Point-of-care testing detection methods for COVID-19

Song, Qi; Sun, Xindi; Dai, Zhenhong; Gao, Yibo; Gong, Xiuqing; Zhou, Boyu; Wu, Jinbo; Wen, Weijia

doi:10.1039/d0lc01156h

Cited by 163 publications

(136 citation statements)

References 141 publications

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“…Loop-mediated isothermal amplification (LAMP) [ 15 ] and recombinase polymerase amplification (RPA) can detect the lowest copy number of nucleic acids. Real-time PCR for POCT has been commercialized [ 16 ] to process a single sample or multiple samples, up to 80 samples [ 17 ]. The topic of POC-PCR assays for COVID-19 detection was recently reviewed by Gupta et al [ 18 ].…”

Section: Pcr Technology and Immunoassaysmentioning

confidence: 99%

“…Commercial POC immunoassays can detect viral antigens (specific monoclonal antibodies are used to bind the SARS-CoV-2 antigens: N and S proteins), total antibody, and IgM/IgG antibodies. Detection techniques are mainly based on colloidal gold/immunofluorescence chromatography (lateral flow immunoassay, LFA), enzyme-linked immunosorbent assay, and chemiluminescence [ 16 ].…”

Section: Pcr Technology and Immunoassaysmentioning

confidence: 99%

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Perspectives on electrochemical biosensing of COVID-19

Luong

Buzid

Vashist³

et al. 2021

Current Opinion in Electrochemistry

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Rapid detection of human coronavirus 2019, termed as SARS-CoV-2 or COVID-19 infection is urgently needed for containment strategy due to its unprecedented spreading. Novel biosensors can be deployed in remote clinical settings without central facilities for infection screening. Electrochemical biosensors serve as analytical tools for rapid detection of viral structure proteins, mainly spike (S) and nucleocapsid (N) proteins, human immune responses, reactive oxygen species (ROS), viral ribonucleic acid, polymerase chain reaction byproducts, and other potential biomarkers. The development of point of care testing (POCT) devices is challenging due to the requirement of extensive validation, a time-consuming and expensive step. Together with specific biorecognition molecules, nanomaterials enabled-based biosensors have emerged for the fast detection of early viral infections.

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Section: Pcr Technology and Immunoassaysmentioning

confidence: 99%

Section: Pcr Technology and Immunoassaysmentioning

confidence: 99%

Perspectives on electrochemical biosensing of COVID-19

Luong

Buzid

Vashist³

et al. 2021

Current Opinion in Electrochemistry

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“… Detection: The current standard method for detection is the reverse transcription-polymerase chain reactor (RT-PCR); the analysis detects the presence of viral nucleic acids, uses repeated cycles of heating and cooling to make many copies of DNA, however, it requires complex equipment, requires at least 4−6 hours to complete and it is expensive. Therefore, Point-of-care testing (POCT) is advantageous( Song et al, 2021 ). Loop-mediated isothermal amplification (LAMP) is similar to RT-PCR but the difference is that the process is carried out at one temperature (60 to 65 °C) making the detection quicker, easy to use, and do not require expensive reagents or instruments ( Thompson and Lei, 2020 ).…”

Section: Sars-cov-2 As a Warning Signmentioning

confidence: 99%

“…Biosensors can also be especially useful in the detection of the virus in aquatic compartments since they are analytical devices with biological elements that generate physicochemical signals, and according to recent studies, they have great advantages such as the use of small sample quantities, cost-effectiveness, and prompt response, The detection of COVID-19 on time, could decrease the transmission of the disease providing accurate information that would help considerably the eradication( Mao et al, 2020 ; Song et al, 2021 ). More information can be found in the article “Point-of-care testing detection methods for COVID-19” ( Song et al, 2021 )…”

Section: Sars-cov-2 As a Warning Signmentioning

confidence: 99%

Emerging contaminants, SARS-COV-2 and wastewater treatment plants, new challenges to confront: A short review

Pacheco

Hilares

Andrade

et al. 2021

Bioresource Technology Reports

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The current pandemic caused by SARS-CoV-2 has put public health at risk, being wastewater-based epidemiology (WBE) a potential tool in the detection, prevention, and treatment of present and possible future outbreaks, since this virus enters wastewater through various sources such as feces, vomit, and sputum. Thus, advanced technologies such as advanced oxidation processes (AOP), membrane technology (MT) are identified through a systematic literature review as an alternative option for the destruction and removal of emerging contaminants (drugs and personal care products) released mainly by infected patients. The objectives of this review are to know the implications that the new COVID-19 outbreak is generating and will generate in water compartments, as well as the new challenges faced by wastewater treatment plants due to the change in a load of contaminants and the solutions proposed based on the aforementioned technologies to be applied to preserve public health and the environment.

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“…be performed by non-laboratory professionals [22,23]. Recently, the COVID-19 pandemic has shined a spotlight on Clinical and Laboratory Improvement Amendments (CLIA)-waived diagnostic testing.…”

mentioning

confidence: 99%

Detection of Sars-Cov-2At the Point of Care

Loeffelholz¹,

Tang

2021

Bioanalysis

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Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the etiologic agent of COVID-19. Testing for SARS-CoV-2 infection is a critical element of the public health response to COVID-19. Point-of-care (POC) tests can drive patient management decisions for infectious diseases, including COVID-19. POC tests are available for the diagnosis of SARS-CoV-2 infections and include those that detect SARS-CoV-2 antigens as well as amplified RNA sequences. We provide a review of SARS-CoV-2 POC tests including their performance, settings for which they might be used, their impact and future directions. Further optimization and validation, new technologies as well as studies to determine clinical and epidemiological impact of SARS-CoV-2 POC tests are needed.

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Point-of-care testing detection methods for COVID-19

Abstract: COVID-19 is an acute respiratory disease caused by SARS-CoV-2, which has high transmissibility. People infected with SARS-CoV-2 can develop symptoms including cough, fever, pneumonia and other complications, which in severe...

Cited by 163 publications

References 141 publications

Perspectives on electrochemical biosensing of COVID-19

Perspectives on electrochemical biosensing of COVID-19

Emerging contaminants, SARS-COV-2 and wastewater treatment plants, new challenges to confront: A short review

Detection of Sars-Cov-2At the Point of Care

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