Tissue reaction to sealing materials: different view at biocompatibility

Ghanaati, Shahram; Willershausen, Inés; Barbeck, Mike; Unger, Ron; Joergens, M; Sader, RA; Kirkpatrick, Charles James; Willershausen, Brita

doi:10.1186/2047-783x-15-11-483

Cited by 23 publications

(31 citation statements)

References 24 publications

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“…Furthermore, all the groups demonstrated intense granulation reaction with the presence of a larger number of blood vessels, which clinically suggested the presence of hyperemic areas (P=0.002). The granulation reaction induced by the cements was predictable in the initial period, since it functioned as a pathway for the macrophages to reach the implant site (Ghanaati et al 2010).…”

Section: Discussionmentioning

confidence: 99%

“…After the period of 7 days, the RMO ® and Transbond ® demonstrated intense inflammatory infiltrate when compared with the control group (P=0.004). This initial inflammatory response reflected the attempt by the tissue to induce the process of degradation of the materials through the inflammatory cells recruited to the surrounding tissue and in the direction towards the surface of the cements (Ghanaati et al 2010, Malkoc et al 2010. In addition, it suggested that the elution of toxic substances released by these cements was capable of stimulating the production of reactive oxygen species (ROS), and increasing the expression of COX-2/PGE 2, which are potent chemical mediators, capable of potentiating the inflammatory response (Costa et al 2011).…”

Section: Discussionmentioning

confidence: 99%

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Evaluation in vivo of biocompatibility of differents resin-modified cements for bonding orthodontic bands

Mesquita

Santos

Sampaio

et al. 2017

An. Acad. Bras. Ciênc.

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The focus of this study was to test the hypothesis that there would be no difference between the biocompatibility of resin-modified glass ionomer cements. Sixty male Wistar rats were selected and divided into four groups: Control Group; Crosslink Group; RMO Group and Transbond Group. The materials were inserted into rat subcutaneous tissue. After time intervals of 7, 15 and 30 days morphological analyses were performed. The histological parameters assessed were: inflammatory infiltrate intensity; reaction of multinucleated giant cells; edema; necrosis; granulation reaction; young fibroblasts and collagenization. The results obtained were statistically analyzed by the Kruskal-Wallis and Dunn test (P<0.05). After 7 days, Groups RMO and Transbond showed intense inflammatory infiltrate (P=0.004), only Group RMO presented greater expression of multinucleated giant cell reaction (P=0.003) compared with the control group. After the time intervals of 15 and 30 days, there was evidence of light/moderate inflammatory infiltrate, lower level of multinucleated giant cell reaction and thicker areas of young fibroblasts in all the groups. The hypothesis was rejected. The Crosslink cement provided good tissue response, since it demonstrated a lower level of inflammatory infiltrate and higher degree of collagenization, while RMO demonstrated the lowest level of biocompatibility.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Evaluation in vivo of biocompatibility of differents resin-modified cements for bonding orthodontic bands

Mesquita

Santos

Sampaio

et al. 2017

An. Acad. Bras. Ciênc.

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“…The aim of root canal filling is to promote the hermetic sealing of root canals, neutralize the remaining microbiota and stimulate mineralization of the pulp stump without causing toxic effects on the cells of the periapical region (1). Therefore, the biological properties of root canal sealers and the degree of cytotoxicity or stimulation they may have on different cells, including fibroblasts, osteoblasts and cementoblasts must be evaluated (2,3).…”

