Evaluation of deep eutectic solvents as co‐solvent for steroids 1‐en‐dehydrogenation biotransformation by <i>Arthrobacter simplex</i>

Mao, Shuhong; Yu, Lu; Ji, Shaoxian; Liu, Xiaoguang; Lu, Fuping

doi:10.1002/jctb.4691

Cited by 52 publications

(47 citation statements)

References 34 publications

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“…As a result, deep eutectic solvents (DESs) currently evolve as a more sustainable alternative granting similar advantages as ILs in comparison to classical organic solvents [39,40]. Compatibility of DESs with purified enzyme preparations has been demonstrated already for enzymes of different classes, but also recently for whole cell catalysts, such as wildtype Arthrobacter [41] or Acetobacter [42] species, baker's yeast [43,44], and recombinant E. coli [45].…”

Section: Ionic Liquids and Deep Eutectic Solventsmentioning

confidence: 99%

Recent advances in whole cell biocatalysis techniques bridging from investigative to industrial scale

Wachtmeister

Rother

2016

Current Opinion in Biotechnology

276

227

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Section: Ionic Liquids and Deep Eutectic Solventsmentioning

confidence: 99%

Recent advances in whole cell biocatalysis techniques bridging from investigative to industrial scale

Wachtmeister

Rother

2016

Current Opinion in Biotechnology

276

227

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Section: Introductionmentioning

confidence: 99%

“…Prednisone acetate (PA) is an important anti‐inflammatory and anti‐allergic glucocorticoid agent . It can be produced by the Δ1, 2‐dehydrogeneration of cortisone acetate (CA) using Arthrobacter simplex , and many attempts have been made to improve the efficiency of this biotransformation . As a promising and attractive alternative to organic solvents and ILs, three ChCl‐based DESs were used as co‐solvents to enhance the efficiency of C1,2‐dehydrogeneration by improving the solubility of the highly hydrophobic substrate .…”

Section: Introductionmentioning

confidence: 99%

Synergistic effects of components in deep eutectic solvents relieve toxicity and improve the performance of steroid biotransformation catalyzed by Arthrobacter simplex

Mao

Hou

et al. 2018

J of Chemical Tech & Biotech

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BACKGROUND The discovery of deep eutectic solvents (DESs) has introduced a new class of promising green solvents to overcome many drawbacks associated with organic solvents. In this study, the synergistic effects of the components in the form of DESs on bacterial viability, membrane integrity, and retention of metabolic activity as well as the efficiency of Δ1,2‐dehydrogeneration of cortisone acetate (CA) using whole cells of Arthrobacter simplex were investigated. RESULTS The agar disc diffusion method, viability assays and the retention of sugar metabolic activity were used to investigate their toxicity to A. simplex. The results indicated that colinium salts were more toxic than the corresponding hydrogen bond donor (HBDs), but their deleterious effect could be weakened by incorporating them into DESs. Moreover, the best catalytic performance was found in the ChCl:U(DES)‐containing system when Δ1,2‐dehydrogeneration using A. simplex was carried out in three different systems followed by the addition of DES, their components and their mixtures, respectively. In addition, metabolites with significantly changed concentration were identified by means of gas chromatography–mass spectrometry (GC–MS) and principal component analysis, which revealed five compounds that may be used as potential indicators to discriminate the effects of different concentrations of DESs on A. simplex. CONCLUSION The cholinium salts used in this study were toxic to A. simplex to some extent, while synergistic effects were observed after DES formation. The efficient biotransformation in DESs especially in the ChCl:U(DES)‐containing system potentially offers a promising strategy for the production of many different steroids. © 2018 Society of Chemical Industry

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“…The use of neoteric solvents, such as ILs andD ESs, could facilitate the necessary step to highs ubstrate loadings. [85] Surprisingly,t he addition of increased amountso ft he DESt ow ater led to complete inversion of the stereoselectivity from S in 95 % ee in pure water to R in 95 % ee at > 80 %D ES.…”

Section: Whole-cell Redox Biocatalysis In Deep Eutectic Solventsmentioning

confidence: 97%

Biocatalysis and Biomass Conversion in Alternative Reaction Media

Sheldon

2016

Chemistry A European J

153

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In this Minireview, the state of the art in the use of ionic liquids (ILs) and deep eutectic solvents (DESs) as alternative reaction media for biocatalytic processes and biomass conversion is presented. Initial, proof-of-concept studies, more than a decade ago, involved first-generation ILs based on dialkylimidazolium cations and non-coordinating anions, such as tetrafluoroborate and hexafluorophosphate. More recently, emphasis has switched to more environmentally acceptable second-generation ILs comprising cations, which are designed to be compatible with enzymes and, in many cases are derived from readily available, renewable resources, such as cholinium salts. Protic ionic liquids (PILs), prepared simply by mixing inexpensive amines and acids, are particularly attractive from both an environmental and economic viewpoint. DESs, prepared by mixing inexpensive salts with, preferably renewable, hydrogen-bond donors such as glycerol and amino acids, have also proved suitable reaction media for biocatalytic conversions. A broad range of enzymes can be used in ILs, PILs and DESs, for example lipases in biodiesel production. These neoteric solvents are of particular interest, however, as reaction media for biocatalytic conversions of substrates that have limited solubility in common organic solvents, such as carbohydrates, nucleosides, steroids and polysaccharides. This has culminated in the recent focus of attention on their use as (co)solvents in the pretreatment and saccharification of lignocellulose as the initial steps in the conversion of second-generation renewable biomass into biofuels and chemicals. They can similarly be used as reaction media in subsequent conversions of hexoses and pentoses into platform chemicals.

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Evaluation of deep eutectic solvents as co‐solvent for steroids 1‐en‐dehydrogenation biotransformation by Arthrobacter simplex

Cited by 52 publications

References 34 publications

Recent advances in whole cell biocatalysis techniques bridging from investigative to industrial scale

Recent advances in whole cell biocatalysis techniques bridging from investigative to industrial scale

Synergistic effects of components in deep eutectic solvents relieve toxicity and improve the performance of steroid biotransformation catalyzed by Arthrobacter simplex

Biocatalysis and Biomass Conversion in Alternative Reaction Media

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