Immunohistological staining of reactive mesothelium, mesothelioma, and lung carcinoma with a panel of monoclonal antibodies.

Ghosh, Anna K; Gatter, K C; Dunnill, M. S.; Mason, David Y.

doi:10.1136/jcp.40.1.19

Cited by 54 publications

(26 citation statements)

References 20 publications

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“…CEA was often used in immunohistochemical studies to distinguish carcinoma cells from reactive mesothelial or mesothelioma cells (14,28). However, it was never used as a RT-PCR marker in serous effusions.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of a Panel of Molecular Markers for the Diagnosis of Malignant Serous Effusions

Passebosc-Faure

Lambert

et al. 2005

Clinical Cancer Research

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Purpose: Our main goal was to evaluate a panel of molecular markers for the detection of cancer cells in serous effusions and to determine their value as an adjunctive reverse transcription-PCR (RT-PCR) test to cytologic examination. Experimental Design: One hundred fourteen serous effusions from 71patients with tumors and 43 patients with benign diseases were subjected to RT-PCR for expression of carcinoembryonic antigen (CEA), epithelial cell adhesion molecule (Ep-CAM), E-cadherin, mammaglobin, mucin 1 (MUC1) isoforms MUC1/REP, MUC1/Y, and MUC1/Z, calretinin, and Wilms' tumor 1susceptibility gene. Results: CEA, Ep-CAM, E-cadherin, and mammaglobin were specifically expressed in malignant effusions. The sensitivity of RT-PCR in cytologically negative malignant effusions was 63.1% combining CEA and Ep-CAM (with 100% specificity) and reached 78.9% adding MUC1/Y or MUC1/Z (with 93% specificity). In the whole population of effusions, the combination of cytology with RT-PCR of CEA and Ep-CAM yielded a 90.1% sensitivity, a specificity and a positive predictive value of 100%, and a 86% negative predictive value for malignancy. Adding MUC1/Y or MUC1/Z to the panel, the sensitivity was 94.5% with 93% specificity, 95.7% PPV, and 90.9% negative predictive value. Moreover, CEA and mammaglobin were specifically expressed in epithelial malignancies, and mammaglobin was mainly expressed in effusions from breast carcinoma (97.3% of specificity). Conclusions: A combination of cytology and RT-PCR analysis of CEA and Ep-CAM significantly improved the detection sensitivity of tumor cells in serous effusions. RT-PCR analysis of CEA, Ep-CAM, and mammaglobin in serous effusions could be a beneficial adjunct to cytology for the diagnosis of malignancy.

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Section: Discussionmentioning

confidence: 99%

Evaluation of a Panel of Molecular Markers for the Diagnosis of Malignant Serous Effusions

Passebosc-Faure

Lambert

et al. 2005

Clinical Cancer Research

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“…Cultures in which TC either failed to grow or were initially absent were used as a source of MC. Characterisation involved recognition of the following criteria, some of which have been described in the literature (Mouriquand et al, 1978;Whitehead & Hughes, 1975) (Wilson et al, 1987), although there are varied reports in the literature which describe positive staining of some benign MC in wax-embedded tissue (Ghosh et al, 1987) and negative staining in wet-fixed smears (Epenetos et al, 1982). MC used in this study stained positively for both vimentin and keratin, as has been previously reported (Connell & Rheinwald, 1983) whereas epithelial cells were, until recently, believed to express only keratin.…”

Section: Tumour Cellsmentioning

confidence: 99%

Mesothelial cells stimulate the anchorage-independent growth of human ovarian tumour cells

Wilson

1989

Br J Cancer

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“…Carcinoembryonic antigen has been consistently reported to have high frequency of positivity in lung carcinomas, particularly adenocarcinomas (26,27). Reports of CEA immunoreactivity for epithelial ovarian carcinomas, however, are conflicting, ranging from 0% to as high as 69% in serous carcinoma in different series (28).…”

Section: N R Howell Et Al 138mentioning

confidence: 97%

Carcinomas of Ovary and Lung With Clear Cell Features

Howell

Zheng

Liu

et al. 2007

International Journal of Gynecological Pathology

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Metastatic lung carcinomas with clear cell morphology can be confused with primary ovarian clear cell carcinomas. We performed immunohistochemical stains in 14 cases of non-small cell lung carcinomas with clear cell features and 14 cases of ovarian clear cell carcinomas using a panel of markers, including thyroid transcription factor 1 (TTF-1), carcinoembryonic antigen (CEA), Wilms tumor gene 1, octamer-binding transcription factor 4 (OCT-4), cancer antigen 125 (CA-125), estrogen receptor, and progesterone receptor. Among non-small cell lung carcinomas with clear cell features, 87.5% of adenocarcinomas (or 50% overall frequency in lung carcinomas) were positive for TTF-1, whereas none of the ovarian clear cell carcinomas were positive (P = 0.002). All 14 ovarian clear cell carcinomas stained for CA-125 as compared with 1 non-small cell lung carcinoma (P < 0.001). On the other hand, 85% of non-small cell lung carcinomas stained for CEA, whereas none of the ovarian clear cell carcinomas did (P < 0.001). Interestingly, 4 ovarian clear cell carcinomas (28%) showed positive staining for the germ cell marker OCT-4. Either lung or ovarian carcinomas stained for Wilms tumor gene 1, estrogen receptor, or progesterone receptor very infrequently; and the difference between the 2 groups was not statistically significant. Our results suggest that an immunohistochemical panel consisting of TTF-1, CEA, CA-125, and OCT-4 is helpful in distinguishing most pulmonary and ovarian carcinomas with clear cell features.

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Immunohistological staining of reactive mesothelium, mesothelioma, and lung carcinoma with a panel of monoclonal antibodies.

Cited by 54 publications

References 20 publications

Evaluation of a Panel of Molecular Markers for the Diagnosis of Malignant Serous Effusions

Evaluation of a Panel of Molecular Markers for the Diagnosis of Malignant Serous Effusions

Mesothelial cells stimulate the anchorage-independent growth of human ovarian tumour cells

Carcinomas of Ovary and Lung With Clear Cell Features

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