Evaluation of cytotoxic potential of chlorpyrifos using Lens culinaris Med as efficient bioindicator

Mercado, Seir Antonio Salazar; Bayona, Hanner Alejandra Maldonado

doi:10.1016/j.ecoenv.2019.109528

Cited by 15 publications

(9 citation statements)

References 51 publications

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“…The different results obtained after determining the cytotoxicity of diazinon suggested that cell viability is dependent on the pesticide tested [ 90 ]. Chlorpyrifos exposure has a strong cytotoxic effect on Lens culinaris apical cells and induces chromosomal abnormalities such as chromosome disruption, nucleus absence, irregular anaphase, micronuclei, and anaphase bridges [ 91 ]. Some chemical compounds exhibiting antioxidant properties can decrease the cytotoxicity of chlorpyrifos and reduce the inhibition of catalase and glutathione peroxidase activity caused by this insecticide [ 92 ].…”

Section: Resultsmentioning

confidence: 99%

Characterization and Protective Properties of Lactic Acid Bacteria Intended to Be Used in Probiotic Preparation for Honeybees (Apis mellifera L.)—An In Vitro Study

Leska

Nowak

Rosicka‐Kaczmarek

et al. 2023

Animals

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Lactic acid bacteria (LAB) are widely used probiotics and offer promising prospects for increasing the viability of honeybees. Thus, the probiotic potential of 10 LAB strains was determined, which in our previous studies showed the most potent protective abilities. In the current study, we investigated various properties of probiotic candidates. The tested LAB strains varied in susceptibility to tested antibiotics. Isolates showed high viability in sugar syrups and gastrointestinal conditions. None of the LAB strains exhibited β-hemolytic activity, mutual antagonism, mucin degradation, hydrogen peroxide production capacity, or bile salt hydrolase (BSH) activity. Additionally, the cytotoxicity of LAB cell-free supernatants (CFS) was assessed, as well as the effect of CFS from P. pentosaceus 14/1 on the cytotoxicity of coumaphos and chlorpyrifos in the Caco-2 cell line. The viability of Caco-2 cells reached up to 89.81% in the presence of the highest concentration of CFS. Furthermore, LAB metabolites decreased the cytotoxicity of insecticides (up to 19.32%) thus demonstrating cytoprotective activity. All tested LAB strains produced lactic, acetic, and malonic acids. This research allowed the selection of the most effective LAB strains, in terms of probiosis, for future in vivo studies aimed at developing an ecologically protective biopreparation for honeybees.

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Section: Resultsmentioning

confidence: 99%

Characterization and Protective Properties of Lactic Acid Bacteria Intended to Be Used in Probiotic Preparation for Honeybees (Apis mellifera L.)—An In Vitro Study

Leska

Nowak

Rosicka‐Kaczmarek

et al. 2023

Animals

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“…Therefore, inhibition of root growth can be used to demonstrate the cytotoxicity of substances [ 45 , 66 , 67 ]. Also, growth inhibition can be caused by a reduction in mitotic activity as well as the appearance of several chromosomal aberrations [ 45 , 66 ].…”

Section: Discussionmentioning

confidence: 99%

“…The most frequently used plants for toxicity testing are Allium cepa L., Lens culinaris Med [ 42 , 45 , 46 ], Pisum sativum L [ 47 ], Cladonia verticillaris Raddi, Lactuca sativa L. [ 48 ] and Vicia faba L. [ 49 ]. In general, plants belonging to the genus Allium, especially Allium cepa , are considered the most useful and effective for genotoxicity assessment due to their advantages [ 42 , 50 ].…”

Section: Introductionmentioning

confidence: 99%

“…The Allium test can determine several cytogenic and genotoxic parameters, such as mitotic index, phase indices, chromosomal aberrations, mitotic abnormalities, and micronuclei formation [ 44 , 50 , 54 , 55 , 56 ]. Chromosomal aberrations and micronuclei formation in meristematic cells of Allium cepa have been used as reliable biological indicators and predictive markers for xenobiotic-induced DNA damage [ 45 , 46 , 54 , 56 , 57 ]. The Allium test has been reported to be sensitive and can detect the genotoxic and mutagenic potential of any natural, chemical, or synthetic compound.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Indigo Carmine on Growth, Cell Division, and Morphology of Allium cepa L. Root Tip

