Evaluation of cytotoxicity of two root canal filling materials by MTT assay

Abstract

The aim of this study was to assess the cytotoxicity of two root filling materials GuttaFlow (GF) and gutta-percha (GP) on mouse fibroblasts cell line L-929. In this study there were four groups: GP and GF were considered as study groups and the other two were negative control groups. GP and GF were prepared according to manufacturer's instruction. L-929 fibroblast cells of mouse were passaged with trypsin (Merck, Germany) after elimination of freeze phase. Adequate trypsin was added to cells and they were prepared with 95% of cell vitality. After 24 h, 150,000 cells were put in each well. The cell and dimethyl methacrylate were used as negative and positive controls. Ten specimens from each group were brought into contact with the culture medium and were incubated under sterilised conditions 24 h later. The cytotoxicity of all samples was assessed by dimethylthiazol diphenyltetrazolium bromide test after 1 h, 24 h and 72 h. The results showed that cytotoxicity of GF was less than GP when assessed at 24 h and 72 h, but there was no significant difference at 1 h. In GF, the most and least cytotoxicity were observed at 24 h and 72 h while cytotoxicity of GP increased with time.

Biomechanical properties of nano-TiO2 addition to a medical silicone elastomer: The effect of artificial ageing

“…17 Since the 2% concentrations of nanoTiO 2 increase the elongation at break of the silicone elastomer, this may improve the deformation characteristics of facial epitheses. This may be due to the high specific surface area of nano-TiO 2 , which is likely to reinforce the contact area and the 18 Studies show that the interaction between the fine particulate filler and matrix can increase the elongation at break of the filling system. 19 However, the concentration of nanoparticles in silicone elastomers at 4% and 6% exhibited a certain degree of agglomeration, in which the molecular chains were fixed more firmly around the nanoparticles, weakening the interaction with the silicone elastomer.…”

Tissue reaction to sealing materials: different view at biocompatibility

“…When compared to AHPlus, the gutta percha based sealer is significantly less cytotoxic, when assessing the viability and the proliferation of cells by means of the MTT-test (3-(4,5-Dimethylthiazol-2-yl)-2,5diphenyltetrazoliumbromid) [14]. The observed low cytotoxicity appears to be independent of the cell origin since very low cytoxicity was reported after incubation of mouse fibroblasts on GuttaFlow [15]. In a further in vitro study GuttaFlow was tested on human peripheral blood lymphocytes using the comet assay and chromosomal aberration analysis.…”

The sealing ability of a new silicone-based root canal filling material (GuttaFlow): an in vitro study using the percentage of gutta-percha-filled area

“…It is also widely mentioned in endodontic literature that the cytotoxicity of GuttaFlow is less than GP or other root canal sealers [40][41][42] , and that it has acceptable biocompatibility in terms of genotoxicity 43) . Therefore, whether the extruded GuttaFlow might pose a potential damage to the periapical tissue would require further studies to clarify and confirm.…”