Application of GC-MS/MS for the Analysis of Tobacco Alkaloids in Cigarette Filler and Various Tobacco Species

Lisko, Joseph G.; Stanfill, Stephen B.; Duncan, Bryce W.; Watson, Clifford H.

doi:10.1021/ac400077e

Cited by 77 publications

(81 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Observed differences in alkaloid content are most likely due to the variations in cigarette filler composition. Overall, the results on the determination of nicotine and three minor alkaloids obtained in this study compare well within ranges reported for similar products in other studies [7,31,32]. Proposed HILIC-MS/MS technique offers distinct advantages over previous methods, including unambiguous analyte identification, ease of sample preparation, enhanced sensitivity, and high throughput.…”

Section: Analytical Performancesupporting

confidence: 86%

Determination of nicotine and three minor alkaloids in tobacco by hydrophilic interaction chromatography-tandem mass spectrometry

Taujenis¹,

Olšauskaitė²,

Padarauskas³

2015

Acta Chromatographica

View full text Add to dashboard Cite

Summary.A reliable, sensitive and rapid method for determination of nicotine and three minor alkaloids (cotinine, anabasine and nornicotine) in tobacco by ultra-high performance liquid chromatography in hydrophilic interaction chromatography mode coupled with tandem mass spectrometry (HILIC-MS/MS) has been established. HILIC separation was performed on a BEH HILIC column using isocratic elution at 0.5 mL/min with acetonitrile:water (85:15, v/v) mobile phase containing 5 mmol/L ammonium acetate (pH 5.00). Separated analytes were determined by electrospray ionization MS/MS in the positive ion mode using multiple reaction monitoring. Alkaloids from tobacco were extracted in an ultrasonic bath for 10 min with acetonitrile:water mixture (8:2, v/v) containing 5 mmol/L ammonium acetate (pH 5.00). Limits of quantification were 10 µg/g for cotinine, 20 µg/g for anabasine and nornicotine, and 30 µg/g for nicotine. Mean recoveries from tobacco ranged between 94.8% and 104.1% for different analytes with relative standard deviations within 5%. The performance of the proposed method was tested for the extraction and determination of the four alkaloids in cigarette tobacco fillers, and satisfactory results were achieved.

show abstract

Section: Analytical Performancesupporting

confidence: 86%

Determination of nicotine and three minor alkaloids in tobacco by hydrophilic interaction chromatography-tandem mass spectrometry

Taujenis¹,

Olšauskaitė²,

Padarauskas³

2015

Acta Chromatographica

View full text Add to dashboard Cite

show abstract

“…It grows well in warm-arid and semiarid climates on marginal unfertile lands (Biblioteca digital de la medicina tradicional Mexicana, 2009). High levels of anabasine, a piperidine alkaloid isomer of nicotine, have been reported in this plant as far as 1935 (Smith, 1935;Galiana and VigueraLoko, 1964), and confirmed in many other chemical studies (Lisko et al, 2013). Ingestion of this alkaloid can result in severe or lethal poisoning in humans, livestock and poultries (Papavisiliu and Heliakis, 1947;Castorena et al, 1987;Sims et al, 1999;Mizrachi et al, 2000;Schep et al, 2009;Botha et al, 2011;Semmler et al, 2012).…”

Section: Introductionmentioning

confidence: 70%

Anti-Inflammatory and Antioxidant Activities of Methanol Extracts and Alkaloid Fractions of four Mexican Medicinal Plants of Solanaceae

et al. 2014

Afr. J. Trad. Compl. Alt. Med.

View full text Add to dashboard Cite

Background: Methanol extracts and alkaloid fractions of different parts of four plant species belonging to Solanaceae family and used in Mexican traditional medicine were investigated for their total phenolic contents, anti-inflammatory and antioxidant properties. Materials and Methods: The total phenolic compounds of each extract was determined according to the Folin-Ciocalteu method, while the in vitro radical scavenging activities of the extracts were assessed using the DPPH and ABTS radicals. The in vivo anti-inflammatory activity was determined using the TPA-induced mouse ear edema model. Results:The methanol extracts contained the highest concentrations of phenolic compounds and also exhibited the best reducing power on the DPPH and ABTS radicals, in a concentration-dependent fashion. However, the anti-inflammatory activity did not follow the same trend, as some alkaloid fractions that showed low radical reducing power exhibited the strongest anti-inflammatory activity. Conclusion:The methanol extract obtained from the flowers of Nicotiana glauca presented the best overall performance with the largest amount of phenolic compounds (111 µg garlic acid equivalents/g of extract), the best antioxidant activity (94.80% inhibition of DPPH and 97.57% of ABTS) and the highest anti-inflammatory activity (81.93% inhibition of the inflammation).

