Evaluation of cross-reactions in Histoplasma capsulatum serologic tests

Abstract

Cross-reactivity in Histoplasma serologic tests was evaluated by using sera from patients with histoplasmosis and other infections. Serum samples from 127 of 134 (95%) patients with histoplasmosis were judged positive by complement fixation tests, and 121 (90%) showed H bands, M bands, or both by immunodiffusion. Of these 134 patients, cross-reactions were seen to Blastomyces dermnaitidis in 53 patients (40%), to Coccidioides immitis

Evaluation of an elsa for the detection of anti‐histoplasma ribosomal and antihistoplasmin antibodies in histoplasmosis

“…Cross-reactivity was seen with both antigens (ribosomes, 50%; histoplasmin, 29%) in sera from patients with tuberculosis. Detectable levels of antibodies cross-reactive to Histoplusma antigens in tuberculosis have been previously reported by us (10) and others (23,29). The elevated levels of anti-Histoplusma reactivity observed in tuberculosis patients may be attributed to past or concurrent fungal infections, especially histoplasmosis and aspergillosis, which have been frequently reported as coinfections in patients with tuberculosis.…”

“…The elevated levels of anti-Histoplusma reactivity observed in tuberculosis patients may be attributed to past or concurrent fungal infections, especially histoplasmosis and aspergillosis, which have been frequently reported as coinfections in patients with tuberculosis. In our previous study, we presented data to support our hypothesis that, in addition to past or concurrent infection in tuberculosis patients, cross-reacting antigens between mycobacterium and Hi:stoptusma may also be responsible for serological crossreactions (10). Absorption of anti-H. cupsulutum antibodies with purified mycobacterial antigens decreased reactivity to histoplasmin in RIA, indicating the presence of antigens that are expressed by both M .…”

“…Crossreacticlns to H . cupsulutum antigens have been reported by others in blastomycosis (10,(22)(23)(24)26,27), coccidioidomycosis (10,(22)(23)(24)26), paracoccidioidomycosis (10,(23)(24)(25), cryptococcosis (10,25), candidiasis (lo), and aspergillosis (10). Cross-reactivity can be explained by the fact that fungi share several common cell wall antigens (28).…”

“…However, serologic cross-reactions occasionally create serious problems in the diagnosis of systemic fungal infections. The development of newer immunodiagnostic procedures like the enLyme-linked immunosorbent assay (ELISA) and radioimmunoassay (RIA) has enhanced the sensitivity of serodiagnosis of many fungal, bacterial, and viral infections (6)(7)(8)(9)(10).…”

Talaromyces marneffei laboratory cross reactivity with Histoplasma and Blastomyces urinary antigen

“…An initial laboratory test for endemic fungi can guide initial treatment towards early antifungal medication. However, crossreactivity between antigens in various fungal infection detection tests is well-documented, and cross reactions between Histoplasma and Blastomyces antigens are the most common (Wheat et al, 1986). Others have been described, such as that of Histoplasma antigen in patients with sporotrichosis (Assi et al, 2011).…”