Evaluation of cytotoxic properties of Curcuma longa and Tagetes erecta on cancer cell line (Hep2)

Abstract

Cancer is the major public difficulty and one of the top causes of death in prosperous countries. Conventional plants are precious source of novel cytotoxic agents and play a critical role in health concern. Due to its wide range of biological and pharmacological effects and lack of toxicity, curcumin and lutein were selected for this study. Curcumin and lutein were isolated from rhizomes of Curcuma longa and petals of Tagetes erecta. The isolated pigments were quantified spectroscopically and separated by thin layer chromatography. The active components of the pigments were further purified and identified by high performance liquid chromatography. In vitro cytotoxic activity of both extracts against Hep2 cancer cell lines were evaluated. Furthermore, the activities of both pigments in different concentrations against Hep2 cancer cell line were compared. The test sample showing cell viability of more than 97% at 0.078 mg/ml were considered to be less active at minimum concentration. The maximum viability of Hep2 cell line were 3.27% (curcumin) and 8.88% (lutein), respectively, which are most suitable to perform cytotoxic studies. This method suggests that it is suitable for the rapid screening of plant materials and also can be performed without any special sample pretreatment.

Screening of Chemical Characterization, Antifungal and Cytotoxic Activities of Essential Oil Constituents of <i>Tagetes erecta</i> L. from Erbil, Kurdistan Region-Iraq

“…A much lower amount of viable cells (less than 10%) were detected at this range of concentrations, which showed the maximum inhibition concentration. These results were in accordance with previous studies performed on T. erecta conducted by Gupta and Gupta [6], Vallisuta and Nukoolkarn [25], and Ayyadurai and Valarmathy [26]. Interestingly, the study conducted by [7] for determining the cytotoxic activity of the essential oils of four plants (T. erecta L., Tetradenia riparia, Bidens sulphurea, and Foeniculum vulgare) against six tumor cell lines murine melanoma (B16F10), human colon carcinoma (HT29), human breast adenocarcinoma (MCF-7), human cervical adenocarcinoma (HeLa), human hepatocellular liver carcinoma (HepG2), and human glioblastoma (MO59J, U343, and U251), showed that cancer cells have higher sensitivity to the oil of T. erecta.…”

Effective Medicinal Plant in Cancer Treatment, Part 2: Review Study

“…The study of cytotoxic properties of turmeric on liver cancer cells (Hep-2) showed that the cytotoxicity mediated by curcumin in a dose-dependent manner leads to apoptosis of cancer cells through mitochondrial pathway. 83 …”

Diversity of medicinal plants utilized by To Manui ethnic of Central Sulawesi, Indonesia

“…C. longa is used by traditional healers in To Manui ethnic to treat various symptoms/diseases such as diabetes, pre, and post-natal care and flu/colds. Curcumin contained in C. longa and most species of the Zingiberaceae family are the main components responsible for various pharmacological effects including antidiabetic, anticancer, antiinflammatory, antimicrobial, antifungal, anti-HIV and as hepato-and nephroprotector (Kurapati and Samikkannu 2012;Ayyadurai et al 2013;Rafieian-Kopaei et al 2014;Hamidpour et al n.d.;Schmidt et al 2015;Shukla et al 2016). Olatunde et al (2014) reported oral administration of water extract C. longa 200 mg/kg bw for 28 days had an antihyperglycemic effect in diabetic rats induced by alloxan.…”