Evaluation of Changes in Genetic Characteristics of Male Peach Fruit Fly, Bactrocera zonata Irradiated with Gamma Radiation using RAPD-PCR Technique

Zahran, N. F.; Hamza, Ali F.; Sayed, R. M.

doi:10.17582/journal.pjz/2017.49.3.951.956

Cited by 3 publications

(1 citation statement)

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“…The variations in the PCR patterns depend on the primer used, the dose of gamma irradiation given to the insect, the kind of the irradiation source and differ from the parent, F1 and F2 Rizk et al (2017) stated that the effects of γ rays on the DNA patterns of adult male parents and F1 males Galleria melonella showed alterations among the controls, the treated parents and F1 individuals. Exposure to radiation caused very frequently the appearance of some extra bands and the deficiency of others in the arbitrary random amplified polymorphic DNA-polymerase chain reaction amplification patterns of the irradiated insects also Zahran et al (2017) Full-grown male pupae of the peach or guava fruit fly, Bactrocera zonata were irradiated with sterilizing and substerilising doses of gamma radiation. The effect of gamma rays on the DNA patterns of adult males showed alterations from the control.…”

Section: Discussionmentioning

confidence: 99%

Biomarkers Changes Induced in The Mediterranean Fruit Fly, Ceratitis capitata (Wied.) Irradiated by Sterilising And Substerilising Doses of Gamma Radiation

Sayed

Abdalla

Sayed

et al. 2020

Egyptian Academic Journal of Biological Sciences. A, Entomology

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Radiation technology is widely used to produce changes in biosystems. The goal of this work is to determine the variation induced in male parents and F1 Ceratitiis capitata when gamma-irradiated as pupae using substerilising doses (50 and 70 Gy and sterilising dose 90 Gy. Biological studies showed that the percent adult emergence of irradiated pupae decreased as the dose increased and also the percent egg hatchability decreased as the dose increased, also percent pupation and percent emergence of F1 decreased as the dose increased. Comparing DNA (using RAPD-PCR) of untreated adults, adults irradiated with sterilising dose of 90 Gy and parents irradiated with substerile dose (50 and 70 Gy) and their resultant F1. The results revealed that some extra bands appeared and others disappeared, as a result of irradiation. The appearance of extra bands may be due to the repair mechanism of the irradiation damaged DNA. The banding patterns obtained and the dendrograms drawn on the basis of presence and absence of bands revealed that irradiation of parents with 50 and 70 Gy and their F1 are different from the unirradiated pupae It was concluded that the sterile male technique could be used as a benefit tool in controlling Ceratitiis capitata.

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Section: Discussionmentioning

confidence: 99%

Biomarkers Changes Induced in The Mediterranean Fruit Fly, Ceratitis capitata (Wied.) Irradiated by Sterilising And Substerilising Doses of Gamma Radiation

Sayed

Abdalla

Sayed

et al. 2020

Egyptian Academic Journal of Biological Sciences. A, Entomology

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Genetic profile of gamma irradiated Locusta migratoria migratorioides: A futuristic eco-friendly control approach

Ali

Fathy

Ibrahim

et al. 2022

Preprint

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The most common species of grasshopper in Africa is called Locusta migratoria migrotaria (L.), and it is thought to pose a severe danger to agriculture worldwide. The Locusta migratoria species developed resistance to insecticides because of overuse, which also polluted the environment. As a result, opportunities to investigate new control strategies appeared. The purpose of the current study is to assess the effectiveness of gamma radiation in eradicating Locusta species and to look into the DNA alterations caused by radiation exposure in both male and female insects. Males and female adults (around one-month-old) received radiation treatments of 10, 20, 30, and 40 Gy. DNA isolation and Start codon targeted polymorphism (SCoT-PCR) analysis were done, along with mortality percentage calculations. The death rate increased significantly over time as a result of gamma irradiations resulting in an estimated LD50 value for males and females of 33.94 and 51.55Gy, respectively. According to the SCoT research, the adults' radiation exposure resulted in the disappearance of some bands and the appearance of new additional bands. The similarity index was used to create a dendrogram. It was determined that irradiating the pest may be used as a technique to kill the insect and stop its spread. In addition, it resulted in certain genetic alterations within this species. Conclusions: The control of L. may be suggested by utilizing radiation technology, after further field studies.

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Genetic Variation in Peach Fruit Fly, Bactrocera zonata, Females Irradiated with Gamma Radiation as Revealed by RAPD-PCR Technique

Sayed¹,

Zahran²,

Hamza

2022

Entomological News

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Evaluation of Changes in Genetic Characteristics of Male Peach Fruit Fly, Bactrocera zonata Irradiated with Gamma Radiation using RAPD-PCR Technique

Abstract: (NCRRT) for her critical comments, proceeding facilities, offering help in revising the manuscript, valuable support and kind help.

Cited by 3 publications

References 11 publications

Biomarkers Changes Induced in The Mediterranean Fruit Fly, Ceratitis capitata (Wied.) Irradiated by Sterilising And Substerilising Doses of Gamma Radiation

Biomarkers Changes Induced in The Mediterranean Fruit Fly, Ceratitis capitata (Wied.) Irradiated by Sterilising And Substerilising Doses of Gamma Radiation

Genetic profile of gamma irradiated Locusta migratoria migratorioides: A futuristic eco-friendly control approach

Genetic Variation in Peach Fruit Fly, Bactrocera zonata, Females Irradiated with Gamma Radiation as Revealed by RAPD-PCR Technique

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