Evaluation of culture media for the production of secondary metabolites in a natural products screening program

Abstract

Variation in the growing environment can have significant impacts on the quantity and diversity of fungal secondary metabolites. In the industrial setting, optimization of growing conditions can lead to significantly increased production of a compound of interest. Such optimization becomes challenging in a drug-discovery screening situation, as the ideal conditions for one organism may induce poor metabolic diversity for a different organism. Here, the impact of different media types, including six liquid media and five solid media, on the secondary metabolite production of three fungal strains was examined in the context of the drug-discovery screening process. The relative production of marker compounds was used to evaluate the usefulness and reliability of each medium for the purpose of producing secondary metabolites.

New Dereplication Method Applied to NMR-Based Metabolomics on DifferentFusariumSpecies Isolated from Rhizosphere ofSenna spectabilis

“…[4][5][6][7][8] More recently, several other tools have been developed, aiming to achieve efficient and more factual paths for metabolite elucidation through analysis of high-resolution metabolite profiling. 7 Among the enhancements in the dereplication process, we highlight 2D 13 C NMR, high throughput screening (HTS), bioinformatics, MS/MS molecular networking applied to the omics sciences (genomics, proteomics, metabolomics), as well as chemometrics and other statistical tools, such as partial least-square discriminant analysis (PLS-DA), statistical total correlation spectroscopy (STOCSY). 6,7,[9][10][11] In parallel to the development of new dereplication tools, microorganisms, especially those isolated from unstudied NP matrices, such as plant's rhizosphere and marine organisms, appear as an interesting source of new active compounds, mainly due to their high molecular complexity and potential to produce heterogeneous secondary metabolites.…”

“…6,7,[9][10][11] In parallel to the development of new dereplication tools, microorganisms, especially those isolated from unstudied NP matrices, such as plant's rhizosphere and marine organisms, appear as an interesting source of new active compounds, mainly due to their high molecular complexity and potential to produce heterogeneous secondary metabolites. 12,13 Furthermore, microbes also possess the ability to use different solid substrates as a result of their biochemistry and biological evolution, enabling the survival in different environments through mutualistic association with plants and other organisms, allowing the production of different active metabolites in a single strain. 12,13 Senna spectabilis belongs to the Fabaceae family and refers to a plant widely used in folk herbal medicine and extensively studied towards their chemical diversity, majorly composed of pentacyclic triterpenes and piperidine alkaloids.…”

“…12,13 Furthermore, microbes also possess the ability to use different solid substrates as a result of their biochemistry and biological evolution, enabling the survival in different environments through mutualistic association with plants and other organisms, allowing the production of different active metabolites in a single strain. 12,13 Senna spectabilis belongs to the Fabaceae family and refers to a plant widely used in folk herbal medicine and extensively studied towards their chemical diversity, majorly composed of pentacyclic triterpenes and piperidine alkaloids.…”

Statistical optimization and valuable applications of bioactive compounds produced by marine Pseudoalteromonas piscicida

“…Such optimization becomes challenging in a drug-discovery screening situation (VanderMolen et al, 2013).…”

“…This result was almost in accordance with that reported by Nurul et al (2011). The bacteria belonging to genera Pseudoalteromonas were reported for their antioxidant activity (Velho-Pereira et al, 2015). Chemical characterization of the benzene extract Chemical composition of the benzene extract was determined using GC-MS.…”