Evaluation of Cytotoxic effect of Garlic on human gingival fibroblasts: A Preliminary Study

Abstract

In this study we aimed to evaluate the cytotoxic effect of garlic extract on human gingival fibroblasts. Garlic extracts were prepared at 5 different concentrations as 12.5%, 6.25%, 3.12%, 1.5%, and 0.6%. Cytotoxic effects of garlic and clorhexidine gluconat (CH) on human gingival fibroblasts were evaluated by agar diffusion method at 24 h. Garlic extracts concentrations at 6.25, 3.12, and 1.5% were not found cytotoxic on human gingival fibroblasts, whereas concentration at 12.5% was found cytotoxic, this effect was found less cytotoxic than CH. According to results of this study we could suggest that Garlic would have a promising role in the future for oral health, if appropriate solutions can be prepared.

Isolation of Actinobacillus Actinomycetumcomitans (Aggregobacter) Andporphyromonas Gingivalis From Sudanese Aggressive Periodontitis Patients and Testing Inhibitory Effect of Garlic Extract Compared to Chlorhexidine Digluconate 0.2%.

“…( (29,30,31,32) .Garlic extracts in concentrations of 12.5% is cytotoxic to human gingival fibroblasts but it is safe at 6.25, 3.12, and 1.5% , the high concentration of garlic is less cytotoxic than chlorhexidinedigluconate. (33) In Sudan there are many studies on the prevalence of aggressive periodontitis among different age groups, the A. actinomycetemcomitan were detected in the subgingival plaque of 12 (70.6%) patients with aggressive periodontitis and from only one (5.9%) control subject, showing a significantly higher frequency of detection in cases than in controls. The JP2 clone of A. actinomycetemcomitans was not detected in the subgingival plaque of high school subjects in Sudan.…”

Amoebicidal effect of Allium cepa against Allovahlkampfia spelaea: A keratitis model

“…The plates were incubated for 24 h at 37 °C in a 5% CO 2 incubator, and then the cell lysis was observed by an inverted microscope and scored. One score was given for each sample, and the median value for the parallel samples was calculated for the lysis zone ( Özan et al, 2013 , Polat et al, 2007 ).…”

Ethanolic Garlic Extract (Allium sativumL) Increased Viability and Proliferation of Human Gingival Fibroblast In Vitro

“…This was accordance with previous study that garlic extract with concentration 50% to 100% did not have cytotoxic effect to fibroblast cell culture BHK-21. 18 However, Ozan et al (2013) suggested that garlic extract had mild cytotoxic effect to fibroblasts in vitro, but when compared with chlorhexidine as a positive control, chlorhexidine still more toxic. 17 The garlic extract has no toxic effects on the proliferation of fibroblasts both the lowest and highest concentration.…”

“…18 However, Ozan et al (2013) suggested that garlic extract had mild cytotoxic effect to fibroblasts in vitro, but when compared with chlorhexidine as a positive control, chlorhexidine still more toxic. 17 The garlic extract has no toxic effects on the proliferation of fibroblasts both the lowest and highest concentration. The results of the regression analysis on the entire time observations have shown no correlation between the magnitude of the high concentration of cell viability, even though dilution with the lowest concentration of 0.3125 microg / ml still showed a high viability (above 100%).…”

“…However garlic is a herbal, we have to make sure that's garlic must not have a detrimental effect on the biological environment, both local and systemic. 17 Therefore, our study aimed to analyze the biocompatibility of garlic extract by observing the viability and proliferation of human gingival fibroblasts in vitro.…”