Evaluation of continuous solvent extraction of organic acids from biological fluids

A GC/MS validated method for the nanomolar range determination of succinylacetone in amniotic fluid and plasma: An analytical tool for tyrosinemia type I

“…Very few methods have been used to extract SA from biological fluids prior to GC/MS analysis: one method requires a relatively long time span, while necessitating lyophilization and a liquid partition chromatography technique [13], whereas the others are based on liquid-liquid extraction [12,23,25]. Solvent extraction, on the other hand, is simple and we were able to confirm the efficiency of this known method [10,26] by comparing, in a triplicate assay, the peak areas of SA standards obtained after solvent extraction with those obtained after evaporation of pure oximated standards prepared in methanol (unextracted standards). The mean efficiency of extraction compared to the unextracted sample was 76% (data not shown).…”

R58fs Mutation in the HGD Gene in a Family with Alkaptonuria in the UAE

“…The organic layers were collected and dried using Savant speed vac plus SC110A with refrigerated vapor trap vacuum centrifuge system. Finally, organic acid residues were dissolved in pyridine and derivatization was performed with an equal volume of N,O‐Bis (trimethylsilyl) trifluroacetamide with 1% trimethyclorosilane (BSTFA) for 45 minutes at 95°C in a temperature block (Cohn et al 1978). The sample (1μl) was injected in the gas chromatograph/mass spectrometer (GC/MS) using the splitless mode.…”