Evaluation of Bleach Sedimentation Technique in Detection of Mycobacterium tuberculosis by LED Microscopy

Yadav, Suneet; Sujatha, R.; Singh, Deepshikha; Sameer, Deepak

doi:10.20546/ijcmas.2018.707.401

Cited by 1 publication

(1 citation statement)

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“…The process of denaturation of sputum constituents also leads to flocculation with subsequent increase in the sedimentation rate of mycobacteria. 11 The standard method for concentration is the NALC-NaOH (N-acetyl-L-cysteine-sodium hydroxide) method which is technically demanding for a remote laboratory, and costs about 1 USD (INR 80 approx) per clinical specimen. 12 NaOCl is however available at prices almost negligible (INR 1 / sample) as compared to NALC-NaOH method.…”

Section: Discussionmentioning

confidence: 99%

Role of Bleach Concentration Method for Detection of Acid-fast Bacilli (AFB) in Sputum using Conventional Ziehl-Neelsen (ZN) Staining Technique

Pal¹,

Shenoy²,

Bhattacharya³

2023

J. Pure Appl. Microbiol.

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Sputum microscopy is the most important tool for the detection of Mycobacterium tuberculosis in peripheral laboratories. Sodium hypochlorite concentration technique prior to sputum microscopy may improve the detection of AFB bacilli over direct Ziehl Neelsen’s staining method. A prospective study was conducted at Kasturba Medical College, Manipal. Consecutive sputum samples were collected for 3 months. ZN stained smears were made directly of fresh specimens and of specimens that were processed with 3.5% sodium hypochlorite (NaOCl), from each patient. Then, ZN stained smears were observed under light microscopy. A total of 239 samples were collected. The yield of positive smears with ZN staining after NaOCl centrifugation was higher than that with microscopy after direct ZN stain. The percentage increase yield of ZN staining after NaOCl centrifugation over direct ZN staining was 3.3% (8/239). Bleach centrifugation can help in increasing the yield of positivity in sputum smear.

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Section: Discussionmentioning

confidence: 99%