Evaluation of cytogenetic effects of lambda-cyhalothrin on Wistar rat bone marrow by gavage administration

Çelık, Ayla; Mazmanci, Birgül; Çamlica, Yusuf; Çömelekoğlu, Ülkü; Aşkın, A.

doi:10.1016/j.ecoenv.2004.07.009

Cited by 55 publications

(24 citation statements)

References 29 publications

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“…Previous studies have affi rmed the genotoxic and/or cytotoxic effects of Type II pyrethroids such as cyfl uthrin, cypermethrin etc. (4)(5)(6)(7)(8)(9). The carcinogenic potential of pyrethroids was also reported on the murine model (9).…”

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confidence: 87%

Altered canonical hedgehog-gli signalling axis in pesticide-induced bone marrow aplasia mouse model

Chaklader¹,

Das²,

Pereira³

et al. 2012

Archives of Industrial Hygiene and Toxicology

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The mechanistic interplay between pesticide exposure and development of marrow aplasia is not yet well established but there are indices that chronic pesticide exposure in some instances causes marrow aplasia like haematopoietic degenerative condition in human beings. Canonical Hedgehog (Hh) signalling has multiple roles in a wide range of developmental processes, including haematopoiesis. The present study was designed to explore the status of four important components of the canonical Hedgehog signalling cascade, the Sonic Hedgehog (Shh), Ptch1, Smo, and Gli1, in a mouse model of chronic pesticide-induced bone marrow aplasia. We used 5 % aqueous mixture of pesticides (chlorpyriphos, prophenophos, cypermethrin, alpha-methrin, and hexaconazole) for inhalation and dermal exposure of 6 hours per day and 5 days a week up to 90 days. Murine bone marrow aplasia related to chronic pesticide treatment was confi rmed primarily by haemogram, bone marrow cellularity, short term bone marrow explant culture for cellular kinetics, bone marrow smear, and fl ow cytometric Lin -Sca-1 + C-kit + extracellular receptor expression pattern. Later, components of hedgehog signalling were analysed in the bone marrow of both control and pesticide-treated aplastic groups of animals. The results depicted pancytopenic feature of peripheral blood, developmental anomaly of neutrophils, depression of primitive stem and progenitor population along with Shh, Ptch1, Smo and Gli1 expression in aplasia group. This investigation suggests that pesticide-induced downregulation of two critically important proteins -Ptch1 and Gli1 -inside the haematopoietic stem and progenitor cell population impairs haematopoietic homeostasis and regeneration mechanism in vivo concurrent with bone marrow aplasia. Arh Hig Rada Toksikol 2012;63:271-282 A surge of numerous organophosphates, pyrethroids (analogs of naturally occurring phytopyrethrins), and thiazole fungicides replaced DDT after the ban in 1993. Depending on the physiological activity, synthetic pyrethroids are of two distinct types: Type I pyrethroids, which do not contain the α-cyano group, and Type II pyrethroids, which have the α-cyano group (1-3). Previous studies have affi rmed the genotoxic and/or cytotoxic effects of Type II pyrethroids such as cyfl uthrin, cypermethrin etc. (4-9). The carcinogenic potential of pyrethroids was also reported on the murine model (9). A few recent studies revealed that besides blocking neurotransmission, both pyrethroids and organophosphate pesticides can cause Unauthenticated Download Date | 5/12/18 12:23 AM KEY WORDS: bone marrow suppression, haematopoietic stem cell, patched, smoothened, sonic hedgehog Chaklader M, et al. ALTERED HH-GLI SIGNALLING IN PESTICIDE-INDUCED BONE MARROW APLASIA

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confidence: 87%

Altered canonical hedgehog-gli signalling axis in pesticide-induced bone marrow aplasia mouse model

Chaklader¹,

Das²,

Pereira³

et al. 2012

Archives of Industrial Hygiene and Toxicology

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“…The genotoxic and/or cytotoxic potential of some Type II pyrethroid insecticides such as cyfluthrin [Tisch et al, 2005], cypermethrin [Amer and Aboul-Ela, 1985;Bhunya and Pati, 1988;Amer et al, 1993;Chauhan et al, 1997Chauhan et al, , 1999Hadnagy et al, 1999;Institóris et al, 1999;Giri et al, 2003;Patel et al, 2006;Suman et al, 2006], deltamethrin [Agarwal et al, 1994;Surrallés et al, 1995;Gandhi et al, 1995;Villarini et al, 1998], fenvalerate [Pati and Bhunya, 1989;Puig et al, 1989;Surrallés et al, 1990;Caballo et al, 1992;Ghosh et al, 1992;Giri et al, 2002], and k-cyhalothrin [Campana et al, 1999;Ç elik et al, 2003, 2005a, 2005bNaravaneni and Jamil, 2005] has been shown in previous studies.…”

