Evaluation of circulating TP53 antigen in the sera of gastric cancer patients: As a tumor marker

Nasif, Wesam A.; Mukhtar, Mohammed Hasan; El-Emshaty, Hoda; Albukhari, Talat A.

doi:10.1016/j.bgm.2013.10.001

Cited by 5 publications

(3 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In detail, if 30% or more of the malignant nuclei were positive, the tumour was scored as positive, while if fewer than 30% of the nuclei were stained, the tumour was scored as negative (Fig. 1) [78][79][80]. For study purposes, right-sided and left-sided colorectal primary tumours were defined as proximal or distal to the splenic flexure.…”

Section: Methodsmentioning

confidence: 99%

The Role of p53 Expression in Patients with RAS/BRAF Wild-Type Metastatic Colorectal Cancer Receiving Irinotecan and Cetuximab as Later Line Treatment

et al. 2021

View full text Add to dashboard Cite

Background Preclinical and clinical data indicate that p53 expression might modulate the activity of the epidermal growth factor receptor (EGFR), influencing response/resistance to anti-EGFR monoclonal antibodies. However, the association between p53 status and clinical outcome has not been clarified yet. Objective In our study, we evaluated the role of p53 expression in patients with RAS/BRAF wild-type metastatic colorectal cancer (mCRC) receiving irinotecan/cetuximab in an exploratory and a validation cohort. Patients and Methods p53 expression was analysed in patients with RAS/BRAF wild-type mCRC receiving second-line or third-line irinotecan/cetuximab. Survival distribution was assessed by the Kaplan-Meier method, while the log-rank test was used for survival comparison. Results Among 120 patients with RAS/BRAF wild-type mCRC included in our analysis, 52 (59%) and 19 (59%) patients showed p53 overexpression in the exploratory and validation cohort, respectively. In the exploratory cohort, low p53 expression was correlated with better median progression-free survival (hazard ratio 0.39; p < 0.0001), median overall survival (hazard ratio: 0.23; p < 0.0001) and response rate (p < 0.0001). These results were confirmed by data of the validation cohort where we observed better median progression-free survival (hazard ratio: 0.48; p = 0.0399), median overall survival (hazard ratio: 0.26; p = 0.0027) and response rate (p =0.0007) in patients with p53 normal expression mCRC. Conclusions In our study, p53 overexpression was associated with anti-EGFR treatment resistance in patients with RAS/ BRAF WT mCRC, as confirmed in a validation cohort. Larger studies are needed to validate the role of p53 and investigate EGFR cross-talk in these patients.Pina Ziranu and Eleonora Lai contributed equally to this work.

show abstract

Section: Methodsmentioning

confidence: 99%

The Role of p53 Expression in Patients with RAS/BRAF Wild-Type Metastatic Colorectal Cancer Receiving Irinotecan and Cetuximab as Later Line Treatment

et al. 2021

View full text Add to dashboard Cite

show abstract

“…TP53 mutation and overexpression has been noted in over 50% of gastric cancer cases (Forbes et al, 2011). A study by Nasif et al (2013), evaluated circulating TP53 antigen in the sera of gastric cancer patients as a tumor marker. This study detected TP53 expression in 62.5% of the nuclei in carcinoma cells in patients, thus concluding P53 concentration to be a useful marker for gastric cancer (see Fig.…”

Section: Scenario In Saudi Arabiamentioning

confidence: 99%

Gastric cancer & prospects of cancer in Saudi Arabia peninsula

Abuderman

2019

Saudi Journal of Biological Sciences

View full text Add to dashboard Cite

Gastric cancer is classified to be an aggressive disease with poor treatment outcome, as most cases remain undetected until later stages, wherein surgery and few chemotherapeutics become the only recommended treatment course. The process of cancer development is multistep involving many stages and types of precancerous lesions, and hence, routine monitoring becomes a necessity in those detected with these or exposed to risk factors. Studying the pattern of gastric cancer for any geographical region is also important to control mortality and focus on implementation of efficient management and treatment guidelines. The cause for gastric cancer can be genetic, racial as well as environmental, and hence the pattern of this malignancy differs across geographical regions and between the developing and the developed nations. In case of the Kindgom of Saudi Arabia, very few hospital-based reports have been published highlighting the pattern of gastric cancer, and the associated incidence and mortality rates. However, classified to be one of the most crucial cancer forms in Saudi Arabia, research pertaining to epidemiology, presentation and pathological features are limited. Studying gastric cancer occurrence from public health viewpoint is important also because eradication of causative agents like those that H. pylori has also shown been not reduce the risk of cancer development among individuals with atrophic metaplastic gastritis. In case of Saudi Arabia, many inherent risks for this malignancy exists like waterpipe smoking and shift in diet pattern from the traditional Mediterranean diet. Our review focusses on pattern of gastric cancer on a global scale in comparison to scenario in Saudi Arabia. The aim is to encompass all of the less stressed upon facts about this malignancy in the Kingdom, paving way for future work in this regards.

