Evaluation of commercially available immuno-magnetic agglutination and enzyme-linked immunosorbent assays for rapid point-of-care diagnostics of COVID-19

Abstract

Introduction: Coronavirus Disease 2019 (COVID-19) is caused by severe acute respiratory coronavirus-2 (SARS-CoV-2). Fast, accurate and simple blood-based assays for quantification of anti-SARS-CoV-2 antibodies are urgently needed to identify infected individuals and keep track of the spread of disease. Methods: The study included 35 plasma samples from 22 individuals with confirmed COVID-19 by real time reverse transcriptase polymerase chain reaction and 40 non COVID-19 plasma samples. Anti-SARS-CoV-2 IgM/IgA or IgG antibodies were detected by a microfluidic quantitative immunomagnetic assay (IMA)(ViroTrack Sero COVID IgM+IgA/IgG Ab, Blusense Diagnostics, Denmark) and by enzyme-linked immunosorbent assay ((ELISA) (EuroImmun Medizinische Labordiagnostika, Germany). Results: Of the 35 plasma samples from the COVID-19 patients, 29 (82.9%) were positive for IgA/IgM or IgG by IMA and 29 samples (82.9%) were positive by ELISA. Sensitivity for only one sample per patient was 68% for IgA+IgM and 73% IgG by IMA and 73% by ELISA. For samples collected 14 days after symptom onset, the sensitivity of both IMA and ELISA was around 90%. Specificity of the IMA reached 100% compared to 95% for ELISA IgA and 97.5% for ELISA IgG. Conclusion: IMA for COVID-19 is a rapid simple-to-use point of care test with sensitivity and specificity similar to a commercial ELISA.

Point-of-care testing detection methods for COVID-19

“…Maria Engel Moeller et al have evaluated the BluBox system with ELISA method, in 35 plasma samples from COVID-19 patients, 29 could be detected by BluBox. 165 And for samples collected 14 days after symptom appear, the sensitivity of both BluBox system and ELISA was around 90%, specificity of BluBox system could reach 100%, while ELISA IgA was 95%.…”

Biosensing on the Centrifugal Microfluidic Lab-on-a-Disc Platform

“…This trend is expected to grow in the next coming years. BluSense Diagnostics have already reached commercial success by offering a centrifugal style chip requiring one droplet of blood to carry out the disease diagnostics tests on their custom centrifuge analyser system [215].…”