Evaluation of chitosan gel with 1% silver sulfadiazine as an alternative for burn wound treatment in rats

Abstract

Purpose: Evaluation of the rheological, biological and therapeutic properties of a new topical formulation consisting of chitosan gel containing 1% silver sulfadiazine, as an alternative for the treatment of burn wounds. Methods: An experimental study was done with 21 Wistar rats divided into three groups. Group I was treated with chitosan gel without the antimicrobial, group II was treated with chitosan gel with 1% silver sulfadiazine and group III was treated with commercially available 1% silver sulfadiazine cream. Results: Due to its pseudoplastic characteristic and good bioadhesiveness, the chitosan gels showed a satisfactory retention time over the wounds. No statistical difference was found in the amount of drug released from the chitosan gel and commercially available cream, as well as in the healing time among the groups. Wounds treated with chitosan gel with silver sulfadiazine showed a higher fibroblast production and a better angiogenesis than in the other groups, which are important parameters on the evolution of the healing process. Conclusion: The topical use of chitosan gel in association with silver sulfadiazine ameliorated the neovascularization and inflammatory reaction in burn wounds. This new formulation showed advantageous rheological properties and efficient release of the drug. Key words: Chitosan. Silver Sulfadiazine. Gel. Wound Healing. Rats. RESUMOObjetivo: Avaliar as propriedades reológicas, biológicas e terapêuticas de uma nova formulação de uso tópico, a partir de um gel de quitosana, contendo sulfadiazina de prata a 1%, no tratamento de queimaduras. Métodos: Foi realizado estudo experimental com 21 ratos Wistar distribuídos em três grupos de sete animais. As queimaduras dos animais do grupo I foram tratadas com gel de quitosana sem antimicrobiano, o grupo II foi tratado com gel de quitosana contendo sulfadiazina de prata 1% e o grupo III foi tratado com creme de sulfadiazina de prata 1%, comumente utilizado no tratamento de queimados. Resultados: Devido às características pseudoplásticas e à boa bioadesividade, os géis de quitosana apresentaram um tempo de retenção satisfatório sobre as feridas. A liberação da sulfadiazina de prata, bem como o tempo de cicatrização, não foram estatisticamente diferentes. Feridas tratadas com o gel de quitosana contendo sulfadiazina de prata apresentaram uma maior produção de fibroblastos e uma melhor angiogênese, comparando-se com os outros grupos, fatores que indicaram uma maior evolução no processo de cicatrização. Conclusão: O uso tópico do gel de quitosana com sulfadiazina de prata a 1% melhorou a neovascularização e a reação inflamatória em queimaduras e essa nova formulação mostrou boas propriedades reológicas associadas a eficiente liberação do fármaco. Descritores: Quitosana. Sulfadiazina de prata. Cicatrização de Feridas. Ratos.

Silver Sulfadiazine Nanosystems for Burn Therapy

“…Diffusion through a dialysis membrane is a widely used technique to evaluate the release from colloidal dispersions and topical formulations (21,22). An SSD marketed formulation contains 1% w/w SSD.…”

“…In order to compare release profiles of 0.5% SSD nanogels with marketed formulation, 1% marketed cream was diluted with Carbopol gel to 0.5%. Nascimento et al have reported the use of a dialysis bag to determine SSD release from chitosan gels and marketed cream (22). PBS was used as a medium to analyze SSD release from SSD chitosan wound dressings (31).…”

Curcumin-loaded Chitosan Tripolyphosphate Nanoparticles as a safe,natural and effective antibiotic inhibits the infection of Staphylococcusaureus and Pseudomonas aeruginosa in vivo

“…Chitosan could accelerate coagulation and enhance the functions of inflammatory cells (13)(14)(15)(16). Moreover, it has been reported that chitosan could increase the tensile strength of wounds (17).…”

Biopolymers as Wound Healing Materials: Challenges and New Strategies

“…Due to its pseudoplastic characteristic and high bioadhesiveness, the chitosan gels with and without silver sulfadiazine showed a satisfactory retention time over the wounds. Wounds treated with chitosan gel with silver sulfadiazine showed a higher fibroblast production and a better angiogenesis than that of commercially available silver sulfadiazine cream, which are important parameters on the evolution of the healing process (Nascimento et al, 2009). Polyvinyl alcohol hydrogels with different combinations, prepared with chitosan and dextran, are also used as wound dressing material.…”