Evaluation of chemosterilant damage to the testes of the housefly, Musca domestica, by microscopic observation and by measurement of the uptake of 14C-compounds

Cline, Richard E.

doi:10.1016/0022-1910(68)90005-x

Cited by 8 publications

(3 citation statements)

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“…A similar effect, together with a general reduction in the size of the testes was observed by Schwartz (1965) when Hippelates fed on 1% metepa and tepa contained in sugar water. Cline (1968) applied 0-3% thiotepa to the diet of house-flies. After four days on the treated diet, whole mounts of the testes stained by the Feulgen method indicated that a marked reduction in spermatogonia had occurred as a result of treatment, although the structure of the testes as a whole remained intact.…”

Section: Gross Effects Of Chemosterilants On the Insect Reproductive mentioning

confidence: 99%

Insect chemosterilants: a review

Campion¹

1972

Bull. Entomol. Res.

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In this paper the present status of insect chemosterilants is reviewed. Chemosterilants may be categorised into eight main chemical groups. Practical control measures involving the use of chemosterilants are discussed, also the persistence of these substances when applied to the insect. The gross effects of chemosterilants on insect reproductive systems and also the effects on egg develpment when female insects are mated to chemosterilised males are described, including dominant lethality, reciprocal translocation, enzyme inhibition and sperm inactivation. The possibilities of recovery of fertility and insect resistance to the sterilising effects of chemosterilants are discussed. Endocrine misfunction and chemosterilant action are related. The only practical chemosterilants as control agents are the aziridines although mutagenic hazards associated with these substances preclude their widespread usage. The sterilising activity of certain insect hormone analogues and related substances encourages the belief that safe insect specific sterilising agents can be found.

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Section: Gross Effects Of Chemosterilants On the Insect Reproductive mentioning

confidence: 99%

Insect chemosterilants: a review

Campion¹

1972

Bull. Entomol. Res.

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“…The degeneration and the decrease of the volüme of ovaries and testes caused by chemosterilants were shown on Blatella germanica (SMITTLE et al, 1966), on Musca domestica (MORGAN, 1967), on Oncopeltus fasciatus (ECONOMOPOULOS, 1971) and on Trichoplucia ni (HENNEBERRY et al, 1972). With the effect of chemosterilant, the vacuolisation of cytoplasm and nucleus, the aggregation of chromatin, the aberation of chromosome and the dififerentiation of celi content of different parts of reproductive organs occured (LANDA Ytnd REZABOVA, 1965;CLINE, 1968;LaCHANCE and LEVER-ICH, 1968;KLASSEN et al, 1969;SANENA andADITYA, 1969, 1974;WILSON and HAYS, 1969;ADDY, 1970;LANDA and MATOLIN, 1971;NATH and SHEIKHER, 1976).…”

Section: Introductionmentioning

confidence: 99%

The Effects of Thiotepa on the Fine Structure of the Ovarium of the Last Instar Larvae of Agrotis Ipsilon (Hufnagel) (Lepidoptera Noctuidae)

Suludere¹,

Karol²

1986

Communications, Faculty of Science, University of Ankara Series C Biology, Geological Engineering and Geophysical Engineering

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Thiotepa was applied at two doses (0.01 % and 0.1 %) and for 3 or 10 minutes to the abdomens of A. ipsilon larvae.The coating layers of ovaries are distinctly affected by 0.1 % Thiotepa for each period. The cells in the ovarioles shovv, also, degenerations after using 0.01 % and 0.1 % Thiotepa. 0.01 % Thiotepa application for three minutes has a less effect. The increase in concentration as iveli as the duration of application of Thiotepa increases also the degeneration more severely in the cells of ovarioles. A complete destruction is obtained after the application of 0.01 % Thiotepa for 10 minutes.The apperances of these degenerations are shown ıvith electronraicrographs in detail Thiotepa is a vcry effective chemosterialant for A. ipsilon.

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“…While the cumulative effect of these compounds lead to sterility their mode of action at cellular level is not yet clear. Many chemosterilants belonging to aziridinyl compounds, phosphoramides and s-tri azines are known to cause cytogenetic damage in the reproductive or embryonic tissues of insects (LaChance et al 1968, Morgan 1967, Cline 1968, Reinecke et al 1969). In addition, a similar damage to the brain cells of Aedes aegypti (L.) has been observed by larval treatment of apholate and hempa (Rai 1964, Sharma and Rai 1969, George and Brown 1967.…”

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confidence: 99%

Cytogenetic Basis of Chemically-Induced Sterility in <i>Culex pipiens fatigans</i> Wiedemann

1973

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Studies on the potential use of induced sterility in insect control have resulted in the discovery of a large number of chemicals having effect on fertility and fecun dity of many species of insects. While the cumulative effect of these compounds lead to sterility their mode of action at cellular level is not yet clear. Many chemosterilants belonging to aziridinyl compounds, phosphoramides and s-tri azines are known to cause cytogenetic damage in the reproductive or embryonic tissues of insects (LaChance et al. 1968, Morgan 1967, Cline 1968, Reinecke et al. 1969). In addition, a similar damage to the brain cells of Aedes aegypti (L.) has been observed by larval treatment of apholate and hempa (Rai 1964, Sharma and Rai 1969, George and Brown 1967. As a result of these studies a concept has evolved that cytogenetic damage caused by chemicals is some how related to sterility (LaChance et a1. 1968).In our earlier studies, a number of alkylating and non-alkylating chemosteri lants were shown to induce sterility in Culex pipiens fatigans Wied. . The present investigations were, therefore, aimed to elucidate a cytogenetic basis of such chemosterilant-induced sterility in C. p. fatigans by detailed studies involving their effects on somatic, reproductive and embryonic tissues, and on DNA synthesis in the gonads. The present paper deals with the effects of apholate, metepa and hempa on the somatic chromosomes. Materials and methodsThe mosquitoes for the present studies were drawn from a Delhi strain of C. p. fatigans established in our laboratory since 1965 (Pillai and Grover 1969). The chemosterilants employed were as follows: 1) Apholate-2, 2, 4, 4, 6, 6-hexahydro-2, 2, 4, 4, 6, 6-hexakis (1-aziridinyl)-1, 3, 5, 2, 4, 6 triazatriphosphorine, 2) Metepa-tris (2-methyl-l-aziridinyl) phosphine oxide, and 3) Hempa-Hexamethyl phosphoric triamide.Mid-forth instar larvae (65 hrs old) were used for preparing somatic chromo somes of the brain following the squash technique of Breland (1961) modified by French et al. (1962). The karyotype studies were made by employing the modified mammalian chromosome technique of Lewis and Riles (1960) and chromosome measurements were made according to the procedure used by Baker and Aslamkhan (1969). Rapid cell divisions were found to occur in the brain cells of the larvae

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Evaluation of chemosterilant damage to the testes of the housefly, Musca domestica, by microscopic observation and by measurement of the uptake of 14C-compounds

Cited by 8 publications

References 20 publications

Insect chemosterilants: a review

Insect chemosterilants: a review

The Effects of Thiotepa on the Fine Structure of the Ovarium of the Last Instar Larvae of Agrotis Ipsilon (Hufnagel) (Lepidoptera Noctuidae)

Cytogenetic Basis of Chemically-Induced Sterility in <i>Culex pipiens fatigans</i> Wiedemann

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