Amoebicidal effect of Allium cepa against Allovahlkampfia spelaea: A keratitis model

Eldeek, Hanan E. M.; Farrag, Haiam Mohamed Mahmoud; Tolba, Mohammed Essa Marghany; El-Deek, Heba E M; Ali, Marwa Omar; Ibraheim, Zedan Z.; Bayoumi, Soad A. L .; Hassanin, Ebtisam Shawky Ahmed; Alkhalil, Samia S.; Huseein, Enas Abd El Hameed Mahmoud

doi:10.1016/j.jsps.2022.06.005

Cited by 1 publication

(1 citation statement)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For untreated control, 100 μL of PBS was added to 100 μL of A. castellanii suspension. Each concentration was tested in triplicates and incubated at 26 °C at different periods (3,6,9,12, and 24 h) 25 . The viability of A. castellanii trophozoite/cyst was assessed using 0.4% trypan blue, which selectively stains non-viable cells.…”

Section: Amoebicidal Activity Of the Extract Against Trophozoites And...mentioning

confidence: 99%

Combating the causative agent of amoebic keratitis, Acanthamoeba castellanii, using Padina pavonica alcoholic extract: toxicokinetic and molecular docking approaches

Abdel-Hakeem,

Hassan,

Hifney

et al. 2024

Sci Rep

View full text Add to dashboard Cite

Natural products play a significant role in providing the current demand as antiparasitic agents, which offer an attractive approach for the discovery of novel drugs. The present study aimed to evaluate in vitro the potential impact of seaweed Padina pavonica (P. pavonica) extract in combating Acanthamoeba castellanii (A. castellanii). The phytochemical constituents of the extract were characterized by Gas chromatography–mass spectrometry. Six concentrations of the algal extract were used to evaluate its antiprotozoal activity at various incubation periods. Our results showed that the extract has significant inhibition against trophozoites and cysts viability, with complete inhibition at the high concentrations. The IC50 of P. pavonica extract was 4.56 and 4.89 µg/mL for trophozoites and cysts, respectively, at 24 h. Morphological alterations of A. castellanii trophozoites/cysts treated with the extract were assessed using inverted and scanning electron microscopes and showed severe damage features upon treatment with the extract at different concentrations. Molecular Docking of extracted compounds against Acanthamoeba cytochrome P450 monooxygenase (AcCYP51) was performed using Autodock vina1.5.6. A pharmacokinetic study using SwissADME was also conducted to investigate the potentiality of the identified bioactive compounds from Padina extract to be orally active drug candidates. In conclusion, this study highlights the in vitro amoebicidal activity of P. pavonica extract against A. castellanii adults and cysts and suggests potential AcCYP51 inhibition.

show abstract

Section: Amoebicidal Activity Of the Extract Against Trophozoites And...mentioning

confidence: 99%

Combating the causative agent of amoebic keratitis, Acanthamoeba castellanii, using Padina pavonica alcoholic extract: toxicokinetic and molecular docking approaches

Abdel-Hakeem,

Hassan,

Hifney

et al. 2024

Sci Rep

View full text Add to dashboard Cite

show abstract

Amoebicidal effect of Allium cepa against Allovahlkampfia spelaea: A keratitis model

Cited by 1 publication

References 53 publications

Combating the causative agent of amoebic keratitis, Acanthamoeba castellanii, using Padina pavonica alcoholic extract: toxicokinetic and molecular docking approaches

Combating the causative agent of amoebic keratitis, Acanthamoeba castellanii, using Padina pavonica alcoholic extract: toxicokinetic and molecular docking approaches

Contact Info

Product

Resources

About