show abstract

“…Como observado nos experimentos de QCM, a presença de uma maior concentração de NPs disponibiliza uma maior área superficial para a imobilização de anticorpos e, consequentemente, promove a presença de maior número de sítios de ligação para interação com a proteína. 136 Este aumento do intervalo linear é importante, pois permite a detecção de concentrações de p53 com uma relevância clínica para o diagnóstico de várias doenças, incluindo-se câncer gástrico (420 pg mL −1 ), 137 carcinoma pancreático (50 pg mL −1 ) e câncer de ovário (200 pg mL −1 ). 138 Além disto, o uso de uma maior concentração de NPs também influenciou de maneira positiva na repetibilidade de fabricação dos imunossensores, que foi avaliada a partir de voltammograms de pulso diferencial obtidos para 5 eletrodos ,0 × 10 −3 mol L −1 em tampão fosfato 0,1 mol L −1 pH 7.…”

Section: Detecção De P53 Em Soluções Padrãounclassified

Novas estratégias de fabricação de imunossensores sem marcação à base de eletrodos impressos de carbono para a detecção de biomarcadores de câncer

Redin¹

View full text Add to dashboard Cite

Label-free electrochemical immunosensors based on screen-printed carbon electrodes are a promising tool for performing fast, simple and economical detection of cancer biomarkers.However, despite the potential of these devices, some limitations, such as their low sensitivity, need to be overcome before their widespread use as technology for clinical analysis. Herein, we proposed different strategies to modify screen-printed carbon electrodes, aiming to improve the analytical signal and the surface area for the immobilization of antibodies, which are two essential parameters for reaching good analytical performance in label-free immunosensors. We explored the application potential of carbon black and NiFe 2 O 4 nanoparticles, two low-cost nanomaterials with excellent electrochemical properties, which remain relatively unexplored in the construction of electrochemical immunosensors. In a first approach, an immunosensor was developed for detecting the carbohydrate antigen CA 19-9 using electrodes modified with composites of carbon black and the polyelectrolytes polyethyleneimine and polyacrylic acid. The immunosensor exhibited a low detection limit of 0.07 U mL − 1 , in addition to an excellent fabrication reproducibility, stability and selectivity.The proposed device showed good correlation with commercial immunoassays in the determination of CA 19-9 in cell lysates and human serum samples from pancreatic cancer patients. An immunosensor for detecting the tumor protein p53 was also developed, using electrodes modified with films of polyethyleneimine and NiFe 2 O 4 nanoparticles. The analytical parameters of the devices are among the best reported in the literature for determination of p53, with a low detection limit of 5 fg mL −1 . Also, the immunosensors showed an excellent selectivity toward p53 detection in fetal bovine serum, saliva and cell lysate samples. The platforms proposed herein combined the numerous advantages of screenprinted electrodes with the excellent properties of the studied nanomaterials, allowing the development of low-cost (˂BRL$ 4/ test) and disposable devices with excellent analytical performance, which can be adapted for the detection of several disease markers.

show abstract

Evaluation of circulating TP53 antigen in the sera of gastric cancer patients: As a tumor marker

Cited by 5 publications

References 23 publications

The Role of p53 Expression in Patients with RAS/BRAF Wild-Type Metastatic Colorectal Cancer Receiving Irinotecan and Cetuximab as Later Line Treatment

The Role of p53 Expression in Patients with RAS/BRAF Wild-Type Metastatic Colorectal Cancer Receiving Irinotecan and Cetuximab as Later Line Treatment

Gastric cancer & prospects of cancer in Saudi Arabia peninsula

Novas estratégias de fabricação de imunossensores sem marcação à base de eletrodos impressos de carbono para a detecção de biomarcadores de câncer

Contact Info

Product

Resources

About