Ristea,

Zarnescu

2024

Toxics

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Indigo carmine has a variety of uses in foods, textiles, medicine, pharmaceuticals, and cosmetics. There are studies reporting the toxic potential of indigo carmine on human health and the environment. In this study, we investigated the cytogenotoxic effects of indigo carmine using apical root cells of Allium cepa. Allium cepa bulbs were subjected to four treatments with indigo carmine (0.0032, 0.0064, 0.0125, and 0.2 mg/mL) and to ultrapure water as a control. After 5 days, root growth, root length, mitotic index, mitotic inhibition, chromosomal anomalies, and cell morphology were analyzed. According to our results, a decrease in root length and mitotic index was observed at all concentrations of indigo carmine. Additionally, several types of chromosomal abnormalities were observed, such as disturbed metaphase, sticky chain metaphase, anaphase bridge, and laggard chromosomes. Moreover, histological observation indicated that indigo carmine induces alterations in various components of root tip tissue, such as deformation and alteration of the cell wall, progressive condensation of chromatin, shrinkage of the nuclei, and an increase in the number of irregularly shaped nuclei and nuclear fragments. Our results indicate that the tested concentrations of indigo carmine may have toxic effects and raise concerns about its intensive use in many fields.

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“…La germinación de las semillas de C. annuum puede variar y a menudo es lenta y no uniforme en condiciones de estrés abióticos, tales como alta salinidad, toxicidad por agroquímicos o bajas temperaturas [14,15,16,17,12]. De igual manera, aspectos genéticos, condición del suelo y el estado físico de las semillas influencian la geminación [18].…”

Section: Introductionunclassified

Determinación de la viabilidad de semillas de capsicum annuum L usando la prueba de tetrazolio

Mercado

Quintero-Caleño

Rojas-Suárez

2020

AiBi Revista de Investigación, Administración e Ingeniería

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El pimentón (Capsicum annuum L) es uno de los cultivos de hortalizas más importantes y ampliamente cultivados a nivel mundial. Este estudio determinó la eficacia del test tetrazolio para obtener la viabilidad de semillas de C. annuum. De manera que, semillas de C. annuum se expusieron a dos concentraciones (1% y 0.5%) de tetrazolio y dos tiempos de exposición (24 y 48horas). Ulteriormente, utilizando un microscopio estereoscopio se valoró la tinción de las semillas, estimando como semillas viables las que presentaron coloración roja en el embrión. Por consiguiente, se obtuvieron diferencias estadísticamente significativas (P ≤ 0,05: Tukey HSD) en el tratamiento T4 (0.5 %, 48h) con un 99% de viabilidad. Así mismo, los tratamientos T1 (1 %, 24h) y T2 (0.5 %, 24h) no presentaron diferencias entre sí, siendo el porcentaje de viabilidad 89% y 91% respectivamente. Para la prueba de germinación real, se obtuvo un 84.4% (promedio) de germinación para las 5 repeticiones. Se concluye que mediante el ensayo de tetrazolio la evaluación de la viabilidad en semillas de pimentón es eficiente para las concentraciones de 1% y 0.5 %, durante 24 horas.

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Evaluation of cytotoxic potential of chlorpyrifos using Lens culinaris Med as efficient bioindicator

Cited by 15 publications

References 51 publications

Characterization and Protective Properties of Lactic Acid Bacteria Intended to Be Used in Probiotic Preparation for Honeybees (Apis mellifera L.)—An In Vitro Study

Characterization and Protective Properties of Lactic Acid Bacteria Intended to Be Used in Probiotic Preparation for Honeybees (Apis mellifera L.)—An In Vitro Study

Effects of Indigo Carmine on Growth, Cell Division, and Morphology of Allium cepa L. Root Tip

Determinación de la viabilidad de semillas de capsicum annuum L usando la prueba de tetrazolio

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