Evaluation of Copan FecalSwab™ preserved stool specimens with the BD MAX™ Enteric Bacterial Panel and the BD MAX™ Extended Enteric Bacterial Panel

Rojas, Hanen Fernandez; Lima, Amorce; Kubasek, Carly; Gostnell, Alicia; Silbert, Suzane

doi:10.1016/j.diagmicrobio.2020.115055

Cited by 4 publications

(5 citation statements)

References 21 publications

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“…As we did not observe any loss in sensitivity for the FecalSwab as part of the clinical evaluation, we concluded 50 l of FecalSwab transport medium (Cary-Blair) to be the optimal sample volume for the BD Max EVP and EBP assays. These findings support those previously published for the BD Max EBP and extended EBP assays (9).…”

Section: Discussionsupporting

confidence: 93%

“…Despite having included some of the most common causes of acute diarrhea, we did not cover all pathogens that would be of clinical importance to isolate from FecalSwab specimens. Rojas et al ( 9 ) enhance our findings, as the authors evaluated the compatibility of the FecalSwab with additional pathogens ( Yersinia enterocolitica , enterotoxigenic Escherichia coli , Vibrio spp., and Plesiomonas shigelloides ) using the BD Max extended EBP panels; however, there are still several pathogens, especially intestinal parasites, that require investigation. Another limitation of our study is due to the probability that our findings may not be generalizable to all stool specimens, as we did not evaluate the role of host (microbiota, immune status, and antimicrobial treatment) and pathogen (pathogenicity, metabolism, etc.)…”

Section: Discussionsupporting

confidence: 54%

“…Lastly, the current study focuses on the performance of the FecalSwab in its compatibility with the BD Max system. Although our findings and those from others suggest that the FecalSwab can be used for enteric diagnostics with a range of platforms ( 6 , 8 , 9 ), volume standardization and clinical evaluation is required for laboratories using other nucleic acid amplification systems.…”

Section: Discussionmentioning

confidence: 74%

“…Copan FecalSwab collection devices have previously demonstrated equivalent performance to bulk stool for enteric pathogen detection using culture methods ( 7 ) and with the Cepheid Xpert ( 6 ), BioFire FilmArray ( 8 ) and BD Max ( 9 ) molecular platforms. However, this is the first report evaluating the FecalSwab with the BD Max system EVP assay.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of the FecalSwab for Stool Specimen Storage and Molecular Detection of Enteropathogens on the BD Max System

et al. 2020

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The FecalSwab™ system (Copan Italia, Brescia, Italy) is a convenient alternative to bulk stool for the diagnosis of enteric pathogens. Although U.S. Food and Drug Administration (FDA) approved for transport and culture of enteric bacterial pathogens, the FecalSwab™ has not been well assessed for its suitability with molecular platforms. In this study, we evaluated the FecalSwab™ as a specimen type for the BD MAX™ System using the viral and bacterial enteric panels (BD Diagnostics, Baltimore, USA). One-hundred eighty-six unpreserved stool specimens were collected and used to prepare matched bulk stool and FecalSwab™ samples. Performance was equivalent (P >0.48) to bulk stool for all targets when 50 μl of FecalSwab™ specimen was loaded onto the BD MAX™ assays. As stool specimens are often collected off-site from the clinical microbiology laboratory and require transport, we assessed the stability of stool specimens stored for up to 14 days at 40C, 220C, or 350C to account for varying transportation conditions. Molecular detection for the majority of viral targets (excluding astrovirus) was unaffected (ΔCT ≤1) by sample storage temperature over the two-week period; however, detection of enteric bacteria was variable if specimens were not refrigerated (220C or 350C). By demonstrating equivalent performance to matched bulk stool and maintaining molecular detection sensitivity when stored at 4°C, we suggest that the FecalSwab™ is a suitable specimen type for enteropathogen diagnostics on the BD MAX™ System.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 54%

Section: Discussionmentioning

confidence: 74%

Section: Discussionmentioning

confidence: 99%

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Evaluation of the FecalSwab for Stool Specimen Storage and Molecular Detection of Enteropathogens on the BD Max System

et al. 2020

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“…Stool specimen and rectal swabs are two types of preferred samples for rotavirus detection. Since long, Carry Blair transport medium has been the choice of transport medium for the rectal swab transportation to the laboratory at refrigerated 10,11,12,13 temperature.…”

