Evaluation of chemopreventive effects of Thymoquinone on cell surface glycoconjugates and cytokeratin expression during DMBA induced hamster buccal pouch carcinogenesis

Abstract

The present study aimed to investigate the membrane stabilizing effect of Thymoquinone (TQ) on cell surface glycoconjugates and cytokeratin expression against DMBA induced hamster buccal pouch carcinogenesis. 0.5% DMBA painting (three times per week) in hamster buccal pouches for 14 weeks resulted in the formation of well developed oral squamous cell carcinoma. We observed 100% tumor formation with marked abnormalities of glycoconjugates status in tumor bearing hamsters as compared to control animals. Oral administration of TQ at a dose of 30 mg/kg body weight, to DMBA painted hamsters on alternate days for 14 weeks, reduced the tumor formation as well as protected the levels of cell surface glycoconjugates in DMBA painted hamsters. The present study thus suggests that TQ has potent chemopreventive efficacy as well as protected the abnormalities on cell surface glycoconjugates during DMBA induced hamster buccal pouch carcinogenesis [BMB reports 2010; 43(10): 664-669]

Tinospora cordifolia extract prevents cadmium-induced oxidative stress and hepatotoxicity in experimental rats

“…Glucose, galactose, fucose, mannose, sialic acid and some acetylated derivatives of hexosamine are the main sugar moieties in glycoproteins. Being an important constituent of cell membrane, glycoconjugates plays an essential role in cell function and cell membrane functions [52]. The elevation of glycoconjugates in circulation has been reported to happen via increased turn over, secretion and/or shedding from damaged/malignant cells [53].…”

Thymoquinone as a Potential Adjuvant Therapy for Cancer Treatment: Evidence from Preclinical Studies

“…The administration of Nigella sativa seed extract enriched with TQ suppressed mice skin papillomagenesis when applied topicall (Salomi et al, 1991). In a similar fashion, 7,12-dimethylbenz[a]anthracene DMBA-induced hamster buccal squamous cell carcinoma burden was lessened when TQ was administered by gavage (Rajkamal et al, 2010). A significant attenuation has been reported in breast, gastric, and colon cancer xenografts after intra peritoneal administration of TQ (Gali-Muhtasib et al, 2008; Lei et al, 2012; Woo et al, 2013).…”

Nigella sativa and its active constituent thymoquinone in oral health

“…Although only a few studies have been performed on the effect of TQ on oral cancer, it is still promising. 60 , 63 , 64 A study by Chu et al 60 revealed that the treatment of human oral cancer with TQ induces cell death by the 2 distinct mechanisms of apoptosis and autophagy. Apoptosis is programmed cell death.…”

“… 67 , 68 Only one oral cancer prevention study through the use of TQ has been published. 63 They used 7,12 dimethyl benz (a) anthracene (DMBA) in an induced hamster buccal pouch carcinogenesis model to study TQ chemoprevention. The DMBA is known to induce multistep carcinogenesis.…”

“…For animals treated with TQ, the incidence of oral neoplasm and cytokeratin expression was significantly prevented. 63 The NSE can be used in combination with chemotherapeutics, such as cisplatin, to enhance the anticancer activity of the drug and as a protective agent for organs from its cytotoxic effect. We verified the protective effect of NSE in a DMBA-induced hamster buccal pouch carcinogenesis model treated with cisplatin.…”