Section: Introductionmentioning

confidence: 99%

“…Studies have evaluated the biological effects of sealers based on zinc-oxide-eugenol (ZOE), glass ionomer, epoxy resin, silicones, calcium silicate, methacrylate resin and sealers containing calcium hydroxide in their composition (1). The in vitro cytotoxicity of different types of sealers has been evaluated by direct exposure of different cell lines to sealers or their components solubilized in liquid media (4)(5)(6).…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity Evaluation of Root Canal Sealers Using an In Vitro Experimental Model with Roots

et al. 2017

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The aim of the present study was to evaluate the cytotoxicity of root canal sealers under conditions closely resembling a clinical reality. A primary human fibroblast cell line was seeded in 24-well acrylic plates with Dulbecco's modified Eagle's medium supplemented with 10% serum fetal bovine (SFB) and incubated for 24 h. Root canals from premolars were filled and individually attached to nylon devices to be stabilized in the wells with the already seeded cells. Specimens were divided into groups as follows: Control: gutta-percha cones (GPC); AH Plus+GPC; Sealapex+GPC; MTA Fillapex+GPC and Endofill+GPC. After 24 and 48 h, cell viability and morphology were evaluated by MTT assay and scanning electron microscopy (SEM), respectively. Statistical analysis was performed by Mann-Whitney test, complemented by Kruskal Wallis test (p<0.05). Only Endofill presented cytotoxicity after 24 h. MTA Fillapex and Endofill reduced the production of succinic desidrogenase after 48 h. AH Plus was non-toxic at any time point. SEM showed that the AH Plus and MTA Fillapex groups presented fibroblasts with morphology close to the control group, while the Endofill group presented few cells with thin extensions cells. The present study showed that good results were present in AH Plus and Sealapex, but not the Endofill group after 48 h. The method used enabled evaluation of the cytotoxicity of the studied sealers that diffused through the root apex.

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“…Healing after treatment depends directly on the characteristics of the material used for canal sealing. According to the literature, the materials should not cause irritation of the periapical tissue, because this may result in failure of endodontic treatment (1).…”

Section: Introductionmentioning

confidence: 99%

Bone Tissue Response to Endomethasone Implanted into the Rat Mandible / Reakcija koštanog tkiva na endometazon implantiran u mandibulu pacova

Nikolić¹,

Gasic²,

Mitić³

et al. 2015

Acta Facultatis Medicae Naissensis

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The aim of the paper was to investigate the tissue response to the implantation of "Endomethasone" (Septodont) in artificially prepared defect in the rat mandible.Sixteen Wistar male rats were used for the experiment. After anesthesia, a defect between the midline and the mental foramen of the left mandible was made (diameter 1.4 mm, 1.6 mm depth) using sterile steel burs. In the defect of the experimental group (12 animals) "Endomethasone" was implanted while the defect in the control group (4 animals) was left to heal spontaneously. One half of animals of both groups was sacrificed after fifteen days, and the second half after sixty days. The samples consisted of the defect and the surrounding bone. After routine decalcination and processing, the samples were embedded in paraffin, and microscopic preparations were made, on the basis of which a microscopic analysis was performed.Fifteen-day-old specimens showed the early signs of bone resorption, as well as granulated connective tissue, hyperemic blood vessels, fibrin exudate to a lesser extent, with the difference that inflammatory infiltrates were less present in the experimental group. Sixty-day-old specimens demonstrated the degree of bone healing; tissue filling with newly formed bone was significantly more advanced in the control group. Experimental group showed a sign of delay with significant presence of callus tissue that more in the form of focus showed a deposition of the young bone tissue.Endomethasone masks the inflammatory reaction which follows operational procedures. In the long run, according to the results of the present research, Endomethasone can interfere with bone healing by causing delayed inflammatory reaction.

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Tissue reaction to sealing materials: different view at biocompatibility

Cited by 23 publications

References 24 publications

Evaluation in vivo of biocompatibility of differents resin-modified cements for bonding orthodontic bands

Evaluation in vivo of biocompatibility of differents resin-modified cements for bonding orthodontic bands

Cytotoxicity Evaluation of Root Canal Sealers Using an In Vitro Experimental Model with Roots

Bone Tissue Response to Endomethasone Implanted into the Rat Mandible / Reakcija koštanog tkiva na endometazon implantiran u mandibulu pacova

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