Evaluation of Cytotoxic Effect, Anti-Cholinesterase, Hemolytic and Antibacterial activities of the Species Scabiosa stellata L.

Abstract

Objective: In this study, cytotoxic effect, anticholinesterase, hemolytic and antibacterial activities of crude extracts (petroleum ether, ethyl acetate and n-butanol) obtained from the plant Scabiosa stellata L. were evaluated. Methods: The cytotoxicity of extracts was tested by Brine shrimp lethality method; the acetylcholinesterase inhibitory activity was performed using Ellman's colorimetric method and the hemolytic activity was assessed by spectrophotometric method towards human erythrocytes. Furthermore, the antibacterial activity was estimated by agar disk diffusion assay against ten bacterial strains. Results: The phytochemical screening of the extracts revealed the presence of several types of secondary metabolites. A significant cytotoxic effect was observed for the n-butanolic extract with 57.2 ± 0.2 % of mortality at 80 μg/mL, the ethyl acetate extract had a moderate anticholinesterase activity at 200 μg/mL. The hemolytic assay exhibited that n-butanolic and ethyl acetate extracts induce hemolysis in dose-dependent manner with values of EC50 at 37.3 ± 0.5 and 106.6 ± 0.3 μg/mL, respectively. All the crude extracts showed antibacterial activity against most tested strains, with zones of inhibition ranging from 9 to 20 mm. Conclusion: The results indicate that the extracts obtained from S. stellata can be an important source of therapeutic agents against pathological damage due to free radicals inducing neurodegenerative and infectious diseases, while n-butanolic extract could be used as a good source of alternative natural antiproliferative compounds.

Combined experimental, computational studies (synthesis, crystal structural, DFT calculations, spectral analysis) and biological evaluation of the new homonuclear complex Di-µ-benzoato-bis [benzoatodipyridine-cobalt (II)]

“…The antibacterial activity of the complex "di-µ-benzoato-bis [benzoatodipyridine-cobalt (II)" with general formula Co 2 (      Benz) 2 (  Benz) 2 (Pyr) 4 was evaluated by the agar disk diffusion assay [19], against six bacterial strains. One or several colonies from each pure culture were transferred into 5 mL of nutrient broth.…”

“…Their atomic charges increase with the oxidation state of the system C(1) from 0.166 to 0.200 e -, and C(8) from 0.172eto 0.207 e -. All other carbon atomic charges C(20), C(19), C(15), and C(24) remain unchanged during oxidation.…”

Dipsacus and Scabiosa Species—The Source of Specialized Metabolites with High Biological Relevance: A Review

“…With respect to Scabiosa genera, many studies indicate that species of this genus show antioxidant [42,43,46,50,52,53,56,[72][73][74][75][76][77], anti-inflammatory [50], anti-diabetic [43,78], antihepatic fibrosis [47,79,80], anti-cancer [56,81], and antibacterial [56,75,77,82,83] properties. Many species of Scabiosa grow naturally in the Mediterranean region [55,56].…”

SARS-CoV-2: Pathogenesis, Molecular Targets and Experimental Models

“…Chemical modification of phytochemicals has been reported to increase the potency and selectivity against SARS-CoV-2 infection. Experimental studies show that natural compounds like quercetin, caffeic acid, and myricetin act as inhibitors of SARS-CoV-2 infection (Mani et al, 2020;Mouffouk et al, 2021).…”