Section: Introductionmentioning

confidence: 99%

Rotavirus Samples processing using Indigenously Developed Molecular Transport Medium (IMTM)

Nisa

Babar²,

Ali³

et al. 2023

L&S

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Objective: To perform comparative diagnostic study with rotavirus infected stool samples for efficacy ofindigenously prepared molecular transport medium (IMTM).Study Design: It was an experimental study.Place and Duration of Study: The study was conducted at Department of Microbiology of University of Karachi,Karachi Pakistan from June to July 2021.Materials and Methods: The current study was conducted based on hospital based surveillance of rotavirusgastroenteritis among children <5 years of age, stool samples were collected in commercially available viraltransport medium. Samples were known rotavirus positive /negative through PREMIER Rota clone Enzyme-Linked Immunosorbent Assay (ELISA) kit diagnostic test. Total 64 stool samples were selected to evaluate theefficacy of indigenous molecular transport medium (IMTM). Stool samples were spiked in IMTM andcommercial available viral transport medium (CVTM). RNA extraction and Real-time PCR were performed byQIAmp and in house assay respectively as per manufacturer' instructions. Similar method of RNA extractionand Real-Time PCR for both type of spiked samples was adopted.Results: Comparative analysis of Cycle threshold (Ct) values of paired samples (IMTM vs CVTM) was performed.Real-Time (RT-PCR) for rotavirus positive and negative results were consistent with each pair (p<0.0001).Conclusion: The indigenously formulated IMTM found capable for stool sample collection, transportation andprocessing for rotavirus detection through Real-time PCR.

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Microbial Etiologies and Clinical Characteristics of Children Seeking Emergency Department Care Due to Vomiting in the Absence of Diarrhea

Freedman

Xie

Lee

et al. 2021

Clinical Infectious Diseases

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Background As children with isolated vomiting are rarely able to provide a specimen suitable for routine pathogen testing, we have limited knowledge about their infecting pathogens. Methods Between December 2014 and August 2018, children <18 years old with presumed acute gastroenteritis who presented to two emergency departments (ED) in Alberta, Canada were recruited. Eligible participants had ≥3 episodes of vomiting and/or diarrhoea in a 24-hour period, <7 days of symptoms, and provided a rectal swab or stool specimen. We quantified the proportion of children with isolated vomiting in whom an enteropathogen was identified, and analysed clinical characteristics, types of enteropathogens, resources used and alternative diagnoses. Results Of the 2695 participants, at the ED visit, 295 (10.9%), 1321 (49.0%), and 1079 (40.0%) reported having isolated diarrhoea, vomiting and diarrhoea, or isolated vomiting, respectively. An enteropathogen was detected most commonly in those with vomiting and diarrhoea (1067/1321; 80.8%); detection did not differ between those with isolated diarrhoea (170/295; 57.6%) and isolated vomiting (589/1079; 54.6%; 95%CI of the difference: -3.4, 9.3). Children with isolated vomiting most often had a virus (557/1077; 51.7%), most commonly norovirus (321/1077; 29.8%); 5.7% (62/1079) had a bacterial pathogen. X-rays, ultrasounds, and urine tests were most commonly performed in children with isolated vomiting. Alternate aetiologies were most common in those with isolated vomiting (5.7%; 61/1079). Conclusions The rate of enteropathogen identification in children with isolated vomiting using molecular diagnostic tests and rectal swabs is substantial. Molecular diagnostics offer an emerging diagnostic strategy in children with isolated vomiting.

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Evaluation of Copan FecalSwab™ preserved stool specimens with the BD MAX™ Enteric Bacterial Panel and the BD MAX™ Extended Enteric Bacterial Panel

Cited by 4 publications

References 21 publications

Evaluation of the FecalSwab for Stool Specimen Storage and Molecular Detection of Enteropathogens on the BD Max System

Evaluation of the FecalSwab for Stool Specimen Storage and Molecular Detection of Enteropathogens on the BD Max System

Rotavirus Samples processing using Indigenously Developed Molecular Transport Medium (IMTM)

Microbial Etiologies and Clinical Characteristics of Children Seeking Emergency Department Care Due to Vomiting in the Absence of Diarrhea

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