show abstract

“…These impurities and degradation products (structures shown in Figure 1) can be transferred to the aerosol and subsequently to the user of the device. There are many methods for the analysis of nicotine and related alkaloids including gas chromatography coupled with flame ionization detection (FID) (6, 7), nitrogenphosphorus detection (NPD) (8), and mass spectrometry (MS) (9). However, methods for the analysis of nicotine in tobacco smoke and products may not be suitable for e-liquids and aerosols since several of the nicotine related compounds such as nicotine-N-oxide, are known to be thermally unstable at temperatures required for GC analysis.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Nicotine and Nicotine-Related Compounds in Electronic Cigarette Liquids and Aerosols by Liquid Chromatography-Tandem Mass Spectrometry

Liu¹,

Joza²,

Rickert³

2017

Beiträge Zur Tabakforschung International/Contributions to Tobacco Research

View full text Add to dashboard Cite

SUMMARYThe objective of this study was to develop and validate an analytical method for determining nicotine and nicotine related compounds (i.e., nicotine-N-oxide, cotinine, nornicotine, anatabine, myosmine, anabasine, and β-nicotyrine) in e-cigarette aerosols and e-liquids. Aerosol collection was achieved using a Cambridge collection pad. The sample preparation consisted of adding deuterated internal standards to the collection pad and extracting with 100 mM ammonium acetate solution using a wrist-action shaker. The filtrate was then analyzed by LC-MS/MS using a Gemini NX C 18 column (3 µm, 150 × 3 mm) with a mobile phase gradient system consisting of acetonitrile and 10% acetonitrile in 10 mM ammonium bicarbonate (pH = 8.0) and electrospray ionization (ESI) in the positive mode. The e-liquid was analyzed using the same instrumental parameters, but simplifying the sample preparation procedure by adding deuterated internal standards directly to the 100-mg sample. The sample was then extracted with 100 mM ammonium acetate solution, sonicated, and filtered. In this study, the method's accuracy, robustness, and reliability were enhanced by using deuterated analogues of each compound as internal standards and by applying two ion-transition pairs for each compound for the confirmation and quantification. Validation experiments demonstrated good sensitivity, specificity and reproducibility. All the target compound calibrations exhibited satisfactory linearity from 0.050 to 5.0 mg/mL (r 2 > 0.995). The average recoveries for e-liquids varied from 85.2%(nicotine-N-oxide) to 110% (β-nicotyrine) with recoveries for all compounds exhibiting a coefficient of variation (CV) < 5.0%. Similarly, the average recoveries for e-cigarette aerosols varied from 87.8% (for nicotine-N-oxide) to 111% (for myosmine) with all CV < 8.8%. The LOD and LOQ for e-liquids for all target compounds ranged from 0.234 and 0.781 μg/g (cotinine) to 1.66 and 5.48 μg/g (nicotine-Noxide). For e-cigarette aerosols these limits ranged from 0.094 and 0.312 μg/collection (cotinine) to 0.872 and 2.87 μg/collection (nicotine-N-oxide). This methodology was used to quantitatively determine if any of the target compounds were present in a variety of sample matrices, including e-cigarette solutions and aerosols, and was successfully applied to stability studies, to monitor changes in the target compound levels which might be caused by e-cigarette formulations, components and the storage conditions. [Beitr. Tabakforsch. Int. 27 (2017)

show abstract

Application of GC-MS/MS for the Analysis of Tobacco Alkaloids in Cigarette Filler and Various Tobacco Species

Cited by 77 publications

References 19 publications

Determination of nicotine and three minor alkaloids in tobacco by hydrophilic interaction chromatography-tandem mass spectrometry

Determination of nicotine and three minor alkaloids in tobacco by hydrophilic interaction chromatography-tandem mass spectrometry

Anti-Inflammatory and Antioxidant Activities of Methanol Extracts and Alkaloid Fractions of four Mexican Medicinal Plants of Solanaceae

Analysis of Nicotine and Nicotine-Related Compounds in Electronic Cigarette Liquids and Aerosols by Liquid Chromatography-Tandem Mass Spectrometry

Contact Info

Product

Resources

About