Evaluation of CD30 as a marker for Th2 lymphocytes in bronchoalveolar lavage in interstitial lung diseases

Petkova, D.; Xaubet, Antoni; Picado, César; Filella, X; Agustı́, Carlos; Luburich, Patricio; Rodríguez-Roisin, Roberto

doi:10.1053/rmed.1999.0800

Cited by 8 publications

(2 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To date, a wide variety of cells and cell lines have been shown to express CD30. These include activated T cells, predominantly Th2 type [16,17,18], T cytotoxic (Tc)2 type [19], CD8 [20], gamma/delta [21], NK cells [22], and lymphocytes infected with HIV [23] or EBV [24]. Although numerous studies have attempted to elucidate the physiological and pathological roles of CD30, the actual roles of the CD30 molecules in vivo remain obscure [25].…”

Section: Introductionmentioning

confidence: 99%

Rapid and Strong Induction of Apoptosis in Human Eosinophils by Anti-CD30 mAb-Coated Microspheres and Phagocytosis by Macrophages

Matsumoto

Terakawa²,

Fukuda³

et al. 2007

Int Arch Allergy Immunol

View full text Add to dashboard Cite

Background: Eosinophils represent a potential therapeutic target in allergic diseases. We previously reported that two clones of anti-CD30 mAbs (HRS-4 and Ber-H8) induced extremely rapid and intense apoptosis in human eosinophils in vitro, but only when the mAbs were immobilized on plates [Matsumoto K, J Immunol 2004;172:2186]. As the initial step towards clinical application of these anti-CD30 mAbs in the treatment of allergic diseases, we made an attempt to clarify two issues; first, whether or not anti-CD30 mAb-coated microspheres can efficiently induce apoptosis in human eosinophils, and second, whether or not these apoptotic eosinophils can be phagocytosed by macrophages without the release of granular proteins. Methods: Purified human eosinophils were treated with anti-CD30 mAb-coated polystyrene microspheres (diameter, 1.44 µm). Apoptosis was determined by annexin V-binding. For the phagocytosis assay, eosinophils were co-cultured with monocyte-derived human macrophages or PMA-pretreated U-937 cells. Phagocytosis was determined by light microscopy and by the eosinophil-derived neurotoxin (EDN) concentration in the supernatant. Results: Anti-CD30 mAb-coated, but not control IgG1-coated microspheres significantly reduced eosinophil survival in a dose-dependent manner. Marked phagocytosis of the apoptotic eosinophils by macrophages was also observed when the eosinophils were treated with anti-CD30 mAb-coated microspheres. The apoptotic eosinophils released large amounts of EDN in the absence of macrophages; however, the EDN levels were significantly decreased when the eosinophils were co-cultured with macrophages. Conclusions: Anti-CD30 mAb-coated microspheres are capable of inducing rapid and strong apoptosis in human eosinophils. Furthermore, the apoptotic eosinophils were also phagocytosed by macrophages with minimal release of the granular proteins.

show abstract

Section: Introductionmentioning

confidence: 99%

Rapid and Strong Induction of Apoptosis in Human Eosinophils by Anti-CD30 mAb-Coated Microspheres and Phagocytosis by Macrophages

Matsumoto

Terakawa²,

Fukuda³

et al. 2007

Int Arch Allergy Immunol

View full text Add to dashboard Cite

show abstract

“…The other T-helper subtype, Th1, producing cytokines such as IL-2, IL-12, and IFN-g, is involved in immune responses against intracellular pathogens such as viruses and tends to antagonise the allergic response. Recently the CC-chemokine receptor 5 (CCR5) has been described as a surface marker of Th1 cells (Odum et al, 1999), whereas the relationship between CD30 expression and Th2 cells is still controversial (Hamann et al, 1996;Petkova et al, 2000).…”

Section: Th1 -Th2 Regulation and Allergymentioning

confidence: 99%

Mathematical Modelling of Venom Immunotherapy

Richter

Metzner²,

Behn³

2002

Computational and Mathematical Methods in Medicine

View full text Add to dashboard Cite

Allergic hypersensitivity of type I for hymenoptera venoms is the most frequent reason for acute IgEmediated anaphylactic reactions. The subcutaneous injection of increasing doses of purified allergen followed by long-term administration of an adequate maintenance dose over a period of 3-5 years called venom immunotherapy (VIT) has been proven to be a very effective treatment achieving protection in about 96% of allergic patients. Even though the principle of hyposensitisation has been introduced already 90 years ago, the underlying immunoregulatory mechanisms of VIT remain poorly understood. Recent studies suggest a shift in cytokine production from a Th2 to a Th1 cytokine profile during therapy. In this paper, a mathematical model for T-cell regulation and a model for mast cell/basophil desensitisation are presented and analysed to explain the mechanism of conventional rush and ultra-rush VIT.

show abstract

Bronchoalveolar lavage improves diagnostic accuracy in patients with diffuse lung disease

Capelozzi

Faludi

Balthazar

et al. 2011

Diagnostic Cytopathology

View full text Add to dashboard Cite

Bronchoalveolar lavage (BAL) is an established diagnostic tool in diffuse parenchyma lung disease. The objective of the present study was designed to investigate whether immunophenotyping affects BAL results and improves diagnostic accuracy. BAL from 61 patients was included in the study. The patients were also submitted to transbronchial biopsy, with a final diagnosis of granulomatous disease [tuberculosis (TB), n = 20; sarcoidosis (SARC), n = 3; and hypersensitivity pneumonitis (HP), n = 4]; idiopathic interstitial pneumonias (IIPs) [idiopathic pulmonary fibrosis (IPF), n = 9; organizing pneumonia (OP), n = 17]; and lung cancer (LC), n = 8. Immunohistochemistry and histomorphometry were used to identify and quantify type 1 and type 2 alveolar epithelial cells, macrophages, CD3+T-cells, CD4+T-cells, CD8+T-cells, and CD20+B-cells in BAL. These markers were correlated with a database and pulmonary function tests. The cellular, inflammatory, and immune components of BAL varied among the diagnostic groups and were negatively correlated with age and smoking history. An increased quantity of lymphocyte surface markers CD3 (P < 0.05) and CD20 (P = 0.01) was seen in IIPs. Patients with a pattern of OP had a higher proportion of type 2 alveolar epithelial cells; patients with SARC had a higher density of CD20+B-cells and CD4+T-helper cells; and patients with HP had a higher proportion of CD8+T-cytotoxic cells. A positive association was found between the density of type I alveolar epithelial cells and forced vital capacity. The immunophenotyping affects the cellular, inflammatory, or immune constituents of BAL and improved the diagnostic accuracy in diffuse parenchymal lung disease.

show abstract

Evaluation of CD30 as a marker for Th2 lymphocytes in bronchoalveolar lavage in interstitial lung diseases

Cited by 8 publications

References 24 publications

Rapid and Strong Induction of Apoptosis in Human Eosinophils by Anti-CD30 mAb-Coated Microspheres and Phagocytosis by Macrophages

Rapid and Strong Induction of Apoptosis in Human Eosinophils by Anti-CD30 mAb-Coated Microspheres and Phagocytosis by Macrophages

Mathematical Modelling of Venom Immunotherapy

Bronchoalveolar lavage improves diagnostic accuracy in patients with diffuse lung disease

Contact Info

Product

Resources

About