Evaluation of dermal collagen stained with picrosirius red and examined under polarized light microscopy

Coelho, Polyana Galvão Bernardes; Souza, Maria Verônica de; Conceição, Lissandro Gonçalves; Vilória, Marlene Isabel Vargas; Bedoya, Sirley Adriana Ortiz

doi:10.1590/abd1806-4841.20187544

Cited by 39 publications

(28 citation statements)

References 8 publications

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“…The stained sections were analyzed using an Olympus IX73 inverted microscope (Olympus Corporation; magnification, ×200 or ×400) with a linear polarizer. To avoid missing any details, the filter was tilted to an angle between 0 and 90 until the birefringence became evident and the background became completely black; the focus was then corrected once more ( 25 ). The halogen lamp intensity and exposure time were constant within each image.…”

Section: Methodsmentioning

confidence: 99%

Prognostic significance of abnormal matrix collagen remodeling in colorectal cancer based on histologic and bioinformatics analysis

Liang

Huang

et al. 2020

Oncol Rep

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As the major component of the tumor matrix, collagen greatly influences tumor invasion and prognosis. The present study compared the remodeling of collagen and collagenase in 56 patients with colorectal cancer (CRC) using Sirius red stain and immunohistochemistry, exploring the relationship between collagen remodeling and the prognosis of CRC. Weak or strong changes in collagen fiber arrangement in birefringence were observed. With the exception of a higher density, weak changes equated to a similar arrangement in normal collagen, while strong changes facilitated cross-linking into bundles. Compared with normal tissues, collagen I (COL I) and III (COL III) deposition was significantly increased in CRC tissues, and was positively correlated with the metastasis status. In tissues without distant metastasis, collagen IV (COL IV) levels were higher than that in normal tissues, while in tissues with distant metastasis, collagen IV expression was significantly lower. Furthermore, the expression of matrix metalloproteinase (MMP)-1, MMP-2, MMP-7, MMP-9 and lysyl oxidase-like 2 (LOXL2) was found to be elevated in the cancer stroma, which contributed to the hyperactive remodeling of collagen. The association between collagen-related genes and the occurrence and prognosis of CRC were analyzed using biometric databases. The results indicated that patients with upregulated expression of a combination of coding genes for collagen and collagenase exhibited poorer overall survival times. The coding genes COL1A1-2, COL3A1, COL4A3, COL4A6 and MMP2 may therefore be used as biomarkers to predict the prognosis of patients with CRC. Furthermore, the results of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis suggest that collagen may promote tumor development by activating platelets. Collectively, the abnormal collagen remodeling, including associated protein and coding genes is associated with the tumorigenesis and metastasis, affecting the prognosis of patients with CRC.

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Section: Methodsmentioning

confidence: 99%

Prognostic significance of abnormal matrix collagen remodeling in colorectal cancer based on histologic and bioinformatics analysis

Liang

Huang

et al. 2020

Oncol Rep

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“…Relatively inexpensive, the technique relies on the birefringent properties of collagen molecules. While the PSR stain alone does not selectively bind the collagen network, it becomes more specific than the other common collagen stains when combined with polarized light detection [ 9 , 11 ]. Under circularly polarized PSR microscopy, thin bundles of collagen type III appear green-yellow, whereas large bundles of tightly packed, well-organized and mature collagen type I molecules appear yellow-orange [ 9 ].…”

Section: Discussionmentioning

confidence: 99%

“…Under circularly polarized PSR microscopy, thin bundles of collagen type III appear green-yellow, whereas large bundles of tightly packed, well-organized and mature collagen type I molecules appear yellow-orange [ 9 ]. Use of circularly polarized PSR microscopy revealed bundles of yellow-orange collagen I [ 9 , 11 ] along the intrahepatic duct walls, indicating the presence of mature collagen type I, which provides both structural support and tensile strength [ 9 ]. By contrast, there was no accumulation of collagen I or collagen III along the renal cyst walls.…”

Section: Discussionmentioning

confidence: 99%

Collagen-driven remodeling of the intrahepatic duct wall in the PCK rat model of polycystic kidney disease-Caroli syndrome

