Evaluation of Cerebral Blood Flow Autoregulation in the Rat Using Laser Doppler Flowmetry

Allen, Linda A; Terashvili, Maia; Gifford, Alison; Lombard, Julian H.

doi:10.3791/60540

Cited by 2 publications

(3 citation statements)

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“…Одним из убедительных аспектов ЛДФ является его способность диагносцировать артериальную или венозную окклюзию. Существует корреляция выживаемости лоскутных трансплантатов со снижением лазерного допплеровского сигнала [14,15,16,17].…”

Section: лазерная допплеровская флоуметрияunclassified

“…В частности, в неврологии прижизненная диагностика используется в многочисленных исследованиях изучения динамики роста нейронов. В данном обзоре литературы были перечислены основные на сегодняшний день методы, которые позволяют более детально провести оценку МЦ в эксперименте в том числе [2,17,27,35,46]. Тем не менее, прижизненные методы мониторинга МЦ, даже на сегодняшний день, сложный и затратный финансово метод.…”

Section: заключениеunclassified

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Methods for experimental monitoring of tissue vascularization

Guseynov

Ивашкевич

Bopkhoev

et al. 2023

Medicinskij alfavit

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Microcirculation plays a decisive role in the metabolism processes between blood and tissue in both physiological and pathological conditions of the body. The analysis of changes in microcirculation provides a unique perspective for studying processes at the micro- and macrolevels in clinical and experimental medicine, including diseases in the maxillofacial region. Despite the critical role of microcirculation in many oral diseases, direct imaging and quantification of tissue microcirculation is currently limited. Scientific interest in microcirculatory monitoring is growing with the advent of new physical diagnostic methods. To date, there are many methods for assessing microcirculation. The purpose of this literature review is to show the positive and negative aspects of using various methods for monitoring microcirculation. The scientific literature was searched in the PubMed and Elibrary databases. As a result, the main shortcomings of various monitoring methods were emphasized, as well as what prospects for study and modification are possible today.

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Section: лазерная допплеровская флоуметрияunclassified

Section: заключениеunclassified

Methods for experimental monitoring of tissue vascularization

Guseynov

Ивашкевич

Bopkhoev

et al. 2023

Medicinskij alfavit

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“…The broadening of the laser light, dependent on the velocity of the moving erythrocytes, is subsequently detected by photodetectors and processed by a computer. To date, LDF probes have been applied to various vascularized tissue beds (e.g., skin, muscle, liver, brain, bone, and tooth pulp) in both experiments in humans ( Davis et al, 2006 ; Davis et al, 2008 ; Ghouth et al, 2018 ) and animals ( Arvidsson et al, 1988 ; Lombard and Roman, 1990 ; Hanne et al, 2019 ; Allen et al, 2020 ). The exact sampling depth of an LDF probe is difficult to determine.…”

Section: Introductionmentioning

confidence: 99%

Multiple Laser Doppler Flowmetry Probes Increase the Reproducibility of Skin Blood Flow Measurements

et al. 2022

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Cutaneous microcirculatory perfusion is commonly measured using laser Doppler flowmetry (LDF) probes, which provide a continuous, non-invasive quantification of skin blood flow (SkBF). However, inhomogeneities in the skin’s microvasculature density contribute to a decrease in reproducibility whenever an LDF probe is removed and replaced, as is the case during pre- and post-intervention or between-day measurements. Therefore, this study aimed to determine whether increasing the total number of individual LDF probes in a localized area improves the reproducibility of the measurement. Seven laser Doppler probes were secured in a custom-made acrylic holder designed to attach to the skin’s surface easily. SkBF, local skin temperature (Tsk), and blood pressure (BP) were assessed in 11 participants (6 M, 5 F, 42 ± 15 years). SkBF and Tsk were measured from the dorsal forearm (arm trial) for 5 min. Next, the multi-laser device was moved to the lateral side of the calf (leg trial), and measurements were obtained for 5 min. Each arm and leg trial was cyclically repeated three times, and all trials were separated by intermissions lasting 10–15 min. The average SkBF and the cutaneous vascular conductance (CVC) from all possible LDF probe combinations were not statistically different across the three arm and leg trials. Two-way mixed-effects models with absolute agreement were used to compute the intraclass correlation coefficient (ICC) for CVC, and the minimum ICC increased with the addition of LDF probes. The ICC of the average CVC from seven LDF probes was 0.96 between the arm trials and 0.91 between the leg trials, which suggests that there is excellent reliability and little difference between trials following the removal and replacement of the device. Moreover, all individual ICC values from ≥3 LDF probe combinations were greater than 0.70 (i.e., good reliability). These data suggest that SkBF measurements with multiple laser Doppler probes in a custom-made holder have excellent reproducibility after replacing the probes within the same participant. Therefore, this application could provide more reproducible assessments between repeated measurements (e.g., before and after exercise or clinical procedures) where the LDF probes must be removed and replaced within the same location.

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Evaluation of Cerebral Blood Flow Autoregulation in the Rat Using Laser Doppler Flowmetry

Cited by 2 publications

References 0 publications

Methods for experimental monitoring of tissue vascularization

Methods for experimental monitoring of tissue vascularization

Multiple Laser Doppler Flowmetry Probes Increase the Reproducibility of Skin Blood Flow Measurements

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