Evaluation of Culex quinquefasciatus wings asymmetry after exposure of larvae to sublethal concentration of ivermectin

Alves, Stênio Nunes; Pujoni, Diego Guimarães Florêncio; Mocelin, Giovani; Melo, Alan Lane de; Serrão, J. E.

doi:10.1007/s11356-019-06963-5

Cited by 3 publications

(1 citation statement)

References 18 publications

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“…The larvae of C. quinquefasciatus used in this study were obtained in a semi-natural breeding place maintained at the Bioterium of the Biological Research Laboratory of the Federal Goiano Institute - Campus Urutaí (GO, Brazil), according to procedures recommended by Gerberg et al (1994) which were also used by Alves et al (2018) and Alves et al (2020) . Briefly, the females lay on plastic trays (50 cm × 40 cm x 25 cm) covered with Sombrite®60 % mesh, containing 25 L of deionized water and 20 g of feed used to feed mice.…”

Section: Methodsmentioning

confidence: 99%

Shedding light on toxicity of SARS-CoV-2 peptides in aquatic biota: A study involving neotropical mosquito larvae (Diptera: Culicidae)

Mendonça-Gomes

Charlie-Silva

Guimarães

et al. 2021

Environmental Pollution

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Knowledge about how the COVID-19 pandemic can affect aquatic wildlife is still extremely limited, and no effect of SARS-CoV-2 or its structural constituents on invertebrate models has been reported so far. Thus, we investigated the presence of the 2019-new coronavirus in different urban wastewater samples and, later, evaluated the behavioral and biochemical effects of the exposure of Culex quinquefasciatus larvae to two SARS-CoV-2 spike protein peptides (PSPD-2002 and PSPD-2003) synthesized in our laboratory. Initially, our results show the contamination of wastewater by the new coronavirus, via RT-qPCR on the viral N1 gene. On the other hand, our study shows that short-term exposure (48 h) to a low concentration (40 μg/L) of the synthesized peptides induced changes in the locomotor and the olfactory-driven behavior of the C. quinquefascitus larvae, which were associated with increased production of ROS and AChE activity (cholinesterase effect). To our knowledge, this is the first study that reports the indirect effects of the COVID-19 pandemic on the larval phase of a freshwater invertebrate species. The results raise concerns at the ecological level where the observed biological effects may lead to drastic consequences.

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Section: Methodsmentioning

confidence: 99%