EVALUATION OF Ca1 ANTIBODY IN PLEURAL BIOPSY MATERIAL

Burnett, R A; Deery, Alastair R.S.; Adamson, Michael; Liddle, C N; Thomas, M.; Roberts, G. Hefin

doi:10.1016/s0140-6736(83)92884-2

Cited by 9 publications

(2 citation statements)

References 2 publications

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“…A previous immunohistological study of pleural biopsy specimens detected Cal in four of eight cases of benign mesothelium, seven of 12 cases of mesothelioma, and three of five cases of metastatic car- 22 cinoma. Our findings are consistent with these observations and iidicate that these antibodies do not distinguish between malignant and benign mesothelium in histological sections.…”

Section: Discussionmentioning

confidence: 80%

Immunohistological staining of reactive mesothelium, mesothelioma, and lung carcinoma with a panel of monoclonal antibodies.

Ghosh¹,

Gatter²,

Dunnill³

et al. 1987

J Clin Pathol

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Section: Discussionmentioning

confidence: 80%

Immunohistological staining of reactive mesothelium, mesothelioma, and lung carcinoma with a panel of monoclonal antibodies.

Ghosh¹,

Gatter²,

Dunnill³

et al. 1987

J Clin Pathol

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“…Unfortunately NCRC-I I stains mesothelial cells, particularly in a reactive state, and would be of no value in this area. Indeed doubt has been cast on one of these antibodies, Ca I, which has not proved to be reliable in this area in other laboratories, staining reactive mesothelium sometimes more frequently than malignant cells (Burnet et al 1983).…”

Section: Controlsmentioning

confidence: 99%

A monoclonal antibody, NCRC‐11, raised to human breast carcinoma. 1. Production and immunohistological characterization

et al. 1984

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The production of a mouse monoclonal IgM antibody, NCRC-11, raised against human breast carcinoma is described. It has been characterized immunohistologically. The antigen recognised has a wide but highly specific distribution in normal tissues, being virtually confined to the surface of certain epithelial cell types. It is found in some forms of epithelial metaplasia and most epithelial malignancies, particularly adenocarcinomas. The heterogeneity of staining in mammary carcinomas is outlined and is of particular interest. The immunohistological staining distribution of NCRC-11 is similar to other antibodies, including anti-epithelial membrane antigen, which were raised against human milk fat globule membrane. A competition experiment with some of these antibodies, using a flow cytofluorimeter, showed competition with one antibody, LICR LON/M8.

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Ca2 and Ca3 new monoclonal antibodies evaluated as tumor markers in serous effusions

Bramwell

Ghosh

Smith

et al. 1985

Cancer

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Ca2 and Ca3 are new monoclonal antibodies of IgG1 class, directed against the Ca antigen, a mucustype glycoprotein expressed on the surface of a wide range of malignant human cells and certain specialized normal epithelia. These antibodies were produced by immunization with purified preparations of the Ca antigen. They were tested to assess their value in the diagnosis of malignant effusions. Immuno‐alkaline‐phosphatase staining was used. Smears of pleural and peritoneal effusions were chosen to show: (1) undoubted malignant cells of various types; and (2) mesothelial cells in effusions from cases in which cancer was not in question. The Ca2 antibody, at 1 in 20 dilution of the culture supernatant, was the most specific, giving no reactions with benign mesothelial cells from any of the 35 cases tested. Malignant cells were clearly stained in 35 of 40 cases of carcinoma or mesothelioma. The staining was negative in two cases of oat cell bronchial carcinoma, and in three of four cases of carcinoma of the colon. Ca3 gave similar, but somewhat stronger, reactions with carcinoma cells, but was less specific, reacting weakly with mesothelial cells in 8 of 35 benign effusions. Because the falsenegative reactions given by the Ca series of antibodies are to some extent complementary to those given by monoclonal antibodies directed against the carcinoembryonic antigen (CEA), a combination of Ca2 and anti‐CEA is recommended as a most useful addition to the normal cytologic examination of effusions.

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EVALUATION OF Ca1 ANTIBODY IN PLEURAL BIOPSY MATERIAL

Cited by 9 publications

References 2 publications

Immunohistological staining of reactive mesothelium, mesothelioma, and lung carcinoma with a panel of monoclonal antibodies.

Immunohistological staining of reactive mesothelium, mesothelioma, and lung carcinoma with a panel of monoclonal antibodies.

A monoclonal antibody, NCRC‐11, raised to human breast carcinoma. 1. Production and immunohistological characterization

Ca2 and Ca3 new monoclonal antibodies evaluated as tumor markers in serous effusions

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