Evaluation of Dehydrated Human Amnion/Chorion Membrane as an Adhesion Barrier in Women Undergoing Robotic Laparoscopy

Dulemba, John; Mirzakhani, Pezhman

doi:10.4172/2161-0932.1000405

Cited by 6 publications

(4 citation statements)

References 12 publications

(15 reference statements)

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“…The clinical impact of amniotic membrane allografts as barriers has been demonstrated in cases to limit postoperative adhesion in abdominal, otolaryngologic, and laminectomy surgery and ocular injuries. [32][33][34][35][36][37] The processing and preservation of LHACM and DHACM produce allografts that function as membrane barriers. However, it is also critical to understand the impact of processing techniques on the biomolecules embedded within the allografts.…”

Section: Discussionmentioning

confidence: 99%

PURION^® processed human amnion chorion membrane allografts retain material and biological properties supportive of soft tissue repair

Moreno,

Massee,

Campbell

et al. 2024

J Biomater Appl

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The reparative properties of amniotic membrane allografts are well-suited for a broad spectrum of specialties. Further enhancement of their utility can be achieved by designing to the needs of each application through the development of novel processing techniques and tissue configurations. As such, this study evaluated the material characteristics and biological properties of two PURION® processed amniotic membrane products, a lyophilized human amnion, intermediate layer, and chorion membrane (LHACM) and a dehydrated human amnion, chorion membrane (DHACM). LHACM is thicker; therefore, its handling properties are ideal for deep, soft tissue deficits; whereas DHACM is more similar to a film-like overlay and may be used for shallow defects or surgical on-lays. Characterization of the similarities and differences between LHACM and DHACM was conducted through a series of in vitro and in vivo studies relevant to the healing cascade. Compositional analysis was performed through histological staining along with assessment of barrier membrane properties through equilibrium dialysis. In vitro cellular response was assessed in fibroblasts and endothelial cells using cell proliferation, migration, and metabolic assays. The in vivo cellular response was assessed in an athymic nude mouse subcutaneous implantation model. The results indicated the PURION® process preserved the native membrane structure, nonviable cells and collagen distributed in the individual layers of both products. Although, LHACM is thicker than DHACM, a similar composition of growth factors, cytokines, chemokines and proteases is retained and consequently elicit comparable in vitro and in vivo cellular responses. In culture, both treatments behaved as potent mitogens, chemoattractants and stimulants, which translated to the promotion of cellular infiltration, neocollagen deposition and angiogenesis in a murine model. PURION® processed LHACM and DHACM differ in physical properties but possess similar in vitro and in vivo activities highlighting the impact of processing method on the versatility of clinical use of amniotic membrane allografts.

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Section: Discussionmentioning

confidence: 99%

PURION^® processed human amnion chorion membrane allografts retain material and biological properties supportive of soft tissue repair

Moreno,

Massee,

Campbell

et al. 2024

J Biomater Appl

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“…The majority of these do not contain cells and are derived from the human placental membrane, animal tissue, or donated human dermis. Including noninfected and infected wound costs, the estimated cost of care for diabetic foot ulcers ranged from $6.2 billion to $18.7 billion, for venous leg ulcers the range was $0.7 billion to $1.5 billion, and for pressure ulcers the range was $3.9 billion to $22 billion [45,48,49].…”

Section: Discussionmentioning

confidence: 99%

Using of Amniotic Membrane Derivatives for the Treatment of Chronic Wounds

Schmiedova

Dembickaja

Kiselakova³

et al. 2021

Membranes

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Amniotic membrane grafts have some therapeutic potential for wounds healing. Early application of amniotic membrane turned out as beneficial in healing ulcers, burns, and dermal injuries. Since the second half of the 20th century, the autotransplants of amniotic/chorion tissue have been also used for the treatment of chronic neuropathic wounds, cornea surface injuries, pterygium and conjunctivochalasis, and dental and neurosurgical applications. The aim of this publication is to prepare a coherent overview of amniotic membrane derivatives use in the field of wound healing and also its efficacy. In total 60 publications and 39 posters from 2000–2020 were examined. In these examined publications of case studies with known study results was an assemblage of 1141 patients, and from this assemblage 977 were successfully cured. In case of posters, the assemblage is 570 patients and 513 successfully cured. From the investigated data it is clear that the treatment efficacy is very high—86% and 90%, respectively. Based on this information the use of the amniotic membrane for chronic wounds can be considered highly effective.

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“…Mrugala et al reported accelerated would healing after application of dHACM in five case reports in patients with chronic, nonhealing ulcers [9]. Dulemba et al observed no adhesion formation in 14 of 15 cases of women undergoing robotic laparoscopy [10]. Patel et al observed accelerated return of continence and potency in patients following nerve-sparing robot-assisted radical prostatectomy after the application of dHACM on the prostatic neurovascular bundle [11].…”

Section: Discussionmentioning

confidence: 99%

Novel Role of Dehydrated Human Amnion/Chorion Membrane in Healing of Denuded Ureter in Robot-Assisted Excision of Endometriosis

Hasan

Johnson

Siegert

et al. 2019

Case Reports in Obstetrics and Gynecology

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Ureteral injury is an uncommon but potentially morbid complication following any open or endoscopic pelvic procedure. Gynecologic surgeries alone make up 50 percent of nonurologic ureteral injuries leading to prolonged hospital stays, secondary interventions, and potential loss of renal function. The use of AmnioFix ® a processed dehydrated, immunologically privileged cellular amniotic membrane allograft has been well established in urologic and gynecologic procedures. These allografts contain human extracellular matrix components, growth factors, and cytokines that mediate inflammation and facilitate would healing. We report the first application of AmnioFix on a denuded ureter during a case of robotic-assisted excision of endometriosis. We include a literature review and discussion on the management outcomes of iatrogenic injury to the ureters.

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Evaluation of Dehydrated Human Amnion/Chorion Membrane as an Adhesion Barrier in Women Undergoing Robotic Laparoscopy

Cited by 6 publications

References 12 publications

PURION^® processed human amnion chorion membrane allografts retain material and biological properties supportive of soft tissue repair

PURION^® processed human amnion chorion membrane allografts retain material and biological properties supportive of soft tissue repair

Using of Amniotic Membrane Derivatives for the Treatment of Chronic Wounds

Novel Role of Dehydrated Human Amnion/Chorion Membrane in Healing of Denuded Ureter in Robot-Assisted Excision of Endometriosis

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Evaluation of Dehydrated Human Amnion/Chorion Membrane as an Adhesion Barrier in Women Undergoing Robotic Laparoscopy

Cited by 6 publications

References 12 publications

PURION® processed human amnion chorion membrane allografts retain material and biological properties supportive of soft tissue repair

PURION® processed human amnion chorion membrane allografts retain material and biological properties supportive of soft tissue repair

Using of Amniotic Membrane Derivatives for the Treatment of Chronic Wounds

Novel Role of Dehydrated Human Amnion/Chorion Membrane in Healing of Denuded Ureter in Robot-Assisted Excision of Endometriosis

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PURION^® processed human amnion chorion membrane allografts retain material and biological properties supportive of soft tissue repair

PURION^® processed human amnion chorion membrane allografts retain material and biological properties supportive of soft tissue repair