Section: Introductionmentioning

confidence: 99%

The in vitro genotoxic effects of a commercial formulation of α‐cypermethrin in human peripheral blood lymphocytes

Kocaman

Topaktaş

2008

Environ and Mol Mutagen

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a-Cypermethrin, a highly active pyrethroid insecticide, is effective against a wide range of insects encountered in agriculture and animal husbandry. The potential genotoxicity of a commercial formulation of a-cypermethrin (Fastac 100 EC, containing 10% a-cypermethrin as the active ingredient) on human peripheral lymphocytes was examined in vitro by sister chromatid exchange (SCE), chromosomal aberrations (CAs), and micronucleus (MN) tests. The human lymphocytes were treated with 5, 10, 15, and 20 lg/ml of a-cypermethrin for 24-and 48-hr. a-Cypermethrin induced SCEs and CAs significantly at all concentrations and treatment times and MN formation was significantly induced at 5 and 10 lg/ml of a-cypermethrin when compared with both the control and solvent control. Binuclear cells could not be detected sufficiently in the highest two concentration of a-cypermethrin (15 and 20 lg/ml) for both the 24-and 48-hr treatment times. a-Cypermethrin decreased the proliferation index (PI) at three high concentrations (10, 15, and 20 lg/ml) for both treatment periods as compared with the control groups. In addition, acypermethrin reduced both the mitotic index (MI) and nuclear division index (NDI) significantly at all concentrations for two treatment periods. The PI and MI were reduced by a-cypermethrin in a concentration-dependent manner during both treatment times. In general, a-cypermethrin showed higher cytotoxic and cytostatic effects than positive control (MMC) at the two highest concentrations for the 24-and 48-hr treatment periods. The present study is the first to report the genotoxic and cytotoxic effects of commercial formulation of a-cypermethrin in peripheral blood lymphocytes. Environ. Mol. Mutagen. 50:27-36, 2009. V V C 2008 Wiley-Liss, Inc.

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“…This increase may denote an increase in metabolic transport [63] which may eventually result in a shift in biosynthesis and the energy metabolism pathway of the exposed organism [64]. The decrease in ACP activity in the tissues reflects a possible decrease in biosynthetic activities and anaerobic capacity of C. gariepinus [65], while an elevation in ACP, suggests an increase in lysosomal mobilization and cell necrosis due to effluent toxicity [66].…”

Section: Discussionmentioning

confidence: 99%

Enzymatic responses of Clarias gariepinus (Burchell, 1822) exposed to sub-lethal concentrations of an oilfield wastewater

Akani¹,

Gabriel²

2016

J App Biol Biotech

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Twenty eight adult Clarias gariepinus (mean weight 205 ± 12.89g SD; Mean length; 31.13 ± 3.82cm SD) were exposed to oilfield wastewater, oww (0. 10, 20, 30, 40, 50 and 60%) in quadruplicate for 28 days and the organs (plasma, gill, liver, kidney) and muscle were assayed for enzymes: aspartate transaminase (AST), alanine transaminase (ALT), acid phosphatase (ACP) alkaline phosphates (ALP) and lactate dehydrogenase (LDH). Changes in enzyme response were not concentration-dependent (P < 0.05), except ALP in kidney which showed a significant (p>0.05) increase with increased concentration of the oilfield wastewater when compared to the control. Other enzymes activities fluctuated around the control with LDH activity showing highest (2812.50±375.00 IU/L) response in all the organs. Lowest activity was recorded for ALT (4.00±0.00 IU/L) in the plasma. The changes observed can be used as an indicator of stress in C. gariepinus exposed to elevated levels of an oilfield wastewater. These changes can lead to death of fish and economic loss. The need therefore, for proper treatment of oilfield wastewater prior to discharge into the aquatic environment so as to reduce ecotoxicological problems and public health hazards is advocated.

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Evaluation of cytogenetic effects of lambda-cyhalothrin on Wistar rat bone marrow by gavage administration

Cited by 55 publications

References 29 publications

Altered canonical hedgehog-gli signalling axis in pesticide-induced bone marrow aplasia mouse model

Altered canonical hedgehog-gli signalling axis in pesticide-induced bone marrow aplasia mouse model

The in vitro genotoxic effects of a commercial formulation of α‐cypermethrin in human peripheral blood lymphocytes

Enzymatic responses of Clarias gariepinus (Burchell, 1822) exposed to sub-lethal concentrations of an oilfield wastewater

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