

“…El diagnóstico de la candidiasis invasiva es difícil debido a que los signos y síntomas son inespecíficos, así como al hecho de que estas levaduras oportunistas son comensales principalmente de las mucosas en el humano 11 . En el laboratorio clínico, los procedimientos convencionales para realizar el diagnóstico de la candidiasis invasiva incluyen estudios morfológicos y bioquímicos, como el frotis, el cultivo, la histopatología, la serología y, en ocasiones, el uso de anticuerpos fluorescentes específicos 1,3,[12][13][14] .…”

Section: Introductionunclassified

“…Sin embargo, la sensibilidad y la especificidad de estos métodos son bajas. En los pacientes neutropénicos se detectan levaduras por cultivo o por histopatología aproximadamente a los 120-140 días posteriores a la detección de este factor predisponente, lo cual induce una terapia específica tardía 11 . Para algunos autores, el cultivo es el método de referencia para el diagnóstico de la candidiasis invasiva 15 .…”

Section: Introductionunclassified

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Detección molecular de especies de Candida en especímenes de pacientes hospitalizados

Camacho-Cardoso

Martínez-Rivera

Manzano-Gayosso

et al. 2017

GMM

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Evaluation of multiplex PCR in first episodes of febrile neutropenia as a tool to improve early yeast diagnosis in leukemic/preleukemic patients

Teixeira

Silva

Matias

et al. 2014

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In febrile neutropenic onco-hematological patients, delayed microbiological diagnosis leads to an increase in morbidity and mortality. Identification of the microorganism changes antibiotic therapy in more than half of cases; however, in only 20-30 % of such cases pathogen isolation is achieved. This study evaluates the frequency of fungus infection and its etiology in onco-hematological patients with febrile neutropenia utilizing blood cultures and non-commercial multiplex polymerase chain reaction (MT-PCR) primers. Fifty-three febrile neutropenia episodes in 35 onco-hematological patients were observed, and the results for the first unique 30 episodes are presented. Blood cultures were positive for Candida tropicalis (one case), gram-positive bacteria (two cases), and gram-negative bacteria (four cases), showing a 23.3 % microbiological isolation rate. Multiplex-PCR pan-fungal sequence was positive in 18 cases (60 %), and further sequencing identified fugal pathogens in 11 cases (Candida glabrata and Candida parapsilosis being the most common). MT-PCR pan-fungal sequence amplification was detected in 13 of 16 patients that later received antifungal treatment for clinical reasons only, while positivity was found in 5 out of 14 patients that did not receive antifungal treatment (p = 0.02). These results show that performing in-house non-commercial MT-PCR is feasible and may provide additional information about fungal infection without the need to wait for culture results. Further research is necessary to incorporate this technology into the decision-making process.

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Utilidad de los marcadores biológicos en el diagnóstico de la candidiasis invasora

Cited by 19 publications

References 50 publications

Molecular detection of Candida species from hospitalized patients’ specimens

Molecular detection of Candida species from hospitalized patients’ specimens

Detección molecular de especies de Candida en especímenes de pacientes hospitalizados

Evaluation of multiplex PCR in first episodes of febrile neutropenia as a tool to improve early yeast diagnosis in leukemic/preleukemic patients

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