Evaluation of capillary electrophoresis for the analysis of nicotine and selected minor alkaloids from tobacco

Yang, Shenyuan; Smetena, I.

doi:10.1007/bf02269897

Cited by 65 publications

(42 citation statements)

References 10 publications

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“…At high pH the EOF rate is highest, but in free base form, nicotine and related alkaloids are carried by the EOF to the detector unresolved [1]. At low pH, the cationic character of the analytes is revealed, they separate and migrate towards the detector without the presence of an EOF.…”

Section: Free-solution Cementioning

confidence: 97%

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Orthogonal separations of nicotine and nicotine‐related alkaloids by various capillary electrophoretic modes

2004

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The migration behaviour of nicotine and related tobacco alkaloids was investigated using three different capillary electrophoretic (CE) modes. Novel separations were achieved both using microemulsion electrokinetic chromatography (MEEKC) and nonaqueous CE (NACE). Improved resolution compared to previous studies was obtained using free-solution CE (FSCE). Each technique resulted in different, orthogonal separation selectivity. The suitability of each method for application to the analysis of nicotine lozenges is discussed. The FSCE method was applied to the analysis of nicotine lozenges due to its compatibility with an established lozenge extraction solvent. The method used gave good injection precision and linearity. Good agreement of CE and high-performance liquid chromatography (HPLC) results was obtained. The CE method is therefore considered suitable for the quantitative determination of nicotine in nicotine lozenges.

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Section: Free-solution Cementioning

confidence: 97%

“…Figure 1 shows the structures of nicotine and major degradants. In aqueous solution, nicotine exists as a free base or in its mono-protonated (pKa 1 8.02) or di-protonated (pKa 2 3.12) forms, depending on the pH of the solution [1]. During analysis, this pH-dependent character must be carefully controlled.…”

Section: General Aspectsmentioning

confidence: 99%

Orthogonal separations of nicotine and nicotine‐related alkaloids by various capillary electrophoretic modes

2004

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“…Other analysis approaches have included high-performance liquid chromatography-ultraviolet detection (HPLC-UV), capillary zone electrophoresis-ultraviolet detection (CZE-UV), micellar electrokinetic capillary chromatography-ultraviolet detection (MECC-UV), nitrogen chemiluminescence detection (NCD), and microemulsion electrokinetic chromatographyultraviolet detection (MEEKC-UV). [10][11][12][13][14][15][16][17] While more extravagant and/or complex approaches can be utilized, each exhibit certain limitations (i.e. standard addition construction, elaborate detection methods).…”

Section: Introductionmentioning

confidence: 99%

Application of GC-MS/MS for the Analysis of Tobacco Alkaloids in Cigarette Filler and Various Tobacco Species

et al. 2013

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This publication reports the first known use of gas chromatography-tandem mass spectrometry for the quantitation of five minor tobacco alkaloids (nornicotine, myosmine, anabasine, anatabine and isonicoteine) in various tobacco samples. A summary of the concentrations of these minor alkaloid levels in the filler from 50 popular cigarette brands were found to be 659 -986 μg/g nornicotine, 8.64 -17.3 μg/g myosmine, 127 -185 μg/g anabasine, 927 -1390 μg/g anatabine, and 23.4 -45.5 μg/g isonicoteine. Levels of minor alkaloids found in reference cigarettes (1R5F, 2R4F, 3R4F, CM4 and CM6) as well as burley, flue-cured, oriental, reconstituted, Nicotiana rustica and Nicotiana glauca tobacco types are also reported. Quantitation of the minor tobacco alkaloids is important because the alkaloids have been shown to be precursors of carcinogenic tobacco specific N′-nitrosamines.

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“…Recently, several studies dealing with herbal medicines, have been reported and two kinds of medicinal compounds, i.e. alkaloids [95][96][97][98][99][100][101][102] and flavonoids [103][104][105][106][107][108], have been studied extensively.…”

Section: Electrophoretic Methodsmentioning

confidence: 99%

Quality control of herbal medicines

Liang

Xie²,

Chan

2004

Journal of Chromatography B

324

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Different chromatographic and electrophoretic techniques commonly used in the instrumental inspection of herbal medicines (HM) are first comprehensively reviewed. Chemical fingerprints obtained by chromatographic and electrophoretic techniques, especially by hyphenated chromatographies, are strongly recommended for the purpose of quality control of herbal medicines, since they might represent appropriately the "chemical integrities" of the herbal medicines and therefore be used for authentication and identification of the herbal products. Based on the conception of phytoequivalence, the chromatographic fingerprints of herbal medicines could be utilized for addressing the problem of quality control of herbal medicines. Several novel chemometric methods for evaluating the fingerprints of herbal products, such as the method based on information theory, similarity estimation, chemical pattern recognition, spectral correlative chromatogram (SCC), multivariate resolution, etc. are discussed in detail with examples, which showed that the combination of chromatographic fingerprints of herbal medicines and the chemometric evaluation might be a powerful tool for quality control of herbal products.

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Evaluation of capillary electrophoresis for the analysis of nicotine and selected minor alkaloids from tobacco

Cited by 65 publications

References 10 publications

Orthogonal separations of nicotine and nicotine‐related alkaloids by various capillary electrophoretic modes

Orthogonal separations of nicotine and nicotine‐related alkaloids by various capillary electrophoretic modes

Application of GC-MS/MS for the Analysis of Tobacco Alkaloids in Cigarette Filler and Various Tobacco Species

Quality control of herbal medicines

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