Evaluation of calcium dobesilate for its anti-cataract potential in
experimental animal models

Velpandian, Thirumurthy; Nirmal, J.I. Kumar; Gupta, Pankaj; Vijayakumar, A R; Ghose, S

doi:10.1358/mf.2010.32.3.1423888

Cited by 9 publications

(7 citation statements)

References 35 publications

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“…The procedure previously standardized in our laboratory [6] was used for this experiment. Briefly, ten days old White leghorn chick eggs were procured from Kegg Farm (Gurgaon, Haryana, India).…”

Section: Methodsmentioning

confidence: 99%

Evaluation of pharmacological activities and assessment of intraocular penetration of an ayurvedic polyherbal eye drop (Itone™) in experimental models

Velpandian

Gupta

Ravi

et al. 2013

BMC Complement Altern Med

220

154

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BackgroundThe polyherbal eye drop (Itone™) is a mixture of aqueous distillates of nineteen traditionally used ingredients that sum up to impart potency to the formulation and make it a useful adjunct in various ocular pathologies. However, as there have been no controlled experimental studies accounting to the above claim, therefore, the present study was designed to evaluate the polyherbal formulation (PHF) for antiangiogenic, anti-inflammatory, anticataract, antioxidant and cytotoxicity in addition to the evaluation of intraocular penetration of PHF in rabbit eyes using LC-MS/MS.Materials and methodsAntiangiogenic activity of the PHF was evaluated using in ovo chick chorio-allantoic membrane (CAM) assay and in vivo cautery induced corneal neovascularization assay in rats. Anticataract potential was evaluated using steroid induced cataract in developing chick embryos, sodium selenite induced cataract in rat pups and galactose induced cataract in rats. The antioxidant activity was evaluated using di-phenyl picryl hydrazyl (DPPH) radical scavenging assay. Anti-inflammatory activity was evaluated in vitro using inhibition of LTB4 formation in human WBCs and in vivo using carrageenan induced paw edema assay in rats. The cytotoxicity was evaluated against HeLa cancer cell lines using (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Furthermore evaluation of the intraocular penetration of the PHF was carried out in rabbit eyes via aqueous humor paracentesis and further analysis using LC-MS/MS.ResultsPHF significantly inhibited VEGF induced proliferation of new blood vessels in CAM assay and inhibited the cautery induced corneal neovascularization in rats. Additionally, PHF showed noticeable delay in the progression of cataract in the selenite and galactose induced cataract models whereby the PHF treated lenses were graded for stages II and III respectively. However, the PHF did not show any anticataract activity in the hydrocortisone induced cataract model. Moreover, PHF exhibited anti-inflammatory activity whereby it showed 39.34% inhibition of LTB4 formation and significantly inhibited carrageenan induced paw edema in rats. Eight compounds of PHF viz. camphor, casticin, curcumin-II, quercetin, rosmarinic acid, γ-terpinene, β-pinene and dipentene exhibited transcorneal penetration in rabbit eyes.ConclusionThe significant antiangiogenic and anti-inflammatory activities evinced by the PHF merits further investigation for ocular neovascular and inflammatory diseases in humans.

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Section: Methodsmentioning

confidence: 99%

Evaluation of pharmacological activities and assessment of intraocular penetration of an ayurvedic polyherbal eye drop (Itone™) in experimental models

Velpandian

Gupta

Ravi

et al. 2013

BMC Complement Altern Med

220

154

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“…Calcium is closely related to vitamin D, and studies on experimental animal models have reported that calcium could show anti-cataract activity in steroid-mediated cataracts with its antioxidant effect [27]. Paradoxically, it has also been stated that calcium could start cataract development by inducing cortical opacities following protease induction in lens epithelial cells [28, 29].…”

