Evaluation of Diagnostic Assays for Neonatal and Infantile Chlamydial Infections.

Abstract

Several diagnostic assays for neonatal and infantile chlamydial infections, isolation with tissue culture, antigen detection by enzyme immunoassay (ETA) (IDEIA Chlamydia test), a nonisotopic DNA probe (Gen-Probe PACE 2 assay), serum IgM antibody detection by ETA (SeroELISA Chlamydia TRUE IgM), and polymerase chain reaction (PCR), were evaluated. Of 210 clinical specimens (170 nasopharyngeal and 40 conjunctival swabs) from 53 neonates and 102 infants with respiratory insufficiency and respiratory tract infections which were suspected to be associated chlamydial infection, chlamydial antigens were detected in 30 by IDEIA Chlamydia. Of these 3Q samples, C. trachomatis was isolated from 27 specimens. Samples from 15 neonates and 6 infants were culture-positive and IDEIA Chlamydia-positive. Of 30 samples, 27 were tested with PCR and 8 with DNA probe. Twenty-three of 27 specimens were positive with PCR, while only one specimen was positive with DNA probe. ETA can be used for the diagnosis and screening of neonatal and infantile chlamydial infections.

Chlamydia trachomatisinfection in early neonatal period

“…[7,8] Maternal serum, infantile serum and cord blood samples were obtained for standard microimmunofluorescence (MIF) assay to detect IgG and IgM antibodies against C. trachomatis . [2]…”

Unclassified serovars of Chlamydia trachomatis isolated from Japanese infants

“…DEAE‐dextran‐ and cycloheximide‐treated HeLa 229 cells were used to isolate C. trachomatis from the harvested culture media as reported previously [8]. A commercially available EIA kit (IDEIA CHLAMYDIA, Dako Diagnostics Co., USA) was also used to detect genus‐specific chlamydial antigens in lipopolysaccharide (LPS) epitope of the cell wall [9].…”

“…Comparison of the KFLP profiles of each clinical isolate with those of the reference strains was performed to assign a serovar from the genotype of each clinical isolate. isolation and antigen detection of C. rrachomaris IIEAI-dextran-and cycloheximide-treated HeLa 229 cells were used to isolate C. tracliomatis from the harvested culture media as reported previously [8]. A commercially available EIA kit (IDEIA CHLAMYDIA, Dako Diagnostics Co., USA) was also used to detect genus-specific chlamydia1 antigens in lipopolysaccharide (LPS) epitope of the cell wall 191.…”

Current problems of perinatal Chlamydia trachomatisinfections

“…Early diagnosis and appropriate treatment of chlamydial infections may reduce these complications. [43,44] Although further studies in large number of populations are definitely necessary, detection of serum IgG and IgA antibodies to C. trachomatis during late stage of pregnancy is considered to permit more laboratories to diagnose perinatal chlamydial infections and also to be useful for the screening of infection.…”