Evaluation of copper-inducible fungal laccase promoter in foreign gene expression in Pichia pastoris

Kim, Sin-Il; Ha, Byung-Suk; Kim, Minseek; Park, Minsa; Ro, Hyeon-Su

doi:10.1007/s12257-015-0567-1

Cited by 5 publications

(4 citation statements)

References 26 publications

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“…There are two types of fungal laccase: constitutive laccase and inducible laccase. Most of the laccase found so far belong to inducible laccase, and their gene expression is prone to change with the environmental signal [58]. Many factors can regulate laccase genes transcription such as various aromatic compounds related to lignin derivatives, metal ions, nitrogen sources, and carbon sources, etc., which lead different transcriptional profiles not only among different species, but also within the same strain [59,60].…”

Section: Resultsmentioning

confidence: 99%

Optimization of Laccase from Ganoderma lucidum Decolorizing Remazol Brilliant Blue R and Glac1 as Main Laccase-Contributing Gene

Qin

Adil

et al. 2019

Molecules

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Many dyes and pigments are used in textile and printing industries, and their wastewater has been classed as a top source of pollution. Biodegradation of dyes by fungal laccase has great potential. In this work, the influence of reaction time, pH, temperature, dye concentration, metal ions, and mediators on laccase-catalyzed Remazol Brilliant Blue R dye (RBBR) decolorization were investigated in vitro using crude laccase from the white-rot fungus Ganoderma lucidum. The optimal decolorization percentage (50.3%) was achieved at 35 °C, pH 4.0, and 200 ppm RBBR in 30 min. The mediator effects from syringaldehyde, 1-hydroxybenzotriazole, and vanillin were compared, and 0.1 mM vanillin was found to obviously increase the decolorization percentage of RBBR to 98.7%. Laccase-mediated decolorization percentages significantly increased in the presence of 5 mM Na+ and Cu2+, and decolorization percentages reached 62.4% and 62.2%, respectively. Real-time fluorescence-quantitative PCR (RT-PCR) and protein mass spectrometry results showed that among the 15 laccase isoenzyme genes, Glac1 was the main laccase-contributing gene, contributing the most to the laccase enzyme activity and decolorization process. These results also indicate that under optimal conditions, G. lucidum laccases, especially Glac1, have a strong potential to remove RBBR from reactive dye effluent.

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Section: Resultsmentioning

confidence: 99%

Optimization of Laccase from Ganoderma lucidum Decolorizing Remazol Brilliant Blue R and Glac1 as Main Laccase-Contributing Gene

Qin

Adil

et al. 2019

Molecules

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“…Depending on the aim and on the required culture conditions, the promoter’s inducing agent has to be carefully evaluated. Some compounds, or the overexpressed product itself, might exert toxic effects in specific organisms ( 17 , 18 ). In other cases, the cost of the inducing substance could determine the fate of a promoter as not economically suitable for industrial production ( 19 , 20 ).…”

Section: Introductionmentioning

confidence: 99%

Modular Inducible Multigene Expression System for Filamentous Fungi

et al. 2022

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“…Thus, it must be considered that certain inductive or repressive substances or the produced protein has a toxic effect in some organisms (Kim et al 2016; Lee et al 2010). In addition, a leakiness of the promoter, which results in a minimal constitutive activity, leads to uncontrolled gene expression (Meyer et al 2011).…”

Section: Introductionmentioning

confidence: 99%

“…The selection of a suitable promoter system should be specifically adapted to experimental approaches because not every system brings along optimal conditions for the selected model organism. Thus, it must be considered that certain inductive or repressive substances or the produced protein has a toxic effect in some organisms (Kim et al 2016 ; Lee et al 2010 ). In addition, a leakiness of the promoter, which results in a minimal constitutive activity, leads to uncontrolled gene expression (Meyer et al 2011 ).…”

Section: Introductionmentioning

confidence: 99%

Inducible promoters and functional genomic approaches for the genetic engineering of filamentous fungi

Kluge

Terfehr

Kück

2018

Appl Microbiol Biotechnol

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In industry, filamentous fungi have a prominent position as producers of economically relevant primary or secondary metabolites. Particularly, the advent of genetic engineering of filamentous fungi has led to a growing number of molecular tools to adopt filamentous fungi for biotechnical applications. Here, we summarize recent developments in fungal biology, where fungal host systems were genetically manipulated for optimal industrial applications. Firstly, available inducible promoter systems depending on carbon sources are mentioned together with various adaptations of the Tet-Off and Tet-On systems for use in different industrial fungal host systems. Subsequently, we summarize representative examples, where diverse expression systems were used for the production of heterologous products, including proteins from mammalian systems. In addition, the progressing usage of genomics and functional genomics data for strain improvement strategies are addressed, for the identification of biosynthesis genes and their related metabolic pathways. Functional genomic data are further used to decipher genomic differences between wild-type and high-production strains, in order to optimize endogenous metabolic pathways that lead to the synthesis of pharmaceutically relevant end products. Lastly, we discuss how molecular data sets can be used to modify products for optimized applications.

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Evaluation of copper-inducible fungal laccase promoter in foreign gene expression in Pichia pastoris

Cited by 5 publications

References 26 publications

Optimization of Laccase from Ganoderma lucidum Decolorizing Remazol Brilliant Blue R and Glac1 as Main Laccase-Contributing Gene

Optimization of Laccase from Ganoderma lucidum Decolorizing Remazol Brilliant Blue R and Glac1 as Main Laccase-Contributing Gene

Modular Inducible Multigene Expression System for Filamentous Fungi

Inducible promoters and functional genomic approaches for the genetic engineering of filamentous fungi

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