Evaluation of central and peripheral effects of doxepin on acetic acid-induced colitis in rat and the involved mechanisms

Zabihi, Mohsen; Hajhashemi,; Talebi, Ardeshir; Minaiyan, Mohsen

doi:10.17179/excli2016-727

Cited by 3 publications

(1 citation statement)

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“…This may again be due to the relatively short application times of the irritant, maybe too short for these cells to activate. Other authors [ 22 , 27 , 43 , 44 ] detected an increase in MPO expression after performing intracolonic treatments with 4% acetic acid. However, measurements for MPO detection were made several hours or even days after treatment, in contrast with our early protocol.…”

Section: Discussionmentioning

confidence: 92%

Effects of Commercial Probiotics on Colonic Sensitivity after Acute Mucosal Irritation

Gómez

Antón

López-Tofiño

et al. 2022

IJERPH

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Gastrointestinal pathologies associated with abdominal pain, such as irritable bowel syndrome or inflammatory bowel disease, lack sufficiently effective treatments. In our study we have used a rat model of visceral pain (72 animals; n = 8–13 per experimental group) to analyze the consequences of intracolonic administration of the irritant acetic acid on visceral sensitivity, histology of the colonic wall, and inflammatory response. Moreover, we have studied the possible beneficial effects of a pretreatment with a commercial probiotic (Actimel®). Contrary to expectations, acetic acid application (7 cm proximal to the anus) decreased the nociceptive response to intracolonic mechanical stimulation, with a slight increase in the histological damage of colonic mucosa. The intensity of these changes depended on the concentration (4% or 0.6%) and the time of application (30 or 60 min). Pretreatment with probiotics (by daily gavage, for 1 week) normalized the values obtained in the visceral sensitivity test but revealed an increase in the number of macrophages. These results suggest a possible activation of inhibitory mechanisms early after colonic irritation, not previously described (which need further experimental confirmation), and the ability of probiotics to normalize the effects of acetic acid. In addition, pretreatment with probiotics has a direct effect on immune functions, stimulating macrophagic activity.

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