Evaluation of combined disc method for the detection of metallo-β-lactamase producing Gram negative bacilli

Omair, Muhammad; Usman, Javaid; Kaleem, Fatima; Hassan, Ali; Khalid, Ali; Fahim, Qanita

doi:10.21161/mjm.33911

Cited by 10 publications

(9 citation statements)

References 9 publications

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“…We found that all isolates found to be MBL positive by CDT were also positive with the E Test and one isolate which were positive by E Test, were found to be MBL negative by CDT. These findings are consistent with the study done by Maria Omair et al 17 The CDT detected 48.2% of MBL producers among which were negative by the DDST and DPT. This may be because the sensitivity varies with the distance between the discs and the antibiotic discs chosen for the test.…”

Section: Discussionsupporting

confidence: 93%

Detection of metallobetalactamase producing imipenem resistant acinetobacter species in intensive care unit patient in a Tertiary Care Centre

Vinoba¹,

Rashmi²

2020

IJMR

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Introduction: Metallobetalactamase producing Acinetobacter species has been reported to be an important cause of nosocomial infection and is a critical therapeutic problem worldwide, especially in the intensive care unit. Objectives: To determine the frequency of metallo-β-lactamases among imipenem-resistant Acinetobacter species and to compare different phenotypic methods. Materials and Methods: 59 imipenem-resistant Acinetobacter species isolated from various clinical samples were tested for metallo-β-lactamase production using different phenotypic methods. Minimal Inhibitory Concentratrion (MIC) to meropenem was determined by E test. Results: Of all the imipenem resistant isolates, 50.8% of Acinetobacter species were MBL producers. MIC of all those MBL producing isolates were ≥ 16μg/ml. Among the Acinetobacter species MBL production were detected in 30 isolates (50.8%) by E test, 29 isolates (49.2%) by CDT and in 15 isolates (25.4%) by DDST and DPT. The sensitivity and specificity of CDT, DDST and DPT compared to E test was 98%, 48.1%, 48.1% and 100%, respectively. Conclusion: Prevalence of MBL producing Acinetobacter species is being increasingly reported in ICU patients. The MIC of all the MBL producing isolates for meropenem were >16μg/ml (Resistant). E test and CDT were more reliable for MBL detection. CDT was cost effective, easy to perform and interpretation also straightforward. MBL producing isolates were multidrug resistant making therapeutic choices limited. Continuous antibiotic surveillance, infection control practices and an effective antibiotic policy are required to address the problem of MBL -associated infections.

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Section: Discussionsupporting

confidence: 93%

Detection of metallobetalactamase producing imipenem resistant acinetobacter species in intensive care unit patient in a Tertiary Care Centre

Vinoba¹,

Rashmi²

2020

IJMR

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“…The resistance to these compounds is mediated by a combination of different mechanisms; with enzymatic hydrolysis, mediated by carbapenemases being the most important mechanism of resistance. Carbapenem hydrolyzing enzymes can be divided into two main sub types; Serine β-lactamases (Ambler class A and D) and metallo-β-lactamases (MBLs) (Ambler class B) [6].…”

Section: Introductionmentioning

confidence: 99%

“…Metallo-β-lactamases are one of the most worrisome resistance mechanisms because in addition to limiting treatment options, their genes are carried on highly mobile elements, allowing easy dissemination [6]. Strains producing MBLs are recorded to be responsible for prolonged HAIs outbreaks associated with serious infections [4]; together with higher morbidity and mortality [7].…”

Section: Introductionmentioning

confidence: 99%

The Increased Frequency of Carbapenem Resistant Non Fermenting Gram Negative Pathogens as Causes of Health Care Associated Infections in Adult Cancer Patients

El-Mahallawy¹,

Hamid²,

Hassan³

et al. 2015

JCT

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Background and Aim: Multi drug resistant Non fermenting gram negative bacilli (NFGNB) have emerged as a major cause of health-care associated infections especially in immunocompromised hosts. The aim of the study was to investigate the prevalence of NFGNB as a cause of health-care associated infections (HAI) in cancer patients and determine their resistance pattern. Patients and Methods: During the study period, 158 NFGNB isolates were collected. Microscan Walk Away 9 was used for identification and testing for the metallo-β-lactamases (MBLs) was done by Imipenem-EDTA combined disk synergy test (CDST-IPM). Results: NFGNB represented 29.0% of infections caused by gram negative organisms. Carbapenem resistance, the multi-drug resistant (MDR) phenotype, and MBL production were documented in 70%, 63%, and 59% of NFGNB isolates, respectively. MDR-NFGNB rates were significantly higher among hospitalized patients, medical department and those with longer duration of hospital stay (p = 0.034, 0.026, 0.019; respectively) than non MDR-NFGNB. Conclusion: A high level of carbapenem and multi-drug resistance were detected among the non-fermenter pathogens isolated from hospitalized cases and were more frequently encountered in high risk adult cancer patients requiring longer duration of hospitalization. The MDR-NFGNB are constituting important causes of health-care associated infections in cancer patients.

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“…Another probable explanation mightbe the susceptibility of some bacterial strains to EDTA, which could affect permeability of bacterial membrane, resulting in false positive results of phenotypic tests used for the MβLs detection (Karthika et al, 2009;Aktas and Kayacan, 2008). In general, phenotypic method, especially DDST is reported to be sensitive for detection of MβLs due to wide genetic varieties among gram negative bacilli isolated from clinical samples (Omair et al, 2012).…”

Section: Resultsmentioning

confidence: 99%

Phenotypic Detection and Genotypic Characterization of Metallo-Beta-Lactamases in Gram Negative Bacilli isolated from Mansoura University Hospital in Egypt

Zaki¹,

Mashaly²,

Agamy³

2017

Int.J.Curr.Microbiol.App.Sci

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Evaluation of combined disc method for the detection of metallo-β-lactamase producing Gram negative bacilli

Cited by 10 publications

References 9 publications

Detection of metallobetalactamase producing imipenem resistant acinetobacter species in intensive care unit patient in a Tertiary Care Centre

Detection of metallobetalactamase producing imipenem resistant acinetobacter species in intensive care unit patient in a Tertiary Care Centre

The Increased Frequency of Carbapenem Resistant Non Fermenting Gram Negative Pathogens as Causes of Health Care Associated Infections in Adult Cancer Patients

Phenotypic Detection and Genotypic Characterization of Metallo-Beta-Lactamases in Gram Negative Bacilli isolated from Mansoura University Hospital in Egypt

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