2014
DOI: 10.1002/jat.3010
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Evaluation of developmental toxicity using undifferentiated human embryonic stem cells

Abstract: An embryonic stem cell test (EST) has been developed to evaluate the embryotoxic potential of chemicals with an in vitro system. In the present study, novel methods to screen toxic chemicals during the developmental process were evaluated using undifferentiated human embryonic stem (hES) cells. By using surface marker antigens (SSEA-4, TRA-1-60 and TRA-1-81), we confirmed undifferentiated conditions of the used hES cells by immunocytochemistry. We assessed the developmental toxicity of embryotoxic chemicals, 5… Show more

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Cited by 25 publications
(12 citation statements)
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References 63 publications
(76 reference statements)
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“…This in vitro differentiation system provides unique tools for the study of early cardiovascular development [5], drug screening [6,7], cytotoxicity testing [8,9], and cardiac regenerative therapy [10,11]. However, the physiological and pharmacological properties of hESCs and CVPCs, such as the Ca 2+ signaling and the response to various modulators, are largely unknown.…”
Section: Introductionmentioning
confidence: 99%
“…This in vitro differentiation system provides unique tools for the study of early cardiovascular development [5], drug screening [6,7], cytotoxicity testing [8,9], and cardiac regenerative therapy [10,11]. However, the physiological and pharmacological properties of hESCs and CVPCs, such as the Ca 2+ signaling and the response to various modulators, are largely unknown.…”
Section: Introductionmentioning
confidence: 99%
“…The gene expression profiles of mouse and human ES cells have been proven to be distinct in, for example, several differentiation markers (Liu, Yin, & Faiola, 2017;Tandon & Jyoti, 2012;Wobus & Loser, 2011), which may lead to completely different responses to the test chemicals in molecular approaches (Yamane et al, 2016). Several recent studies have thus investigated the potential application of human embryonic stem cells (hESCs), associated with a variety of molecular endpoints, to predict the developmental toxicity in vitro (Ehashi, Suzuki, Ando, Sumida, & Saito, 2014;Hong & Jeung, 2013;Jung et al, 2014;Sandstrom et al, 2017). However, in addition to ethical issues, using hESCs for drug screening not only requires complex culturing conditions but also is costly and difficult to implement in the HT manner (Fang, Zhi, Yu, Lynch, & Jia, 2018;Rezvanfar, Hodjat, & Abdollahi, 2016).…”
Section: Discussionmentioning
confidence: 99%
“…The abnormal expression of MCP2 will directly lead to embryonic diapauses or might affect the embryo implantation and growth, indirectly causing abortion. Therefore, the study of the changes in expression of MCP2 has important significance in illustrating the pathogenesis of habitual abortion (Park et al, 2014;Pérez-Garijo and Steller, 2014;Hsuuw and Chan, 2015;Jung et al, 2015). TLR4 is one of the important human innate immune receptors.…”
Section: Discussionmentioning
confidence: 99%