Narayan¹,

Pellicano

et al. 2020

ceh

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Aim of the study Autosomal dominant polycystic kidney disease (ADPKD) is characterized by formation and expansion of cysts within the kidney. Caroli syndrome (CS) is characterized by cystic saccular dilatation of intrahepatic ducts. Kidney and liver images from a model of ADPKD-CS were evaluated to characterize remodeling of the cystically dilated intrahepatic duct wall and the renal cyst wall. Material and methods Archival digitized images from Masson’s trichrome-stained renal and Picrosirius red (PSR)-stained renal and hepatic cross-sections were sourced from the PCK rat model of ADPKD-CS, and age-matched Sprague-Dawley rats (wild-type). Cross-sectional areas and wall thicknesses of renal cysts and intrahepatic ducts were measured. Circularly polarized PSR microscopy was utilized to observe accumulation of collagen and identify its subtype. Results In the PCK rat model of ADPKD-CS, renal cysts were relatively thin-walled in comparison to intrahepatic ducts with renal cyst cross-sectional area to wall ratio 47-fold greater than the corresponding ratio in intrahepatic ducts. Increasing intrahepatic duct cross-sectional area was accompanied by a rapid and steep rise in wall thickness. There was a weak but significant direct correlation ( r = 0.49, p = 0.037) between renal cyst cross-sectional area and wall thickness. Circularly polarized Picrosirius red microscopy revealed collagen I accumulation within the walls of dilated intrahepatic ducts but not renal cysts. Conclusions These data suggest that unlike renal cysts, cystically dilated intrahepatic ducts undergo collagen-driven wall remodeling in the PCK rat.

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“…Adjacent sections were stained with Alcian blue and Picrosirius red and subsequently imaged under polarized light to visualize collagen fiber organization 26 . Birefringence of collagen fibers was then determined based on the intensity of individual pixels captured using polarized light microscopy 27 . To confirm effectiveness of the HFD, livers were harvested, fixed in 4% paraformaldehyde in PBS for 16 h at 4 °C, washed 3 times with PBS, and cryoprotected in 30% sucrose for 16 h at 4 °C.…”

Section: Methodsmentioning

confidence: 99%

Obesity alters the collagen organization and mechanical properties of murine cartilage

Collins

Newman

et al. 2021

Sci Rep

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Osteoarthritis is a debilitating disease characterized by cartilage degradation and altered cartilage mechanical properties. Furthermore, it is well established that obesity is a primary risk factor for osteoarthritis. The purpose of this study was to investigate the influence of obesity on the mechanical properties of murine knee cartilage. Two-month old wild type mice were fed either a normal diet or a high fat diet for 16 weeks. Atomic force microscopy-based nanoindentation was used to quantify the effective indentation modulus of medial femoral condyle cartilage. Osteoarthritis progression was graded using the OARSI system. Additionally, collagen organization was evaluated with picrosirius red staining imaged using polarized light microscopy. Significant differences between diet groups were assessed using t tests with p < 0.05. Following 16 weeks of a high fat diet, no significant differences in OARSI scoring were detected. However, we detected a significant difference in the effective indentation modulus between diet groups. The reduction in cartilage stiffness is likely the result of disrupted collagen organization in the superficial zone, as indicated by altered birefringence on polarized light microscopy. Collectively, these results suggest obesity is associated with changes in knee cartilage mechanical properties, which may be an early indicator of disease progression.

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Evaluation of dermal collagen stained with picrosirius red and examined under polarized light microscopy

Cited by 39 publications

References 8 publications

Prognostic significance of abnormal matrix collagen remodeling in colorectal cancer based on histologic and bioinformatics analysis

Prognostic significance of abnormal matrix collagen remodeling in colorectal cancer based on histologic and bioinformatics analysis

Collagen-driven remodeling of the intrahepatic duct wall in the PCK rat model of polycystic kidney disease-Caroli syndrome

Obesity alters the collagen organization and mechanical properties of murine cartilage

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