Evaluation of chemopreventive activity of glutamine by the comet and the micronucleus assay in mice's peripheral blood

ABSTRACT. Previous studies in rodents treated with the pro-carcinogen 1,2-dimethylhydrazine suggested that the consumption of wheat bran protected against DNA damage in the colon and rectum. Based on this information, we evaluated wheat bran as a functional food in the prevention and treatment of colon cancer. We used the aberrant crypt focus assay to evaluate the anticarcinogenic potential of wheat bran (Triticum aestivum Dietary wheat bran chemoprevention in vivo variety CD-104), the comet assay to evaluate its antigenotoxicity potential, and the micronucleus assay to evaluate its antimutagenic potential. The wheat bran gave good antimutagenic and anticarcinogenic responses; the DNA damage decreased from 90.30 to 26.37% and from 63.35 to 28.73%, respectively. However, the wheat bran did not significantly reduce genotoxicity. Further tests will be necessary, including tests in human beings, before this functional food can be recommended as an adjunct in the prevention and treatment of colon cancer.

Section: Discussionmentioning

confidence: 99%

“…The MA used in this experiment was originally described by Hayashi et al (1990) with modifications by Oliveira et al (2009). The slides were warmed to 70°C and covered with 20 μL acridine orange in an aqueous solution (1.0 mg/mL).…”

Section: Mamentioning

confidence: 99%

Antimutagenic and anticarcinogenic effects of wheat bran in vivo

Pesarini¹,

Zaninetti²,

Mauro³

et al. 2013

“…It was performed according to Singh et al (1988) with the modifications that were reported by Oliveira et al (2009). The cells were evaluated with a fluorescence microscope (Nikon) using a 40X objective, an excitation filter of 420-490 nm, and barrier filter of 520 nm.…”

Section: Comet Assay In Peripheral Bloodmentioning

confidence: 99%

“…The treatments and protocols were conducted according to Oliveira et al (2009) with modifications, as described. In the negative control, the MEST vehicle (1% methanol, 0.1 mL/10 g bw via gavage) and cyclophosphamide vehicle (0.9% saline, 0.1 mL/10 g bw ip) were administered the first and second days of treatment.…”

Section: Animal Experimentsmentioning

confidence: 99%

Antigenotoxic and antimutagenic effects of Schinus terebinthifolius Raddi in Allium cepa and Swiss mice: A comparative study

Fedel-Miyasato¹,

Formagio²,

Auharek³

et al. 2014

ABSTRACT. It is estimated that 60% of anticancer drugs are derived directly or indirectly from medicinal plants. Schinus terebinthifolius Raddi (Anacardiaceae) is traditionally used in Brazilian medicine to treat inflammation, ulcers, and tumors. Because of the need to identify new antimutagenic agents and to determine their mechanism of action, this study evaluated the chemopreventive activity of the methanolic extract from leaves of S. terebinthifolius (MEST) in Allium cepa cells and in Swiss mice analyzing different protocols of MEST in association with DNA-damaging agents. The antigenotoxic and antimutagenic aspects in peripheral blood were evaluated using the comet and micronucleus assays, respectively. The percentage of damage reduction was used to compare the A. cepa and mice results. Our results showed for the first time that MEST can act as a chemopreventive compound that promotes cellular genome integrity by desmutagenic and bioantimutagenic activities in vegetal and animal models. This finding may therefore have therapeutic applications that can indirectly correlate to the prevention and/or treatment of the degenerative diseases such as cancer.

“…The MA was performed using the original protocol described by Hayashi et al (1990), with the modifications described by Oliveira et al (2009). The slides were warmed to 70°C and covered with a layer of 20 μL 1.0 mg/mL acridine orange in an aqueous solution.…”

Section: Mamentioning

confidence: 99%

Evaluation of the antimutagenic and anticarcinogenic effects of inulin in vivo

Mauro¹,

Monreal²,

Silva³

et al. 2013

ABSTRACT. The incidence of colorectal cancer is growing worldwide. The characterization of compounds present in the human diet that can prevent the occurrence of colorectal tumors is vital. The oligosaccharide inulin is such a compound. The aim of this study was to evaluate the antigenotoxic, antimutagenic and anticarcinogenic effects of inulin in vivo. Our study is based on 3 assays that are widely used to evaluate chemoprevention (comet assay, micronucleus assay, and aberrant crypt focus assay) and tests 4 protocols of treatment with inulin (pre-treatment, simultaneous, posttreatment, and pre + continuous). Experiments were carried out in Swiss male mice of reproductive age. In order to induce DNA damage, we used the pro-carcinogenic agent 1,2-dimethylhydrazine. Inulin was administered orally at a concentration of 50 mg/kg body weight following the protocols mentioned above. Inulin was not administered to the control groups. Our data from the micronucleus assay reveal antimutagenic effects of inulin in all protocols. The percentage of inulin-induced damage reduction ranged from 47.25 to 141.75% across protocols. These data suggest that inulin could act through desmutagenic and bio-antimutagenic mechanisms. The anticarcinogenic activity (aberrant crypt focus assay) of inulin was observed in all protocols and the percentages of damage reduction ranged from 55.78 to 87.56% across protocols. Further tests, including human trials, will be necessary before this functional food can be proven to be effective in the prevention and treatment of colon cancer.