Section: Discussionmentioning

confidence: 99%

Vitamin D Levels in Young Adult Cataract Patients: A Case-Control Study

Öktem

Aslan²

2020

Ophthalmic Res

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Objective: We aimed to investigate whether a relationship is present between early cataract formation and vitamin D in young adults. Methods: A total of 37 cataract patients (18 males and 19 females) and 53 healthy participants (27 males and 26 females) under the age of 60 years were included in this study. The 25-OH vitamin D values were measured in all subjects and the mean vitamin D levels compared between the 2 groups. Additionally, the differences between the vitamin D levels of the genders in both groups were investigated. Results: The mean age of the study group was 48.1 ± 8.5 (range 33–59) years, and the mean age of the control group was 49.3 ± 7.8 (range 31–59) years (p = 0.48 and p = 0.83). The mean vitamin D level was 15.6 ± 8.4 ng/mL in the study group and 20.8 ± 7.1 ng/mL in the healthy subjects (p = 0.002). Among the females, the vitamin D level was 10.6 ± 4.7 ng/mL in the study group and 18.1 ± 6.4 ng/mL in the control group (p = 0.0001). No significant difference was found between the groups among the males (p = 0.24). Conclusion: We found vitamin D deficiency to be associated with early age-related cataract in a statistically significant manner. We believe it is worth investigating the reason for this concurrence with large longitudinal studies.

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“…The present study was aimed to examine the preventive effects of different doses of betaine on cataract formation using in vivo chick embryo SIC model. Chick embryo SIC model has been used as an efficient animal cataract model to study the anti-cataract potential of several agents1617181920.…”

Section: Discussionmentioning

confidence: 99%

“…In this study, chick embryo SIC model was used which was first reported by Lee et al 15. Several studies have shown that the anatomy and histology of the chick embryo lens tissues are similar in size and appearance to human lens tissues151617.…”

Section: Methodsmentioning

confidence: 99%

Anticataractogenic effect of betaine in chick embryo hydrocortisone-induced cataract model

et al. 2019

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Background & objectives:Cataract is one of the leading causes of blindness in the world. The aim of the present study was to investigate anticataractogenic effect of betaine in chick embryo hydrocortisone (HC)-induced cataract model.Methods:The study included 60 fertilized eggs divided into six groups each having 10 eggs: one group treated with only HC (HC group); three treated with both HC and different doses of betaine (HC/B 1.00, HC/B 0.50 and HC/B 0.25 groups) and two non-HC groups treated with only phosphate-buffered saline (PBS group) or betaine (B group). After the injections, lenses of the embryos were removed and classified into five stages according to the lens opacification. The amounts of reduced glutathione (GSH) in the removed lenses were measured.Results:All the lenses in non-HC-treated groups were clear, whereas in the HC-treated group, 90 per cent of the lenses had cataract (stages 4 and 5). The mean score of lens opacity was significantly lower in all HC/B groups compared to HC group (2.4-3.5 vs. 4.4, P<0.05). Among HC/B groups, the HC/B 0.25 group had significantly lower mean score of lens opacity compared to remaining HC/B groups treated with higher doses of betaine. In addition, the mean reduced GSH level was significantly higher in HC/B 0.25 group compared to HC, HC/B 1.00 and HC/B 0.50 groups (P<0.001).Interpretation & conclusions:The present results show beneficial anti-cataract and anti-oxidant effects of 0.25 μmol/egg betaine on HC-induced cataract in the chick embryo.

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Evaluation of calcium dobesilate for its anti-cataract potential in experimental animal models

Cited by 9 publications

References 35 publications

Evaluation of pharmacological activities and assessment of intraocular penetration of an ayurvedic polyherbal eye drop (Itone™) in experimental models

Evaluation of pharmacological activities and assessment of intraocular penetration of an ayurvedic polyherbal eye drop (Itone™) in experimental models

Vitamin D Levels in Young Adult Cataract Patients: A Case-Control Study

Anticataractogenic effect of betaine in chick embryo hydrocortisone-induced cataract model

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